A5020365426- Chronic Myeloid Leukemia Treatments
- Bone Tissue Engineering Materials
- Periodontal Regeneration and Treatments
- Acute Myeloid Leukemia Research
- Mesenchymal stem cell research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Electrospun Nanofibers in Biomedical Applications
- Chronic Lymphocytic Leukemia Research
- CAR-T cell therapy research
- Hematopoietic Stem Cell Transplantation
- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Eosinophilic Disorders and Syndromes
- Extracellular vesicles in disease
- Virus-based gene therapy research
- Osteoarthritis Treatment and Mechanisms
- Cytomegalovirus and herpesvirus research
- Impact of Technology on Adolescents
- Lung Cancer Treatments and Mutations
- Acute Lymphoblastic Leukemia research
- Digital Mental Health Interventions
- Child Development and Digital Technology
- Reconstructive Surgery and Microvascular Techniques
- Mycobacterium research and diagnosis
- MicroRNA in disease regulation
University of Brescia
2019-2025
Azienda Socio Sanitaria Territoriale degli Spedali Civili di Brescia
2019-2025
Ospedale Vito Fazzi
2024
Brescia University
2019
University of Milan
2016-2017
Luigi Sacco Hospital
2014
Centre Hospitalier Universitaire de Liège
2014
Bone marrow and adipose tissue human mesenchymal stem cells were seeded in highly performing 3D gelatin–chitosan hybrid hydrogels of varying chitosan content the presence platelet lysate evaluated for their proliferation osteogenic differentiation. Both bone hydrogel 1 (chitosan 8.1%) or 2 14.9%) showed high levels viability (80%–90%), differentiation was significantly higher with compared to fetal bovine serum, particularly 1. Mineralization detected early, after 21 days culture, when used...
A Deep Molecular Response (DMR), defined as a BCR::ABL1 transcript at levels ≤ 0.01% by RT-qPCR, is the prerequisite for successful interruption of treatment among patients with Chronic Myeloid Leukemia (CML). However, approximately 50% in Treatment-Free Remission (TFR) studies had to resume therapy after their rose above 0.1% threshold. To improve detection sensitivity and accuracy, can be analyzed using digital PCR (dPCR). dPCR increases 10–100 fold; however, its ability better select TFR...
Motivated by the enormous potential of hydrogels in regenerative medicine, new biocompatible gelatin-based hybrid were developed through a green process using poly(ethylene glycol) diglycidyl ether as cross-linking agent, adding carrageenan and chitosan polysaccharides to network better mimic composition native extracellular matrix. Overall, show suitable structural stability, high porosity pore interconnectivity, good swellability, finally, biocompatibility. Their mechanical behavior,...
Problematic internet use (PIU) has been suggested as in need of further research with a view to being included disorder future Diagnostic and Statistical Manual (DSM) the American Psychiatric Association, but lack knowledge about impact cessation on physiological function remains major gap barrier PIU classification. One hundred forty-four participants were assessed for (blood pressure heart rate) psychological (mood state anxiety) before after an session. Individuals also completed...
Sinusoidal obstruction syndrome (SOS), also known as veno-occlusive disease (VOD), is a rare but potentially fatal complication following allogenic hematopoietic cell transplantation (allo-HCT). Timely identification of SOS/VOD to allow for prompt treatment critical, identifying VOD-predictive biomarker remains challenging. Given the pivotal role endothelial dysfunction in pathophysiology, CECinVOD study prospectively evaluated levels circulating cells (CECs) patients undergoing allo-HCT...
ABSTRACT Chronic Myeloid Leukemia (CML) is characterized by the BCR::ABL1 fusion gene, driving uncontrolled myeloid cell proliferation. Furthermore, metabolic dysregulation contributes to disease progression. Despite efficacy of tyrosine kinase inhibitors (TKIs), unresolved clinical needs persist, necessitating refined preclinical models. This study compared responses three commonly used CML lines (K562, LAMA84, KCL22) five TKIs (imatinib, nilotinib, dasatinib, bosutinib, ponatinib) and a...
Due to the discovery of their role in intra‑cellular communications, exosomes, which carry information specific cell origin, have garnered considerable attention cancer research. Moreover, there is evidence suggest possibility isolating different exosome sub‑populations based on target antigens at surface. Philadelphia chromosome‑positive (Ph+) chronic myeloid leukemia (CML) a clonal myeloproliferative neoplasia characterized by breakpoint cluster region‑proto‑oncogene 1 tyrosine‑protein...
A great promise for tissue engineering is represented by scaffolds that host stem cells during proliferation and differentiation simultaneously replace damaged while maintaining the main vital functions. In this paper, a novel process was adopted to develop composite with core-shell structure bone regeneration, in which core has function of temporary mechanical support, shell enhances biocompatibility provides bioactive properties. An interconnected porous safely obtained, avoiding solvents...
