A5083584210- Biosimilars and Bioanalytical Methods
- Monoclonal and Polyclonal Antibodies Research
- Crystallization and Solubility Studies
- Biosensors and Analytical Detection
- Drug Transport and Resistance Mechanisms
- X-ray Diffraction in Crystallography
- Viral Infectious Diseases and Gene Expression in Insects
- Melanoma and MAPK Pathways
- Diet and metabolism studies
- Synthesis of Tetrazole Derivatives
- CAR-T cell therapy research
- Lysosomal Storage Disorders Research
- Synthesis and biological activity
- Amino Acid Enzymes and Metabolism
- Pharmacological Effects and Toxicity Studies
- Pharmacogenetics and Drug Metabolism
- Advanced Biosensing Techniques and Applications
- HER2/EGFR in Cancer Research
- Multiple Myeloma Research and Treatments
- Metabolism and Genetic Disorders
- Cancer Treatment and Pharmacology
- Epilepsy research and treatment
- CRISPR and Genetic Engineering
- RNA regulation and disease
- Advanced Optimization Algorithms Research
Takeda (United States)
2018-2025
Takeda (Japan)
2010-2024
AstraZeneca (United States)
2021-2023
Tokyo Institute of Technology
2008
SparsePOP is a Matlab implementation of the sparse semidefinite programming (SDP) relaxation method for approximating global optimal solution polynomial optimization problem (POP) proposed by Waki et al. [2006]. The SDP exploits structure polynomials in POPs when applying “a hierarchy LMI relaxations increasing dimensions” Lasserre efficiency to approximate solutions thus increased, and larger-scale can be handled.
The 17
The formation of antidrug antibodies (ADAs) against antibody-drug conjugates (ADCs) can trigger a humoral immune response and change drug exposure. Although the immunogenicity assessment an ADC in nonclinical nonhuman primates may not directly translate to potential humans, ADA assay facilitates understanding pharmacokinetic profiles biotherapeutics. human IgG4 monoclonal antibody-based was suspected cynomolgus monkey serum after intravenous administration at 1.5 mg/kg. However, conventional...
The 18th Workshop on Recent Issues in Bioanalysis (18th WRIB) took place San Antonio, TX, USA May 6-10, 2024. Over 1100 professionals representing pharma/biotech companies, CROs, and multiple regulatory agencies convened to actively discuss the most current topics of interest bioanalysis. WRIB included 3 Main Workshops 7 Specialized that together spanned 1 week allow an exhaustive thorough coverage all major issues bioanalysis biomarkers, immunogenicity, gene therapy, cell therapy...
The 15th edition of the Workshop on Recent Issues in Bioanalysis (15th WRIB) was held 27 September to 1 October 2021. Even with a last-minute move from in-person virtual, an overwhelmingly high number nearly 900 professionals representing pharma and biotech companies, contract research organizations (CROs), multiple regulatory agencies still eagerly convened actively discuss most current topics interest bioanalysis. WRIB included 3 Main Workshops 7 Specialized that together spanned week...
The magnitude of P-glycoprotein [(P-gp)/multidrug resistance protein 1 (MDR1)]-mediated drug-drug interaction (DDI) at the blood-brain barrier (BBB) in rats was estimated by vitro-in vivo correlation (IVIVC). In vitro studies, rat Mdr1a-expressing LLC-PK1 cells were examined for evaluation P-gp inhibitory activity using digoxin as a probe substrate. <i>K</i><sub>i</sub> value calculated modified corrected flux ratio that reflects function. with or without inhibitors administered to constant...
Characterizing in vivo cellular kinetics and biodistribution of chimeric antigen receptor T (CAR-T) cells is critical for toxicity assessment, nonclinical clinical efficacy studies. To date, the standardized assay to characterize CAR-T cell distribution, expansion, contraction, persistence profiles not readily available. overcome this limitation increase comparability among studies, we have established a universal protocol analysis. We duplexing ddPCR transgene reference gene normalize...
The 2022 16th Workshop on Recent Issues in Bioanalysis (WRIB) took place Atlanta, GA, USA September 26-30, 2022. Over 1000 professionals representing pharma/biotech companies, CROs, and multiple regulatory agencies convened to actively discuss the most current topics of interest bioanalysis. WRIB included 3 Main Workshops 7 Specialized that together spanned 1 week order allow exhaustive thorough coverage all major issues bioanalysis, biomarkers, immunogenicity, gene therapy, cell therapy...
