A5084277964- Prostate Cancer Treatment and Research
- Breast Cancer Treatment Studies
- Neurogenesis and neuroplasticity mechanisms
- Cancer, Hypoxia, and Metabolism
- HER2/EGFR in Cancer Research
- Estrogen and related hormone effects
- Cancer Diagnosis and Treatment
- Cancer Cells and Metastasis
- Extracellular vesicles in disease
- Prostate Cancer Diagnosis and Treatment
- Nitrogen and Sulfur Effects on Brassica
- Pluripotent Stem Cells Research
- Bone health and treatments
- Eicosanoids and Hypertension Pharmacology
- Renal Diseases and Glomerulopathies
- Cancer Genomics and Diagnostics
- Glioma Diagnosis and Treatment
- Cancer Treatment and Pharmacology
- Neurological Complications and Syndromes
- Angiogenesis and VEGF in Cancer
- 3D Printing in Biomedical Research
- Lipid metabolism and disorders
- Advanced Breast Cancer Therapies
- Chronic Myeloid Leukemia Treatments
- Microtubule and mitosis dynamics
Ospedale di Parma
2024
Azienda Socio Sanitaria Territoriale degli Spedali Civili di Brescia
2016-2022
University of Brescia
2016-2020
Brescia University
2019
Università degli Studi del Piemonte Orientale “Amedeo Avogadro”
2017
European Institute of Oncology
2017
Ipsen (France)
2017
Novartis (France)
2017
Istituti Ospitalieri di Cremona
2011-2014
Concordia University Wisconsin
2011-2013
Abstract Transformed stem cells have been isolated from some human cancers. We report that, unlike other brain cancers, the lethal glioblastoma multiforme contains neural precursors endowed with all of critical features expected cells. Similar, yet not identical, to their normal cell counterpart, these emerge as unipotent (astroglial) in vivo and multipotent (neuronal-astroglial-oligodendroglial) culture. More importantly, can act tumor-founding down clonal level establish tumors that...
The objective of this study was to determine the optimal scheduling 2.5 mg daily letrozole in neoadjuvant breast cancer patients obtain pathological complete response (pathCR) and assess Ki-67 expression as an early predictor response. This single institution comprised 120 oestrogen receptor (ER)-positive postmenopausal women with primary (clinical stage ⩾T2, N0–1), from three sequential cohorts (cohort A 40, cohort B 40 C patients, respectively) based on different duration letrozole....
Detection of BRAFV600E within cell free tumor DNA (ctDNA) is emerging as a promising means to improve patients' stratification or enable BRAF inhibitor (BRAFi) therapeutic monitoring in minimally invasive manner. Here, we investigated whether extracellular vesicle-(EV)-associated-DNA (EV-DNA) has value an alternative source circulating BRAFV600E. To do so, identified clinical practice-compatible protocol for the isolation EV-DNA and assessed gene status on plasma samples from metastatic...
We evaluated the advantages and reliability of novel protocols for enrichment tumor extracellular vesicles (EVs), enabling a blood-based test noninvasive parallel profiling multiple androgen receptor (AR) gene alterations. Three clinically relevant AR variants related to response/resistance standard-of-care treatments (AR-V7 transcript, T878A point mutation amplification) were by digital PCR in 15 samples from patients affected Castration-Resistant Prostate Cancer (CRPC). Plasma was...
we isolated a novel gene that is selectively induced both in roots and shoots response to sulfur starvation. This encodes cytosolic, monomeric protein of 33 kD binds NADPH. The predicted polypeptide highly homologous ( > 70%) leguminous isoflavone reductases (IFRs), but the maize (IRL for reductase-like) belongs family proteins present variety plants. Anti-IRL antibodies specifically recognize IFR polypeptides, yet unable use various isoflavonoids as substrates. IRL expression correlated...
Recent observations have suggested that extensive culturing of adult neural stem cells (ANSCs) by exploiting the NeuroSphere assay might select for aggressive cell clones, endowed with neoplastic potential, overgrow rest native cells. However, a detailed study propensity ANSCs to transform has never been thoroughly undertaken. Here, we report first demonstration can be propagated in vitro over year, maintaining strikingly stable profile regard self-renewal, differentiation, growth factor...
