A5070204346- Cholangiocarcinoma and Gallbladder Cancer Studies
- bioluminescence and chemiluminescence research
- Cancer, Lipids, and Metabolism
- Fluorine in Organic Chemistry
- Advanced biosensing and bioanalysis techniques
- Cancer, Hypoxia, and Metabolism
- Nanoplatforms for cancer theranostics
- Hepatocellular Carcinoma Treatment and Prognosis
- Synthesis and Reactions of Organic Compounds
- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Peptidase Inhibition and Analysis
- Orthopedic Infections and Treatments
- Calcium signaling and nucleotide metabolism
- Protease and Inhibitor Mechanisms
- Photoacoustic and Ultrasonic Imaging
- Cyclopropane Reaction Mechanisms
- Gallbladder and Bile Duct Disorders
- Cancer Cells and Metastasis
- 3D Printing in Biomedical Research
- CRISPR and Genetic Engineering
- Biotin and Related Studies
- Synthesis and Biological Evaluation
- Neuroendocrine regulation and behavior
- Pancreatic function and diabetes
University of Missouri
2021-2025
École Polytechnique Fédérale de Lausanne
2016-2022
Taras Shevchenko National University of Kyiv
2019
FujiFilm VisualSonics (Canada)
2016
Johns Hopkins University
2016
Regeneron (United States)
2016
Community Options (United States)
2016
Pfizer (United States)
2016
Stemnion (United States)
2016
The microbiome-produced enzyme bile salt hydrolase (BSH) plays a central role in human health, but its function remains unclear due to the lack of suitable methods for measuring activity. Here, we have developed novel optical tool based on ultrasensitive bioluminescent imaging and demonstrated that this assay can be used quick cost-effective quantification BSH activity across broad range biological settings including pure enzymes bacteria, intact fecal slurries, noninvasive live animals, as...
Despite advances in breast cancer therapy, effective targeting of stem cells (CSCs) remains a challenge. CSCs, which have self-renewal, tumorigenic and metastatic properties, are often quiescent located hypoxic regions tumors [1], making them resistant to conventional chemo- radiotherapies [2]. These characteristics allow CSCs survive, leading relapse metastasis. Studying under conditions similar their niche is essential for evaluating therapies that target these cells. We show can be...
Bioluminescent imaging (BLI) is one of the most powerful and widely used preclinical modalities. However, current technology relies on use transgenic luciferase-expressing cells animals therefore can only be applied to a limited number existing animal models human disease. Here, we report development "portable bioluminescent" (PBL) that overcomes major limitations traditional BLI. We demonstrate PBL method capable noninvasive measuring activity both extracellular (e.g., dipeptidyl peptidase...
Diagnosing prosthetic joint infection (PJI) poses significant challenges, and current modalities are fraught with low sensitivity and/or potential morbidity. Photoacoustic imaging (PAI) is a novel ultrasound-based modality for diagnosing PJI safely noninvasively.In an established preclinical mouse model of bioluminescent Staphylococcus aureus PJI, fluorescent indocyanine green (ICG) was conjugated to β-cyclodextrin (CDX-ICG) or teicoplanin (Teic-ICG) injected intravenously 1 week...
Cancer stem cells (CSCs) drive tumorigenesis, are responsible for metastasis, and resist conventional therapies thus posing significant treatment challenges. CSCs reside in hypoxic tumor regions therefore, effective must target within this specific microenvironment. characterized by limited distinguishable features, however, surface displayed phosphatidylserine (PS) appears to be characteristic of offers a potential target. GlaS, truncated coagulation protein that is internalized after...
Abstract Altered lipid metabolism is a common hallmark of various cancers, including intrahepatic cholangiocarcinoma (ICC), highly lethal carcinoma that lacks effective treatment options. To elucidate the changes in ICC, we coupled expression firefly luciferase gene (FFL) to AKT1 (AKT-FFL) via an IRES linker, and then hydrodynamically injected mice with AKT-FFL Notch1 intracellular cytoplasmic domain (NICD) establish luciferase-positive ICC model. This model not only enabled us monitor...
The microorganisms colonizing the gastrointestinal tract of animals, collectively referred to as gut microbiome, affect numerous host behaviors dependent on central nervous system (CNS). Studies comparing germ-free mice normally colonized have demonstrated influences microbiome anxiety-related behaviors, voluntary activity, and gene expression in CNS. Additionally, there is epidemiologic evidence supporting an intergenerational influence maternal neurodevelopment offspring behavior later...
Abstract The kidney’s inherent complexity has made identifying cell-specific pathways challenging, particularly when temporally associating them with the dynamic pathophysiology of acute kidney injury (AKI). Here, we combine renal luciferase reporter mice using a chemoselective luciferin to guide acquisition transcriptional changes in C57BL/6 background mice. Hydrogen peroxide generation, common mechanism tissue damage, was tracked peroxy-caged-luciferin identify optimum time points for...
<p>Gene expression levels of FATP5 were measured using qPCR in 5 normal livers (NL), 33 ICC, and related surrounding (SL).</p>
<p>Gene expression levels of Fatp5, and Fasn were measured using qPCR in AAV-shSCR AAV-shFatp5 ICC tumors.</p>
<div>Abstract<p>Altered lipid metabolism is a common hallmark of various cancers, including intrahepatic cholangiocarcinoma (ICC), highly lethal carcinoma that lacks effective treatment options. To elucidate the changes in ICC, we coupled expression firefly luciferase gene (FFL) to <i>AKT1</i> (<i>AKT-FFL</i>) via an IRES linker, and then hydrodynamically injected mice with AKT-FFL <i>Notch1</i> intracellular cytoplasmic domain...
<div>Abstract<p>Altered lipid metabolism is a common hallmark of various cancers, including intrahepatic cholangiocarcinoma (ICC), highly lethal carcinoma that lacks effective treatment options. To elucidate the changes in ICC, we coupled expression firefly luciferase gene (FFL) to <i>AKT1</i> (<i>AKT-FFL</i>) via an IRES linker, and then hydrodynamically injected mice with AKT-FFL <i>Notch1</i> intracellular cytoplasmic domain...
<p>Gene expression levels of FATP5 were measured using qPCR in 5 normal livers (NL), 33 ICC, and related surrounding (SL).</p>
<p>Gene expression levels of Fatp5, and Fasn were measured using qPCR in AAV-shSCR AAV-shFatp5 ICC tumors.</p>
Cathepsin D is an aspartic protease and one of the most abundant proteases. It overexpressed in many cancers plays important role tumor development, progression, metastasis. While it a physiologically intracellular protein, cathepsin secreted into extracellular matrix under pathological conditions, making appealing target for drug delivery systems. Here, we present development evaluation new system targeting based on immunoliposomes functionalized with pepstatin A—a natural peptide inhibitor...
Abstract BACKGROUND Glioblastoma (GBM) is the most aggressive primary tumor of brain with a median survival 15 months. Maximal safe resection initial standard-of-care, but limited by diffuse invasiveness tumor. Fluorescence imaging has been shown to aid intra-operative identification, current options fail do so consistently. Recent literature fatty acid (FA) metabolism and uptake be altered in glioblastoma. For this reason, we introduce novel, near-infrared fluorescent dye, fatty-acid...