A5013341831- Hearing, Cochlea, Tinnitus, Genetics
- Acoustic Wave Phenomena Research
- RNA and protein synthesis mechanisms
- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Lipid Membrane Structure and Behavior
- Advanced Fluorescence Microscopy Techniques
- Plant and Biological Electrophysiology Studies
- Ion Channels and Receptors
- Protein Structure and Dynamics
- Electron Spin Resonance Studies
- Hearing Loss and Rehabilitation
- Amino Acid Enzymes and Metabolism
- Metabolism and Genetic Disorders
- Postharvest Quality and Shelf Life Management
- Advanced biosensing and bioanalysis techniques
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Erythrocyte Function and Pathophysiology
- Birth, Development, and Health
- Bioactive Compounds and Antitumor Agents
- Photoreceptor and optogenetics research
- Connexins and lens biology
- Prenatal Substance Exposure Effects
- Cytokine Signaling Pathways and Interactions
- Bacterial Genetics and Biotechnology
Harvard University
2016-2024
Howard Hughes Medical Institute
2016-2018
Cornell University
2013-2018
Weill Cornell Medicine
2018
The transmembrane (TM) channel-like 1 (TMC1) and TMC2 proteins play a central role in auditory transduction, forming ion channels that convert sound into electrical signals. However, the molecular mechanism of their gating remains unknown. Here, using predicted structural models as guide, we probed effects 12 mutations on mechanical transduction currents native hair cells Tmc1/2 -null mice expressing virally introduced TMC1 variants. Whole-cell electrophysiological recordings revealed within...
Our ability to hear and maintain balance relies on the proper functioning of inner ear sensory hair cells, which translate mechanical stimuli into electrical signals via mechano-electrical transducer (MET) channels, composed TMC1/2 proteins. However, therapeutic use ototoxic drugs, such as aminoglycosides cisplatin, can enter cells through MET often leads profound auditory vestibular dysfunction. Despite extensive research otoprotective compounds targeting our understanding how small...
The brain morphometry of 21 children, who were followed from birth and underwent structural magnetic resonance imaging at 8-10 years, was studied. This cohort included 11 children with prenatal cocaine exposure (CE) 10 noncocaine-exposed (NCE). We compared the CE versus NCE groups using FreeSurfer to automatically segment quantify volume individual structures. In addition, we created a pediatric atlas specifically for this population demonstrate enhanced accuracy approach. found an overall...
TRPA1 (transient-receptor-potential-related ion channel with ankyrin domains) is a direct receptor or indirect effector for wide variety of nociceptive signals, and thus compelling target development analgesic pharmaceuticals such as blockers. Recently, the structure was reported, providing insights into assembly pore architecture. Here we report whole-cell single-channel current recordings wild-type human well bearing point mutations key charged residues in outer pore. These measurements...
Membrane proteins such as ion channels and transporters are frequently homomeric. The homomeric nature raises important questions regarding coupling between subunits complicates the application of techniques FRET or DEER spectroscopy. These challenges can be overcome if a protein independently modified for functional spectroscopic studies. Here, we describe general approach in vitro assembly that used generation heteromeric variants membrane proteins. We establish using GltPh, glutamate...
Advances in single‐molecule imaging and fluorescence technologies now enable direct insights into functional dynamic processes diverse families of integral membrane proteins. These data pave the way for quantitative descriptions function from intuitive perspective time‐dependent changes structure. Challenges on this road will be discussed context recent advances field.
Abstract The trans m embrane c hannel-like (TMC) 1 and 2 proteins play a central role in auditory transduction, forming ion channels that convert sound into electrical signals. However, the molecular mechanism of their gating remains unknown. Here, using predicted structural models as guide, we probed effects twelve mutations on mechanical transduction currents native hair cells Tmc1/2 -null mice expressing virally introduced TMC1 variants. Whole-cell electrophysiological recordings revealed...