A5079857401- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- Genomics and Phylogenetic Studies
- Tea Polyphenols and Effects
- Enzyme Production and Characterization
- Fermentation and Sensory Analysis
- Vibrio bacteria research studies
- Metabolomics and Mass Spectrometry Studies
- Phytase and its Applications
- Protein Hydrolysis and Bioactive Peptides
- Plant Pathogenic Bacteria Studies
- Microbial Metabolites in Food Biotechnology
- Plant nutrient uptake and metabolism
- Ginkgo biloba and Cashew Applications
- Food Quality and Safety Studies
- Legume Nitrogen Fixing Symbiosis
- Microbial Natural Products and Biosynthesis
- Iron oxide chemistry and applications
- Bioinformatics and Genomic Networks
- Probiotics and Fermented Foods
- Diet, Metabolism, and Disease
- Plant and Fungal Species Descriptions
- Genetic diversity and population structure
- Burkholderia infections and melioidosis
Kansas State University
2017-2024
Jacobs Institute
2021-2024
University at Buffalo, State University of New York
2020-2022
Jadavpur University
2011-2016
National Dairy Research Institute
1964
Acinetobacter baumannii is a highly antibiotic-resistant bacterial pathogen for which novel therapeutic approaches are needed. Unfortunately, the drivers of virulence in A . remain uncertain. By comparing genomes among panel strains we identified specific gene variation capsule locus that correlated with altered virulence. While less virulent possessed intact gtr6 , hypervirulent clinical isolate contained spontaneous transposon insertion same gene, resulting loss branchpoint capsular...
Microbes synthesize and secrete siderophores, that bind solubilize precipitated or otherwise unavailable iron in their microenvironments. Gram (-) bacterial TonB-dependent outer membrane receptors capture the resulting ferric siderophores to begin uptake process. From similarity
Siderophores are iron-chelating molecules that solubilize Fe3+ for microbial utilization and facilitate colonization or infection of eukaryotes by liberating host iron bacterial uptake. By fluorescently labeling membrane receptors binding proteins, we created 20 sensors detect, discriminate, quantify apo- ferric siderophores. The sensor proteins originated from TonB-dependent ligand-gated porins (LGPs) Escherichia coli (Fiu, FepA, Cir, FhuA, IutA, BtuB), Klebsiella pneumoniae (IroN, FyuA),...
Gram-negative bacteria acquire ferric siderophores through TonB-dependent outer membrane transporters (TBDT). By fluorescence spectroscopic hgh-throughput screening (FLHTS), we identified inhibitors of enterobactin (FeEnt) uptake Escherichia coli FepA (EcoFepA). Among 165 found in a primary screen 17,441 compounds, evaluated 20 secondary tests: siderophore and colicin killing proton motive force-dependent lactose transport. Six hits inhibited activity all tests. Comparison their effects on...
A total of 230 cultures fungi and 43 bacteria, isolated from such sources as soil, butter, milk, were screened for their milk-clotting activity. The cultivated on semisolid media, the bacteria grown in milk media shake culture. Phytic acid, added calcium phytate, was found to stimulate production enzyme most bacterial isolates. Proteolytic activity invariably be associated with There considerable variation ratio two enzymes strain strain.
A total of 230 cultures fungi and 43 bacteria, isolated from such sources as soil, butter, milk, were screened for their milk-clotting activity. The cultivated on semisolid media, the bacteria grown in milk media shake culture. Phytic acid, added calcium phytate, was found to stimulate production enzyme most bacterial isolates. Proteolytic activity invariably be associated with There considerable variation ratio two enzymes strain strain.
Acinetobacter baumannii is a problematic pathogen due to its common expression of extensive drug resistance (XDR) and ability survive in the healthcare environment. These characteristics are mediated, part, by signal transduction system BfmR/BfmS. We previously demonstrated, antimicrobial sensitive clinical isolates, that BfmR conferred increased meropenem polymyxin E. In this study, potential mechanisms were informed, prior transcriptome analysis isolate AB307-0294, which identified porins...
This study reports the identification of a chitin deacetylase gene in Cryptococcus laurentii strain RY1 over‐expressing under nitrogen limitation by differential display. The up‐regulation took place robustly growing cells rather than starving quiescent autophagic cells. Quantitative Real Time‐PCR, enzyme activity cell lysate and wall analysis corroborated limitation. These results suggest might play significant role limiting growth RY1.
The Acetobacteraceae family of the class Alpha Proteobacteria is comprised high sugar and acid tolerant bacteria. Acetic Acid Bacteria are economically most significant group this because its association with food products like vinegar, wine etc. often hard to culture in laboratory conditions they also maintain very low abundances their natural habitats. Thus identification organisms such environments greatly dependent on modern tools molecular biology which require a thorough knowledge...
Enterococcus gallinarum strains isolated from some Nigerian fermented foods were found to produce bacteriocins. The bacteriocins had a broad spectrum of activity against both Gram-positive and negative bacteria. effects the bacteriocinogenic organ- isms on Staphylococcus aureus infections in rats evaluated. Sprague-Dawley infected with S. MTCC 737 treated E. T71 different concentrations W211 T71. infection caused significant upregulation aspartate aminotransferase alanine levels sera rats....
Summary: SSDNA Cutter v0.0 is a new in silico RFLP tool written C. It has significant utility studying population diversity as it the capability of restriction digestion group linear DNA se-quences and then segregate patterns into separate groups per similarity maps. The software an inbuilt database for 20 enzymes flexible so that user can add up to 100 more list. interface easy, simple interactive which enables obtain pattern are quite similar Operational Taxonomic Units obtained from...
Bacterial iron acquisition systems are potential targets for new antibiotics, because plays a central role in energy production, DNA synthesis, intermediate metabolism, nitrogen fixation, and oxygen detoxification. Bacteria actively acquire by secreting siderophores that chelate Fe 3+ , then transporting the ferric siderophore complexes. Pathogenic K. pneumoniae produces at least four siderophores: enterobactin, glycosylated yersiniabactin, aerobactin. Like all Gram‐negative species, obtains...