We propose a pilot, prospective, translational study with the aim of identifying possible molecular markers underlying metastatic prostate cancer (PC) evolution use liquid biopsy. Twenty-eight castrate sensitive, oligometastatic PC patients undergoing bone and/or nodal stereotactic body radiotherapy (SBRT) were recruited. Peripheral blood samples collected before commencement SBRT, then they processed for circulating cell free DNA (cfDNA) extraction. Deep targeted sequencing was performed...
To understand global features of gene expression changes during in vitro neural differentiation, we carried out the microarray analysis embryonic stem cells (ESCs), embryonal carcinoma cells, and adult stem/progenitor (NS) cells. Expression profiling ESCs differentiation monolayer culture revealed three distinct phases: undifferentiated ESCs, primitive ectoderm-like progenitor Principal component (PC) that these were aligned on PC1 over course 6 days. This represents approximately 4,000...
DNA repair is a key determinant in the cellular response to therapy and tumor status could play an important role tailoring patient therapy. Our goal was evaluate mRNA of 13 genes involved different pathways (base excision, nucleotide homologous recombination, Fanconi anemia) paraffin embedded samples triple negative breast cancer (TNBC) compared luminal A (LABC). Most excision Anemia pathways, CHK1 gene were significantly less expressed TNBC than LABC. PARP1 levels higher In univariate...
Due to the discovery of their role in intra‑cellular communications, exosomes, which carry information specific cell origin, have garnered considerable attention cancer research. Moreover, there is evidence suggest possibility isolating different exosome sub‑populations based on target antigens at surface. Philadelphia chromosome‑positive (Ph+) chronic myeloid leukemia (CML) a clonal myeloproliferative neoplasia characterized by breakpoint cluster region‑proto‑oncogene 1 tyrosine‑protein...
To assess whether the combination of letrozole, metronomic cyclophosphamide and sorafenib (LCS) is well tolerated shows activity in primary breast cancer (BC). Thirteen oestrogen receptor-positive, postmenopausal, T2-4, N0-1 BC patients received LCS for 6 months. In these we examined pharmacokinetics cyclophosphamide, toxicity regimen, clinical response to therapy changes levels biologically relevant biomarkers. Adequate plasma concentrations were achieved when it was dosed with L+C. The...
Abstract Neural stem cells (NSCs) in the murine subventricular zone (SVZ) niche allow life‐long neurogenesis. During first postnatal month and throughout aging, decrease of neuroblasts rise astrocytes results diminished neurogenesis increased astrocyte:neuron ratio. Also, a different neurogenic activity characterizes SVZ periventricular region (LV, lateral ventricle) as compared to its rostral extension (RE). In order investigate whether what extent these physiological modifications may be...
Abstract Reliable procedures to induce neural commitment of totipotent undifferentiated embryonic stem (ES) cells have provided new tools for investigating the molecular mechanisms underlying cell fate choices. We extensively characterized developmental potential ES-induced obtained using an adaptation multistep induction protocol. evidence that ES-derived proliferating are endowed with properties such as extensive self-renewal capacity and single-cell multipotency. In differentiating...
Breast cancer is a heterogeneous disease. Predictive molecular markers are crucial in patient management, but the only recommended predictive biomarkers estrogen and progesterone receptors HER2. There many new targeted therapies, although target pathway expression readily analyzed on conventional pathology, dynamic response cannot be assessed no guarantee it has major driver role, even if mutated. Selecting therapies requires considering patient, characteristics of tumor, microenvironment...
The identification of germline mutations in familial leukemia predisposition genes by next generation sequencing is pivotal importance. Lately, some "blend pedigrees" characterized both solid and hematologic malignancies have been described. Some were recognized as related to this double predisposition, while the involvement others still a matter debate. ETV6 was associated with malignancies, particular myeloid recently described mutated also oncologic patients. No clear evidences its blend...