A5100389279- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Bacteriophages and microbial interactions
- HIV Research and Treatment
- HER2/EGFR in Cancer Research
- Immune Cell Function and Interaction
- Escherichia coli research studies
- Virus-based gene therapy research
- Protein purification and stability
- Viral gastroenteritis research and epidemiology
- Cancer Research and Treatments
- Toxin Mechanisms and Immunotoxins
- Polyomavirus and related diseases
- RNA and protein synthesis mechanisms
- Radiopharmaceutical Chemistry and Applications
- CRISPR and Genetic Engineering
- Fungal and yeast genetics research
- Cell Adhesion Molecules Research
- Genomics and Chromatin Dynamics
- Cytomegalovirus and herpesvirus research
- Allergic Rhinitis and Sensitization
- Viral Infections and Outbreaks Research
- Viral Infections and Vectors
- HIV/AIDS drug development and treatment
- Hepatitis B Virus Studies
Hua Yuan Group (China)
2024
Wen's Food Group (China)
2023
Joyoung (China)
2023
Ningbo University
2023
University of Chinese Academy of Sciences
2023
Blood Center of Zhejiang Province
2023
University of Toronto
2010-2022
First Affiliated Hospital of Xinjiang Medical University
2021-2022
Xinjiang Medical University
2021-2022
Donnelly College
2021
Random mutagenesis and selection using phage or cell surface display provides an efficient method for affinity maturation of single chain Fv (scFv) antibodies, thereby improving function in various applications. To investigate the effects mutation frequency on maturation, error-prone PCR was used to generate libraries containing average ( m ) between 1.7 22.5 base substitutions per gene a high scFv antibody that binds cardiac glycoside digoxigenin. The were displayed Escherichia coli ,...
Abstract EBV, a ubiquitous human herpesvirus, is the causative agent of infectious mononucleosis and associated with many carcinomas. We have previously shown that EBV latent genes LMP-1 LMP-2A (for membrane proteins 1 2A), transactivate endogenous retrovirus (HERV), HERV-K18, in infected B lymphocytes. The envelope (Env) protein HERV-K18 encodes superantigen strongly stimulates large number T cells. In this study we report env transactivated even earlier infection process, before...
Background: SEB is a potent superantigen that can cause toxic shock in humans and be incapacitating lethal when used as bioweapon.Results: Therapeutic administration of highly affinity-matured synthetic anti-SEB human monoclonal IgGs protects mice from challenge.Conclusion: Affinity maturation enhance neutralizing efficacy lead to successful postexposure therapeutics.Significance: No vaccines or therapeutics are currently available against SEB. bioweapon. Results: challenge. Conclusion:...
Abstract Cancer therapy faces numerous challenges that can limit the effectiveness of treatment. In order to address these key hurdles, such as immunosuppression, tumor heterogeneity, and likelihood resistance, offer longer-lasting control tumor, we’ve developed a novel dual drug ADC (BiADC), BR113, by conjugating an anti-hTrop2 antibody with linker Topoisomerase I inhibitor payload, another immunostimulator payload. The approach combining direct cytotoxic effects ADCs immune-enhancing...
RNA plays a pivotal role in diverse cellular functions across organisms. Developing computational algorithms for sequence related questions is highly valuable. Recently, genomic language models (gLMs) with pre-training have emerged, offering flexibility various downstream prediction tasks. However, comprehensive and fair evaluations of gLMs are lacking. In this study, we benchmark eight on tasks covering four processes, highlighting their strengths limitations. While excel performance...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTGeneration of Highly Enantioselective Catalysts from the Pseudoenatiomeric Assembly BINOL, F8BINOL, and Ti(OiPr)4Subramanian Pandiaraju, Gang Chen, Alan Lough, Andrei K. YudinView Author Information Department Chemistry, University Toronto 80 St. George St., Toronto, Ontario M5S 3H6 Cite this: J. Am. Chem. Soc. 2001, 123, 16, 3850–3851Publication Date (Web):March 28, 2001Publication History Received17 November 2000Published online28 March...
The recombinant expression of immunoglobulin domains, Fabs and scFvs in particular, Escherichia coli can vary significantly from antibody to antibody. We hypothesized that poor Fab is often linked intrinsic stability. To investigate this further, we applied a novel approach for stabilizing poorly expressing anti-tetanus toxoid human with predisposition being misfolded non-functional. Forty-five residues within the were chosen saturation mutagenesis based on residue frequency analysis...
Shiga-like toxins are ribosome-inactivating proteins (RIP) produced by pathogenic E. coli strains that responsible for hemorrhagic colitis and hemolytic uremic syndrome. The catalytic A1 chain of toxin 1 (SLT-1), a representative RIP, first docks onto conserved peptide SD[D/E]DMGFGLFD located at the C-terminus all three eukaryotic ribosomal stalk halts protein synthesis through depurination an adenine base in sarcin-ricin loop 28S rRNA. Here, we report SLT-1 rapidly binds to dissociates from...
