A5081223834- Neurosurgical Procedures and Complications
- Spine and Intervertebral Disc Pathology
- Intracranial Aneurysms: Treatment and Complications
- Intracerebral and Subarachnoid Hemorrhage Research
- Spinal Fractures and Fixation Techniques
- Head and Neck Surgical Oncology
- Cervical and Thoracic Myelopathy
- Neuroinflammation and Neurodegeneration Mechanisms
- Cytokine Signaling Pathways and Interactions
- Meningioma and schwannoma management
- Nitric Oxide and Endothelin Effects
- Traumatic Brain Injury and Neurovascular Disturbances
- Cerebrovascular and genetic disorders
- S100 Proteins and Annexins
- Macrophage Migration Inhibitory Factor
- Spinal Hematomas and Complications
- Connective tissue disorders research
- Neuroscience and Neuropharmacology Research
- Spinal Cord Injury Research
- Axon Guidance and Neuronal Signaling
- Immune cells in cancer
- Ear and Head Tumors
- Angiogenesis and VEGF in Cancer
- Cancer, Hypoxia, and Metabolism
- Nerve Injury and Rehabilitation
Aichi Medical University
2015-2024
Neurological Surgery
2013-2023
Kanazawa Medical University
2021-2022
Oxford University Press (United Kingdom)
2020
Aichi Medical University Hospital
2012-2015
Asahi General Hospital
2015
The University of Tokyo
2008-2011
Nagoya University
1996-2007
Fujita Health University
2004
Kagawa University
2004
The Janus kinase (JAK)/signal transducer and activator of transcription (STAT) signalling pathway is one the most important in transducing signals from cell surface to nucleus response cytokines. In present study, we investigated chronological alteration cellular location JAK1, STAT3, phosphorylated (p)-Tyr1022/1023-JAK1, p-Tyr705-STAT3, interleukin-6 (IL-6) following spinal cord injury (SCI) mice. Western blot analysis showed JAK1 be significantly at Tyr1022/1023 6 h after SCI, peaking 12...
Interleukin-6 (IL-6), an inflammatory cytokine, plays important roles in cerebrospinal fluid (CSF) after subarachnoid hemorrhage (SAH). Chemokines are chemoattractant cytokines that regulate trafficking of monocytes/macrophages and lymphocytes to sites inflammation. However, no studies have been reported regarding the temporal expression these CSF SAH. The concentrations IL-6, monocyte protein-1 (MCP-1), interferon-γ-inducible protein-10 (IP-10), monokine induced by interferon-γ (MIG) ten...
The locomotive syndrome risk test was developed to quantify the decrease in mobility among adults, which could eventually lead disability. purpose of this study establish reference values for adults and investigate influence age sex.We analyzed 8681 independent community dwellers (3607 men, 5074 women). Data pertaining (the two-step test, stand-up 25-question geriatric function scale [GLFS-25]) scores were collected from seven administrative areas Japan.The three generated all gradually...
The intravenous infusion of rat adrenomedullin, at concentrations ranging from 0.1 to 1.0 microgram/kg/min, for 60 min increased the regional cerebral blood flow (rCBF) in a dose-dependent manner rats. rCBF was measured using laser Doppler flowmetry device placed on surface parietal cortex. increase induced by microgram/kg/min adrenomedullin up 145 +/- 10.8% controls (n = 5, p < 0.001). These did not affect systemic pressure or other physiologic parameters, including pH, PaCO2, PaO2,...
INTRODUCTION: Spinal cord injury (SCI) precipitates profound enduring deficits. Recently, cell transplantation therapy has emerged as a noteworthy intervention for SCI. Although stem cells derived from human exfoliated deciduous teeth (SHED) present an enticing therapeutic prospect, their precise mode of action remains elusive. METHODS: Employing rat clip compression SCI model, we partitioned the animals randomly into three cohorts: SCI, + PBS, and SHED. Subsequent to induction, assessed...
Taurine has multiple functions in the central nervous system (CNS), serving as an osmoregulator, antioxidant, inhibitory neuromodulator, and regulator of intracellular Ca2+ flux. Since role taurine traumatic spinal cord injury (SCI) is not fully understood, present study was conducted with C57 black/6 mice (18–20 g) who underwent severe SCI at Th-8 level using a weight compression device. injected intraperitoneally doses 25, 80, 250, 800 mg/kg within 30 min after SCI. Controls were saline....
Tamoxifen (TAM), a widely used non‐steroidal anti‐estrogen, has recently been shown to be neuroprotective in rat model of reversible middle cerebral artery occlusion (rMCAo). several potential mechanisms action including inhibition the release excitatory amino acids (EAA) and nitric oxide synthase (NOS) activity. The question addressed this study was whether TAM reduces ischemia‐induced production nitrotyrosine, considered as footprint product superoxide, peroxynitrite . In brain, 2 h rMCAo...
