A5082192714- Advanced biosensing and bioanalysis techniques
- Extracellular vesicles in disease
- Biosensors and Analytical Detection
- CRISPR and Genetic Engineering
- Advanced Biosensing Techniques and Applications
- Advanced Nanomaterials in Catalysis
- Nanocluster Synthesis and Applications
- Gold and Silver Nanoparticles Synthesis and Applications
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- SARS-CoV-2 detection and testing
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- Electrochemical Analysis and Applications
- Nanoplatforms for cancer theranostics
- Innovation and Socioeconomic Development
- Gene expression and cancer classification
- Electrochemical sensors and biosensors
- Cellular transport and secretion
- Ferroptosis and cancer prognosis
- Dye analysis and toxicity
- Carbon and Quantum Dots Applications
- Botulinum Toxin and Related Neurological Disorders
- Endoplasmic Reticulum Stress and Disease
- Anodic Oxide Films and Nanostructures
Konkuk University
2019-2024
Konkuk University Medical Center
2019
Government of the Republic of Korea
2019
DNA-templated copper nanoclusters (CuNCs) have limited applications because of their low fluorescence stability (several tens minutes). In this study, we prepared CuNCs with improved temporal by introducing fructose into the CuNC synthesis process and optimizing reaction conditions. The inclusion increased operating lifetime approximately 5200-fold from 30 min to 108 days against heat, acids, bases compared synthesized under original addition, signal was maintained for a significantly longer...
A strategy combining the primer exchange reaction with transcription isothermal amplification, termed PER-Trap, is developed as a sensitive biomolecular assay.
Loop-mediated isothermal amplification (LAMP) is one of the most widely used technologies in molecular diagnostics. However, LAMP operates at a high temperature 65 °C; thus, operating lower desirable to maximize its usefulness for on-site diagnosis. In this study, we propose new version LAMP, termed low-temperature which physiological 37 °C. Low-temperature differs from conventional °C terms concentrations MgSO4 and deoxyribonucleoside triphosphates (dNTPs), as well lengths DNA probes, are...
Colorectal cancer (CRC) as the second leading cause of global deaths poses critical challenges in clinical settings. Cancer-derived small extracellular vesicles (sEVs), which are secreted by cells, have been shown to mediate tumor development, invasion, and even metastasis, thus received increasing attention for development diagnostic or therapeutic platforms. In present study, sEV-targeted systematic evolution ligands exponential enrichment (E-SELEX) is developed generate a high-quality...
With the increasing incidence of colorectal cancer (CRC), its accurate diagnosis is critical and in high demand. However, conventional methods are not ideal due to invasiveness low accuracy. Herein, we aimed identify efficient CRC mRNA markers a non-invasive manner using CRC-derived extracellular vesicles (EVs). The expression levels EV mRNAs from cell lines were compared with those normal line quantitative polymerase chain reaction. Eight evaluated plasma EVs patients healthy controls....
Biological macromolecules, such as DNA, RNA, and proteins in living organisms, form an intricate network that plays a key role many biological processes. Many attempts have been made to build new networks by connecting non-communicable with mediators, especially using antibodies. In this study, we devised aptamer-based switching system enables communication between non-interacting proteins. As proof of concept, two proteins, Cas13a T7 RNA polymerase (T7 RNAP), were rationally connected...
Astaxanthin (AST) exhibits potent antioxidant and anti-inflammatory activities but poor stability biological efficacy, which limit its application in the food medical industries. In present study, a new strategy was proposed to enhance of AST using fetal bovine serum-derived extracellular vesicles (EVs). Saponin-assisted incubation used load owing high encapsulation efficiency loading capacity. AST-incorporated EVs (EV-ASTs) maintained their original EV morphology showed at 4 °C, 25 37 °C...
In recent years, fluorescent metal nanoclusters have been used to develop bioimaging and sensing technology. Notably, protein-templated gold (AuNCs) are attracting interest due their excellent fluorescence properties biocompatibility. Herein, we an exosome template synthesize AuNCs in eco-friendly manner that required neither harsh conditions nor toxic chemicals. Specifically, a neutral (pH 7) alkaline 11.5) pH two different exosome-based (exo-AuNCs) with independent blue red emission. Using...