Background Stx toxin is a member of the AB5 family bacterial toxins: active A subunit has N-glycosidase activity against 28S rRNA, resulting in inhibition protein synthesis eukaryotic cells, and pentamer ligand B subunits (StxB) bind to globotria(tetra)osylceramide receptors (Gb3/Gb4) on cell membrane. Shiga toxin-producing Escherichia coli strains (STEC) may produce Stx1 and/or Stx2 variants. Strains carrying are considered more virulent related majority outbreaks, besides being usually...
We report a general approach to engineering multivalent d-proteins with antibody-like activities in vivo. Mirror-image phage display and structure-guided design were utilized create d-protein that uses receptor mimicry antagonize vascular endothelial growth factor A (VEGF-A). Selections against the form of VEGF-A using phage-displayed libraries two different domain scaffolds yielded proteins bound distinct interaction sites on VEGF-A. X-ray crystal structures d-protein/VEGF-A complexes used...
Ebola virus (EBOV) is a highly pathogenic member of the Filoviridae family viruses that causes severe hemorrhagic fever. Infection proceeds through fusion host cell and viral membranes, process mediated by envelope glycoprotein (GP). Following endosomal uptake, key step in entry proteolytic cleavage GP cysteine proteases. Cleavage exposes binding site for receptor Niemann-Pick C1 (NPC1) may induce conformational changes leading to membrane fusion. However, precise details structural...
Maraviroc (MVC), a specific antagonist of CCR5 expressed on macrophages and activated T cells, may modulate inflammation be useful in patients with HIV infection. In this study we used nonhuman primates to examine the effect mechanism MVC alone or combination cyclosporine (CsA) prolong cardiac allograft survivals. an established rhesus monkey model, recipients treated plus CsA showed significantly prolonged survival heart allografts (>240 d, p < 0.001). These vivo results MVC/CsA group...
The ebolaviruses cause severe and rapidly progressing hemorrhagic fever. There are five ebolavirus species; although much is known about Zaire (EBOV) its neutralization by antibodies, little Sudan (SUDV), which emerging with increasing frequency. Here we describe monoclonal antibodies containing a human framework that potently inhibit infection SUDV protect mice from lethal challenge. murine antibody 16F6, binds the envelope glycoprotein (GP), served as starting point for design. Sequence...
Abstract Harnessing complement-mediated cytotoxicity by therapeutic antibodies has been limited because of dependency on size and density antigen, structural constraints resulting from orientation antibody binding, blockade complement activation inhibitors expressed target cells. We developed a modular bispecific platform that directs the complement-initiating protein C1q to cells, increases local deposition induces against antigens with wide-range expression. The broad utility this approach...
The spread of COVID-19 has been exacerbated by the emergence variants concern (VoC). Many VoC contain mutations in spike protein (S-protein) and are implicated infection response to therapeutics. Bivalent neutralizing antibodies (nAbs) targeting S-protein receptor-binding domain (RBD) promising therapeutics for COVID-19, but they limited low potency vulnerability RBD VoC. To address these issues, we used naïve phage-displayed peptide libraries isolate optimize 16-residue peptides that bind...
Teilweise fluorierte Binol-Gerüste (siehe Bild) sind die Grundlage vielseitiger helical-chiraler Materialien. Unterschiedliche Fluorierungsmuster führen zu einem deutlich abweichenden Kristallpackungsverhalten, was Entwicklung neuartiger Materialien für Anwendungen von der Halbleitersynthese bis hin zur Katalyse erleichtern sollte. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2001/2008/z801991_s.pdf or from author. Please note: The...
Max-E47 is a designed hybrid protein comprising the Max DNA-binding basic region and E47 HLH dimerization subdomain. In yeast one-hybrid system (Y1H), shows strong transcriptional activation from E-box site, 5′-CACGTG, targeted by Myc/Max/Mad network of transcription factors; two mutants, Max-E47Y Max-E47YF, activate more weakly in Y1H. Quantitative fluorescence anisotropy titrations to gain free energies protein:DNA binding gave low nanomolar Kd values for native MaxbHLHZ, Max-E47, Y YF...
Polyomavirus JC (JCV) is the causative agent of progressive multifocal leukoencephalopathy (PML), a rare and frequently fatal brain disease that afflicts small fraction immune-compromised population, including those affected by AIDS transplantation recipients on immunosuppressive drug therapy. Currently there no specific therapy for PML. The major capsid viral protein 1 (VP1) involved in binding to sialic acid cell receptors believed be key player pathogenesis. PML-specific mutations JCV VP1...