Previous studies showed that complement activation is associated with poor functional outcome after aneurysmal subarachnoid hemorrhage (SAH). We investigated whether underlying brain injury SAH (n = 7) and if it an appropriate treatment target. expression in tissue of patients 930) studied the role common genetic variants C3 C5 genes outcome. analyzed plasma levels 229) to identify functionality a single nucleotide polymorphism (SNP) The time course C5a was measured 31) CSF 10). In mouse...
Spinal cord injury (SCI) induces devastating permanent deficits. Recently, cell transplantation therapy has become a notable treatment for SCI. Although stem cells from human exfoliated deciduous teeth (SHED) are an attractive therapy, their precise mechanism of action remains to be elucidated. In this study, we explored one the neuroprotective mechanisms SHED at subacute stage after We used rat clip compression SCI model. The animals were randomly divided into three groups: SCI, +...
The authors report a simple method for bilateral open-door cervical expansive laminoplasty in which hydroxyapatite (HA) spacers are secured by titanium screws. A biomechanical study was also conducted to confirm the strength of screw fixation.A unilateral posterior approach used allow preservation supporting elements (the tension band) until laminae were cut at base. laminotomy then performed standard fashion. Appropriate-sized HA selected, held with specially designed holder, and placed...
Chronic subdural hematoma (CSDH) is an angiogenic disease that recognized as a cause of treatable dementia with unknown pathogenesis. Vascular endothelial growth factor (VEGF), potent regulating angiogenesis through the phosphatidylinositol 3-kinase (PI3-kinase)/Akt pathway, has been implicated in its etiology. The status this signaling pathway CSDH outer membranes was examined present study, using obtained during trepanation surgery. Expressions PI3-kinase, PKB-kinase, Akt, phosphorylated...
Severe subarachnoid hemorrhage (SAH) induces dysfunction of endothelial nitric oxide synthase (eNOS), resulting in severe vasospasm. Clinically, however, some portions cerebral arteries may show only mild Although vasospastic after SAH have been intensively studied, activity eNOS associated with the form disease has received less attention. The purpose present study was to clarify molecular mechanisms underlying regulation SAH. In a rat single-hemorrhage model, basilar were obtained up 7...
To investigate nitric oxide (NO) metabolism after subarachnoid hemorrhage (SAH).We measured the concentrations of NO metabolites, nitrite and nitrate, in cerebrospinal fluid (CSF) obtained from cisternal drainage patients with SAH. Studies were performed for 31 who had undergone surgical obliteration bleeding aneurysms within 3 days their hemorrhage. The nitrate CSF 14 using a nitrate/nitrite kit samples that on daily basis drainage.Compared control values (2.6 +/- 0.4 mumol/L, n = 14)...
The authors previously demonstrated that Ca 2+ /calmodulin (CaM)-dependent protein kinase IIα (CaM-KIIα) can phosphorylate neuronal nitric oxide synthase (nNOS) at Ser 847 and attenuate NOS activity in cells. In the present study, they established forebrain ischemia causes an increase phosphorylation of nNOS hippocampus. This appeared to be catalyzed by CaM-KII: (1) it correlated with autophosphorylation CaM-KIIα; (2) was blocked CaM-KII inhibitor, KN-93; (3) CaM-KIIα were found coexist...
Background and Purpose —Inducible cyclooxygenase (COX-2) has been found to play a pathological role in cerebral insult . We investigated the expression of COX-2 basilar artery after experimental subarachnoid hemorrhage (SAH). Methods —In canine “two-hemorrhage” model SAH, arteries were obtained on day 2 cisternal injection autologous blood or days 4, 6, 7, 9 second injection. Basilar also 12 hours intracisternal cytokine: interleukin (IL)-1β (0.03 μg), IL-6 (3 IL-8 (10 μg). Western blotting...
Chronic subdural hematoma (CSDH) is considered to be an inflammatory disease. Eosinophils are frequently expressed in the outer membrane of CSDH and major sources transforming growth factor beta (TGF-β). The mothers against decapentaplegic (Smad)-signaling pathway, which activated by TGF-β, has been shown involved with fibrosis. In present study, we compared concentrations eotaxin-3, eosinophil-specific chemoattractant, TGF-β between fluid cerebrospinal (CSF) from control patients. We also...
Production of NO by endothelial synthase (eNOS) is thought to play a neuroprotective role after cerebral ischemia. The vascular growth factor (VEGF) contributes activation eNOS Ca2+/calmodulin and also stimulates the protein kinase Akt, which directly phosphorylates on Ser1177 increases enzyme activity. Although expression VEGF has been studied in ischemic stroke models, ischemia not investigated. purpose present study was clarify molecular mechanisms underlying regulation activity through...
Growth factors and inflammatory cytokines activate the mitogen-activated protein kinase (MAPK) cascade. Previous studies have shown that chronic subdural hematoma (CSDH) fluid contains these cytokines. In this study, expression of three major MAPK cascade transmitters in outer membrane CSDH was assessed. Eleven patients whose were successfully obtained during trepanation surgery included study. Expression extracellular signal-regulated (ERK), phosphorylated (p)-ERK, p38, p-p38, c-Jun...