A5103660772- CRISPR and Genetic Engineering
- Zebrafish Biomedical Research Applications
- Gut microbiota and health
- Aquaculture disease management and microbiota
- Cardiomyopathy and Myosin Studies
- Neuroendocrine regulation and behavior
- Pluripotent Stem Cells Research
- Insect behavior and control techniques
- Veterinary Pharmacology and Anesthesia
- Diptera species taxonomy and behavior
- Clostridium difficile and Clostridium perfringens research
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Dietary Effects on Health
- Birth, Development, and Health
- RNA modifications and cancer
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Probiotics and Fermented Foods
- Tryptophan and brain disorders
- Respiratory Support and Mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Adipose Tissue and Metabolism
- Pain Mechanisms and Treatments
- Neurogenetic and Muscular Disorders Research
University of Missouri
2015-2025
Missouri College
2021-2025
University of Wyoming
2020
Delft University of Technology
2017
Michigan State University
2017
Wright State University
2012-2015
University of Cambridge
2014
Iowa Methodist Medical Center
2008
The FDA approved drug rapamycin increases lifespan in rodents and delays age-related dysfunction humans. Nevertheless, important questions remain regarding the optimal dose, duration, mechanisms of action context healthy aging. Here we show that 3 months treatment is sufficient to increase life expectancy by up 60% improve measures healthspan middle-aged mice. This transient also associated with a remodeling microbiome, including dramatically increased prevalence segmented filamentous...
Abstract The consumption of probiotics has become increasingly popular as a means to try improve health and well-being. Not only are considered beneficial digestive health, but increasing evidence suggests direct indirect interactions between gut microbiota (GM) the central nervous system (CNS). Here, adult zebrafish were supplemented with Lactobacillus plantarum determine effects probiotic treatment on structural functional changes GM, well host neurological behavioral changes. L....
The influence of the microbiota on behavior and stress responses is poorly understood. Zebrafish larvae have unique characteristics that are advantageous for neuroimmune research, however, they currently underutilized such studies. Here, we used germ-free zebrafish to determine effects testing. absence a dramatically altered locomotor anxiety-related behavior. Additionally, characteristic an acute stressor were also obliterated in lacking exposure microbes. Lastly, treatment with probiotic...
In this paper, we demonstrate a first-of-its-kind adaptive intervention in MOOC utilizing real-time clickstream data and novel machine learned model of behavior. We detail how augmented the edX platform with capabilities necessary to support type which required both tracking learners' behaviors dynamically adapting content based on each learner's individual history. Our chosen pilot was category pathways courseware took form navigational suggestion appearing at bottom every non-forum page...
During the ejection phase of cardiac cycle, left ventricular (LV) myocytes undergo loaded shortening and generate power. However, few studies have measured sarcomere during contractions. Here, we simultaneously monitored muscle length (ML) (SL) isotonic contractions in rodent permeabilized LV myocyte preparations. In preparations from rats, found that ML SL traces were closely matched, as velocities within ∼77% half-maximal Ca2+ activations. We next tested whether myosin binding protein-C...
Electrotaxis, directional cell movement in response to an electric potential, has been demonstrated a wide range of types including lymphocytes. Exoelectrogens, microorganisms capable generating electrical currents, have identified microbial fuel cells. However, no studies investigated exoelectrogenic microbes fresh feces or the effects microbiota on host organism. Here we show that commensal gut populations differ their capacity for current production by exoelectrogens and those differences...
Neuropathic pain is a devastating condition where current therapeutics offer little to no relief. Novel nonnarcotic therapeutic targets are needed address this growing medical problem. Our work identified the G-protein-coupled receptor 160 (GPR160) as potential target for intervention. However, lack of small-molecule ligands GPR160 hampers our understanding its role in health and disease. To void, we generated global Gpr160 knockout (KO) mouse using CRISPR-Cas9 genome editing technology...
Abstract Spinal muscular atrophy with respiratory distress type I (SMARD1) is a neurodegenerative disease defined by distress, muscle and sensory autonomic nervous system defects. SMARD1 result of mutations within the IGHMBP2 gene. We have generated six Ighmbp2 mouse models based on patient-derived that in and/or Charcot-Marie Tooth Type 2 (CMT2S). Here we describe characterization one these models, Ighmbp2D564N (human D565N). The Ighmbp2D564N/D564N model mimics important aspects phenotype,...
Abstract The Cre/ lox P recombination system has revolutionized the ability to genetically manipulate animal genomes in order conditionally control gene expression. With recent advances genome editing, barriers manipulating rat have been overcome and it is now possible generate new strains (Cre drivers) which Cre recombinase expression carefully controlled temporally and/or spatially. However, evaluate characterize these driver limited by availability of reliable reporter strains. Here, we...
Genome editing technology is widely used to produce genetically modified animals, including rats. Cytoplasmic or pronuclear injection of DNA repair templates and CRISPR-Cas reagents the most common delivery method into embryos. However, this type micromanipulation necessitates access specialized equipment, laborious, requires a certain level technical skill. Moreover, microinjection techniques often result in lower embryo survival due mechanical stress on embryo. In protocol, we developed an...
The microorganisms colonizing the gastrointestinal tract of animals, collectively referred to as gut microbiome, affect numerous host behaviors dependent on central nervous system (CNS). Studies comparing germ-free mice normally colonized have demonstrated influences microbiome anxiety-related behaviors, voluntary activity, and gene expression in CNS. Additionally, there is epidemiologic evidence supporting an intergenerational influence maternal neurodevelopment offspring behavior later...
Data presented here contains metagenomic analysis regarding the sequential conventionalization of germ-free zebrafish embryos. Zebrafish embryos that underwent a sterilization process immediately after fertilization were promptly exposed to and raised larval stage in conventional fish water. At 6 days postfertilization (dpf), these "conventionalized" larvae compared water never undergoing initial process. Bacterial 16S rRNA amplicon sequencing was performed on DNA isolated from homogenates...
Abstract In this study, the transcriptional repressor REST (Repressor Element 1 Silencing Transcription factor) was ablated in mouse placenta to investigate molecular and cellular impacts on offspring brain at different life stages. Ablation of placental deregulated several metabolites, including glucose lactate that fuel energy, vitamin C (ascorbic acid) functions epigenetic programming during postnatal development, glutamate creatine help respond stress conditions adult life. Bulk RNA‐seq...
Abstract Background Zebrafish used in research settings are often housed recirculating aquaculture systems (RAS) which rely on the system microbiome, typically enriched a biofiltration substrate, to remove harmful ammonia generated by fish via oxidation. Commercial RAS must be allowed equilibrate following installation, before can introduced. There is little information available regarding bacterial community structure commercial zebrafish housing systems, or time-point at biofilter reaches...