In our pursuit of developing a novel, potent, and selective cell division cycle 7 (Cdc7) inhibitor, we optimized the previously reported thieno[3,2-d]pyrimidinone analogue I showing time-dependent Cdc7 kinase inhibition slow dissociation kinetics. These medicinal chemistry efforts led to identification compound 3d, which exhibited potent cellular activity, excellent selectivity, antitumor efficacy in COLO205 xenograft mouse model. However, issue formaldehyde adduct formation emerged during...
To date, the in vitro–in vivo correlation (IVIVC) of P-glycoprotein (P-gp)–mediated drug-drug interaction (DDI) at blood-brain barrier (BBB) rats indicated that cutoff value to significantly affect brain penetration digoxin was [I<sub>,unbound</sub>/<i>K</i><sub>i</sub>] 1, where I<sub>,unbound</sub> is unbound plasma concentration P-gp inhibitors. On basis IVIVC rats, we speculated clinically used inhibitors do not cause DDI human BBB, because none compounds studied...
The integration of ligand-binding assay and LC–MS/MS (immunocapture-LC–MS) has unleashed the combined advantages both powerful techniques for addressing ever increasing bioanalytical challenges biotherapeutics biomarker assays. highly specific, selective sensitive characteristics immunocapture-LC–MS-based assays have enabled determination biomarkers in biomatrices with ease method development, less requirements on key reagents as well structural specificity endogenous engineered...
We developed a highly sensitive and specific high-performance liquid chromatography with tandem mass spectrometry method an atmospheric pressure chemical ionization interface to determine 24S-hydroxycholesterol, major metabolite of cholesterol formed by cytochrome P450 family 46A1, in human plasma without any derivatization step. Phosphate buffered saline including 1% Tween 80 was used as the surrogate matrix for preparation calibration curves quality control samples. The saponification...
Abstract Fabry disease, an X-linked lysosomal storage disorder caused by galactosidase alpha (GLA) gene mutations, exhibits diverse clinical manifestations, and poses significant diagnostic challenges. Early diagnosis treatment are crucial for improved patient outcomes, pressing the need reliable biomarkers. In this study, we aimed to identify miRNA candidates as potential biomarkers disease using KingFisher™ automated isolation method NanoString nCounter® detection assay. Clinical serum...
Characterization of pharmacokinetic (PK) properties and target tissue distribution therapeutic fusion proteins (TFPs) are critical in supporting vivo efficacy. We evaluated the profile an investigational TFP consisting human immunoglobulin G4 fused to modified interferon alpha by orthogonal bioanalytical assays applied minimal physiologically based (PBPK) modeling characterize pharmacokinetics mouse. The conventional ligand binding assay (LBA), immunocapture-liquid chromatography/tandem mass...
For targeted therapies, immunocapture-liquid chromatography mass spectrometry (IC-LC/MS) technology has become an important tool for determination of both drug exposures, target antigen densities, and engagement in the systemic circulation and/or total tissue homogenates. Although information collected from homogenates is useful establishing correlations exposure with pharmacodynamic response efficacy a therapy, measurement at cell plasma membrane within preferred, since it primary action...
We identified a lead series of p38 mitogen-activated protein kinase inhibitors using structure-based design strategy from high-throughput screening hit compound 1. X-ray crystallography 1 with the showed an infrequent flip peptide bond between Met109 and Gly110, which was considered to high selectivity. Our conduct scaffold transformation maintenance hydrogen interactions flipped hinge backbone enzyme. In accordance this strategy, we focused on identify imidazo[4,5-b]pyridin-2-one...
Fabry disease, an X-linked lysosomal storage disorder caused by galactosidase α (GLA) gene mutations, exhibits diverse clinical manifestations, and poses significant diagnostic challenges. Early diagnosis treatment are crucial for improved patient outcomes, pressing the need reliable biomarkers. In this study, we aimed to identify miRNA candidates as potential biomarkers disease using KingFisher™ automated isolation method NanoString nCounter® detection assay. Clinical serum samples were...