A5103049789- Protein purification and stability
- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Microfluidic and Capillary Electrophoresis Applications
- Biosimilars and Bioanalytical Methods
- Advanced Proteomics Techniques and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Analytical Chemistry and Chromatography
- Biosensors and Analytical Detection
- Synthetic Organic Chemistry Methods
- Virus-based gene therapy research
- Surface Modification and Superhydrophobicity
- Microbial Natural Products and Biosynthesis
- Mass Spectrometry Techniques and Applications
- Neonatal Respiratory Health Research
- Peptidase Inhibition and Analysis
- Marine Sponges and Natural Products
- RNA and protein synthesis mechanisms
- Cancer, Hypoxia, and Metabolism
- Chemical Synthesis and Analysis
- HER2/EGFR in Cancer Research
- Click Chemistry and Applications
- Thermal and Kinetic Analysis
- Electrochemical Analysis and Applications
- Zeolite Catalysis and Synthesis
Regeneron (United States)
2017-2025
Zhongda Hospital Southeast University
2017-2022
University of Iowa
2016-2017
Southeast University
2017
Chinese Academy of Sciences
1994-2014
Shanghai Institute of Organic Chemistry
2014
UNSW Sydney
2013
Materials Science & Engineering
2000-2013
University of California, Riverside
2007-2009
The University of Texas at Arlington
1998-2003
Mesenchymal stem cells (MSCs) derived from bone marrow have potent stabilizing effects for the treatment of acute respiratory distress syndrome (ARDS). However, low efficiency and survival in MSC homing to injured lung tissue remains be solved. Therefore, aim this study was assess whether large intergenic noncoding RNA (LincRNA)-p21 promote migration capacity through hypoxic preconditioning vitro.MSCs were cultured divided into normoxia culture group (20% O2) hypoxia (1% O2). To determine...
Liquid chromatography-mass spectrometry has been widely implemented as a powerful tool for providing in-depth characterization of nucleic acid therapeutic modalities, such anti-sense oligonucleotides and small interfering RNAs (siRNAs). In this study, we developed generic hydrophilic interaction liquid chromatography (HILIC) hyphenated with tandem mass method in the absence ion-pairing reagents demonstrated its capability an attractive robust alternative oligonucleotide siRNA analysis. HILIC...
This paper describes an indium tin oxide (ITO) electrode-based Ru(bpy)3(2+) electrochemiluminecence (ECL) detector for a microchip capillary electrophoresis (CE). The CE-ECL system described in this article consists of poly(dimethylsiloxane) (PDMS) layer containing separation and injection channels electrode plate with ITO fabricated by photolithographic method. PDMS was reversibly bound to the plate, which greatly simplified alignment channel working enhanced photon-capturing efficiency. In...
Therapeutic proteins including monoclonal antibodies (mAbs) are usually produced in engineered host cell lines that also produce thousands of endogenous at varying levels. A critical aspect the development biotherapeutics manufacturing processes is removal these (HCP) to appropriate levels order minimize risk patient safety and drug efficacy. During process associated analytical characterization, mass spectrometry (MS) has become an increasingly popular tool for HCP analysis due its ability...
Charge heterogeneity is an important quality attribute of therapeutic antibodies, and a detailed understanding charge arising from post-translational modifications (PTMs) required by regulatory agencies during drug development. Among antibody therapeutics, the bispecific with two distinct Fab domains targeting antigens provides additional complexity to profile. In this study, variant species were enriched three antibodies (bsAbs) each containing one anti-CD3 binding arm designed differential...
The 18th Workshop on Recent Issues in Bioanalysis (18th WRIB) took place San Antonio, TX, USA May 6-10, 2024. Over 1100 professionals representing pharma/biotech companies, CROs, and multiple regulatory agencies convened to actively discuss the most current topics of interest bioanalysis. WRIB included 3 Main Workshops 7 Specialized that together spanned 1 week allow an exhaustive thorough coverage all major issues bioanalysis biomarkers, immunogenicity, gene therapy, cell therapy...
Adeno-associated viruses (AAVs) are non-enveloped, single-stranded DNA that have recently emerged as an attractive vector for delivering genetic materials to hosts gene therapy applications. Due their ability transduce a wide range of species and tissues in vivo, low risk immunotoxicity, mild innate adaptive immune responses, AAVs currently used research clinical studies monotherapy or with other biomolecules perform editing, replacement, addition, silencing. As new complex therapeutic...
The 15th edition of the Workshop on Recent Issues in Bioanalysis (15th WRIB) was held 27 September to 1 October 2021. Even with a last-minute move from in-person virtual, an overwhelmingly high number nearly 900 professionals representing pharma and biotech companies, contract research organizations (CROs), multiple regulatory agencies still eagerly convened actively discuss most current topics interest bioanalysis. WRIB included 3 Main Workshops 7 Specialized that together spanned week...
Charge heterogeneity is inherent to all therapeutic antibodies and arises from post-translational modifications (PTMs) and/or protein degradation events that may occur during manufacturing. Among antibodies, the bispecific antibody (bsAb) containing two unique Fab arms directed against different targets presents an additional layer of complexity charge profile. In context a bsAb, single domain-specific PTM within one domains be sufficient compromise target binding could potentially impact...
