A5079289762- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Extracellular vesicles in disease
- CRISPR and Genetic Engineering
- MicroRNA in disease regulation
- Nanocluster Synthesis and Applications
- Advanced Biosensing Techniques and Applications
- RNA Interference and Gene Delivery
- Advanced Nanomaterials in Catalysis
- SARS-CoV-2 detection and testing
- RNA and protein synthesis mechanisms
- Gold and Silver Nanoparticles Synthesis and Applications
- Bacteriophages and microbial interactions
- Cancer-related molecular mechanisms research
- Nanoplatforms for cancer theranostics
- Electrochemical Analysis and Applications
- Carbon and Quantum Dots Applications
- Electrochemical sensors and biosensors
- Innovation and Socioeconomic Development
- Electrospun Nanofibers in Biomedical Applications
- Gene expression and cancer classification
- RNA regulation and disease
- Vitamin C and Antioxidants Research
- Dye analysis and toxicity
- Lightning and Electromagnetic Phenomena
Konkuk University
2019-2024
Konkuk University Medical Center
2019-2021
Government of the Republic of Korea
2019-2021
Hyundai Motor Group (South Korea)
2017
DNA-templated copper nanoclusters (CuNCs) have limited applications because of their low fluorescence stability (several tens minutes). In this study, we prepared CuNCs with improved temporal by introducing fructose into the CuNC synthesis process and optimizing reaction conditions. The inclusion increased operating lifetime approximately 5200-fold from 30 min to 108 days against heat, acids, bases compared synthesized under original addition, signal was maintained for a significantly longer...
A strategy combining the primer exchange reaction with transcription isothermal amplification, termed PER-Trap, is developed as a sensitive biomolecular assay.
Loop-mediated isothermal amplification (LAMP) is one of the most widely used technologies in molecular diagnostics. However, LAMP operates at a high temperature 65 °C; thus, operating lower desirable to maximize its usefulness for on-site diagnosis. In this study, we propose new version LAMP, termed low-temperature which physiological 37 °C. Low-temperature differs from conventional °C terms concentrations MgSO4 and deoxyribonucleoside triphosphates (dNTPs), as well lengths DNA probes, are...
Colorectal cancer (CRC) as the second leading cause of global deaths poses critical challenges in clinical settings. Cancer-derived small extracellular vesicles (sEVs), which are secreted by cells, have been shown to mediate tumor development, invasion, and even metastasis, thus received increasing attention for development diagnostic or therapeutic platforms. In present study, sEV-targeted systematic evolution ligands exponential enrichment (E-SELEX) is developed generate a high-quality...
Biological macromolecules, such as DNA, RNA, and proteins in living organisms, form an intricate network that plays a key role many biological processes. Many attempts have been made to build new networks by connecting non-communicable with mediators, especially using antibodies. In this study, we devised aptamer-based switching system enables communication between non-interacting proteins. As proof of concept, two proteins, Cas13a T7 RNA polymerase (T7 RNAP), were rationally connected...
Bisphenol A (BPA) is used in a wide variety of consumer products owing to its beneficial properties optical clarity, shatter resistance, and heat resistance. However, leached BPA has been shown disturb the endocrine system could cause cancer even at low concentrations, which led public concern. To reduce toxic effects caused by BPA, it important monitor levels presence simple, rapid, on-site manner. Here, we propose new colorimetric strategy for simple rapid detection employing DNA aptamer,...
Astaxanthin (AST) exhibits potent antioxidant and anti-inflammatory activities but poor stability biological efficacy, which limit its application in the food medical industries. In present study, a new strategy was proposed to enhance of AST using fetal bovine serum-derived extracellular vesicles (EVs). Saponin-assisted incubation used load owing high encapsulation efficiency loading capacity. AST-incorporated EVs (EV-ASTs) maintained their original EV morphology showed at 4 °C, 25 37 °C...
Astaxanthin is a strong antioxidant, but the effect of esterification on its biological activities remains unclear. Here, we chemically synthesized three forms astaxanthin (nonesterified (Ast-N), monoesterified (Ast-mE), and diesterified (Ast-dE) forms) using esterified (Ast-E) in natural extract from Haematococcus pluvialis characterized them by spectrophotometry high-performance liquid chromatography (HPLC). Additionally, antioxidant anti-inflammatory samples containing at different ratios...
DNA-templated copper nanoparticles function as a probe for fluorescence resonance energy transfer, allowing the ratiometric detection of miRNAs.