Abstract Background: Capillary electrophoresis (CE) with tris(2,2′-bipyridyl)ruthenium(II) [Ru(bpy)32+]-electrogenerated chemiluminescence (ECL) detection is a promising method for clinical analysis. In this study, combining CE Ru(bpy)32+ ECL (CE-ECL) that can be applied to amine-containing species was developed, and the performance of CE-ECL as quantitative determination sulpiride in human plasma or urine evaluated. Methods: Sulpiride separated by capillary zone uncoated fused-silica...
DNA-binding proteins are very important constituents of proteomes all species and play crucial roles in transcription, DNA replication, recombination, repair, other activities associated with DNA. Although a number have been identified, many involved gene regulation repair likely still unknown because their dynamic and/or weak interactions In this report, we described an approach for the comprehensive identification vivo formaldehyde cross-linking LC-MS/MS. could be purified via isolation...
Tungsten trioxide (WO3) thin films were synthesised by electrodeposition using peroxotungtic acid as the precursor electrolyte solution for use photoanodes in a photoelectrochemical cell solar hydrogen applications. The coated at deposition potentials varying from 0.30 to 0.90 V versus Ag/AgCl order study effect of potential on mineralogical, morphological, optical, and properties nanoparticulate films. composed monoclinic WO3, degree crystallinity preferred orientation orthogonal planes...
Abstract Great interest has been shown in mesenchymal stem cell (MSC) therapy a wide variety of clinical domains. However, the therapeutic efficiency depends on proliferation and migration MSCs. Chemokine receptors are involved regulating to specific organs MSCs different microenvironments. CXC receptor seven (CXCR7), newly discovered ligand 12 (CXCL12) receptor, organ specificity for tumour migration. We hypothesized that CXCR7 expression affects In present study, we constructed long-term...
Chinese hamster ovary (CHO) cell lines are grown in cultures with varying asparagine and glutamine concentrations, but further study is needed to characterize the interplay between these amino acids. By following 13 C-glucose, C-glutamine, C-asparagine tracers using metabolic flux analysis (MFA), CHO metabolism was characterized an industrially relevant fed-batch process under supplemented low conditions during early late exponential growth. For both MFA revealed glucose as primary carbon...
Abstract Adeno‐associated virus (AAV), a widely used gene therapy vector, is small, nonenveloped that contains single‐stranded DNA genome with maximum length of 4.7 kb. Despite extensive biophysical and structural characterization, many aspects AAV functions remain elusive. This knowledge gap primarily due to lack structurally resolved dynamic information the absence coverage functionally critical segments on capsid. Here, we developed protocol study dynamics by hydrogen‐deuterium exchange...
Tramadol and lidocaine, used as analgesic local anesthetic in surgery, are partly excreted by kidney. For the first time, we developed a simple sensitive method, based on capillary electrophoresis with electrochemiluminescence (ECL) detection end column mode without joint to monitor tramadol lidocaine urine. To eliminate influence of ionic strength urine sample, analytes were extracted ether. Tripropylamine (TPA) was internal standard. The recoveries between 94% 97% at different levels....
Plasma membrane proteins play critical roles in cell-to-cell recognition, signal transduction and material transport. Because of their accessibility, constitute the major targets for protein-based drugs. Here, we described an approach, which included stable isotope labeling by amino acids cell culture (SILAC), surface biotinylation, affinity peptide purification LC-MS/MS identification quantification proteins. We applied strategy quantitative analysis expressed a pair human melanoma lines,...
Abstract Background: Pulmonary microvascular endothelial cells (PMVECs) were not complex, and the barrier was destroyed in pathogenesis progress of acute lung injury (ALI)/acute respiratory distress syndrome (ARDS). Previous studies have demonstrated that hepatocyte growth factor (HGF), which secreted by bone marrow mesenchymal stem cells, could decrease apoptosis. We investigated whether mTOR/STAT3 signaling acted HGF protective effects against oxidative stress mitochondria-dependent...
A new setup to couple capillary electrophoresis (CE) with electrochemiluminescence (ECL) detection is described in which the electrical connection of CE achieved through a porous section at distance 7 mm from outlet. Because wall allowed current pass and there was no electric field gradient beyond that section, influence high-voltage on ECL procedure eliminated. The formed by etching hydrofluoric acid after only one side circumference 2−3 polyimide coating removed, while keeping other part...
A simultaneous electrochemical (EC) and electrochemiluminescence (ECL) detection scheme was introduced to both microchip conventional capillary electrophoresis (CE). In this dual scheme, tris(2,2'-bipyridyl)ruthenium(II) (Ru(bpy)32+) used as an ECL reagent well a catalyst (in the formation of Ru(bpy)33+) for EC detection. Ru(bpy)32+-ECL process, Ru(bpy)33+ generated then reacted with analytes resulting in emission great current enhancement due catalysis Ru(bpy)33+. The response signals were...
Host cell proteins (HCPs) are process-related impurities expressed by the host cells used for production of therapeutic proteins. Although an extensive purification process removes most HCPs, residual HCPs commonly present in protein therapeutics. If not well-controlled, certain may be product, impacting drug stability, and potentially affecting product safety leading to risks patients. Therefore, as a critical quality attribute, levels must closely monitored during development determined...
Many methods using liquid chromatography-mass spectrometry (LC-MS) have been established for identifying residual host cell proteins (HCPs) to aid in the process development and quality control of therapeutic proteins. However, use MS-based techniques adeno-associated virus (AAV) is still its infancy, with few reported minimal information available on potentially problematic HCPs. In this study, we developed a highly sensitive effective differential digestion method profile HCPs AAV. Unlike...