Selection of feasible hybrid-hydrogels for best chondrogenic differentiation human mesenchymal stromal cells (hMSCs) represents an important challenge in cartilage regeneration. In this study, three-dimensional hybrid hydrogels obtained by chemical crosslinking poly (ethylene glycol) diglycidyl ether (PEGDGE), gelatin (G) without or with chitosan (Ch) dextran (Dx) polysaccharides were developed. The hydrogels, namely G-PEG, G-PEG-Ch and G-PEG-Dx, prepared innovative, versatile cell-friendly...
Chimeric antigen receptor (CAR) T-cell therapy represents the most advanced immunotherapy against relapsed/refractory B cell malignancies. While cytokine release syndrome and immune effector cell-associated neurotoxicity are distinctive, known CAR acute adverse events, hematological toxicity has been increasingly reported. Cytopenia following treatment is attributed in cases to lymphodepletion regimens, bridging chemotherapy, or radiotherapy. However, when cytopenia becomes prolonged,...
Abstract Rational design and development of tailorable simple synthesis process remains a centerpiece investigational efforts toward engineering advanced hydrogels. In this study, green scalable approach is developed to formulate set gelatin‐based macroporous hybrid This consists four sequential steps starting from liquid‐phase pre‐crosslinking/grafting, unidirectional freezing, freeze‐drying, finally post‐curing process. The chemical crosslinking mainly involves between epoxy groups...
One of the main hurdles to improving scaffolds for regenerative medicine is development non-invasive methods monitor cell proliferation within three-dimensional environments. Recently, an electrical impedance-based approach has been identified as promising assays. A low-cost solution, easily integrable with multi-well plates, here presented. Sensors were developed using biocompatible carbon-based ink on foldable polyimide substrates by means a novel aerosol jet printing technique. The setup...
Cartilage tissue engineering remains problematic because no systems are able to induce signals that contribute native cartilage structure formation. Therefore, we tested the potentiality of gelatin-polyethylene glycol scaffolds containing three different concentrations chitosan (CH; 0%, 8%, and 16%) on chondrogenic differentiation human platelet lysate-expanded bone marrow mesenchymal stromal cells (hBM-MSCs). Typical (SOX9, collagen type 2, aggrecan), hypertrophic (collagen 10), fibrotic 1)...
Real-time quantitative PCR (RT-qPCR) is the gold standard to quantify BCR-ABL1 transcript for molecular response monitoring in chronic myeloid leukemia (CML) patients, and it plays a pivotal role clinical decision-making process, even if presents technical limits. Increasing data suggest that digital (dPCR) more accurate reliable than RT-qPCR CML minimal residual disease patients' selection treatment discontinuation. But what about identification of discontinuation failures? We present case...
Gelatin–dextran hydrogel scaffolds (G-PEG-Dx) were evaluated for their ability to activate the bone marrow human mesenchymal stromal cells (BM-hMSCs) towards mineralization. G-PEG-Dx1 and G-PEG-Dx2, with identical composition but different architecture, seeded BM-hMSCs in presence of fetal bovine serum or platelet lysate (hPL) without osteogenic medium. G-PEG-Dx1, characterized by a lower degree crosslinking larger pores, was able induce better cell colonization than G-PEG-Dx2. At day 28,...
Background: Disease relapse after allogeneic stem cell transplantation (allo-SCT) is the main challenge for curing acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS). We investigated overall survival (OS) allo-SCT according to different therapeutic approaches. Methods: analyzed 134 patients who relapsed performed between 2015 2021 at Saint-Antoine University Hospital, Paris Spedali Civili di Brescia, Brescia. Of these, 103 (77%) were treated, comprising 69/103 (67%) received...
Infectious complications are a significant cause of morbidity and mortality in patients undergoing allogeneic haematopoietic stem cell transplantation (Allo-SCT). The BATMO (Best-Antimicrobial-Therapy-TMO) is an innovative program for infection prevention management has been used our centre since 2019. specific features the protocol regard both prophylaxis during neutropenia (abandonment fluoroquinolone, posaconazole use high-risk patients, aerosolized liposomal amphotericin B until...
This study examined whether exposure to the Internet could create a preference for colors associated with visited Web sites and explored possible relationship self-reported problematic use deprivation.100 adult participants were divided into 2 groups; one was deprived access 4 hours, other not. After this period, they asked choose color complete series of psychometric questionnaires concerning mood (Positive Negative Affect Schedule), anxiety (Spielberger State-Trait Anxiety Inventory),...
Exosomes are extracellular vesicles playing a pivotal role in the intercellular communication. They shuttle different cargoes, including nucleic acids from their cell of origin. For this reason, they have been studied as carriers tumor markers liquid biopsy approaches, particular for solid tumors. Few data available concerning exosomes myeloid neoplasia. To better understand real potential and best approach to investigate leukemic exosomes, we present results pilot feasibility study...
Background Minimal residual disease (MRD) monitoring is an important tool to optimally address post-transplant management of acute myeloid leukemia (AML) patients. Methods We retrospectively analyzed the impact bone marrow CD34+ molecular chimerism and WT1 on outcome a consecutive series 168 AML patients submitted allogeneic stem cell transplantation. Results The cumulative incidence relapse (CIR) was significantly lower in with donor cells ≥ 97.5% < 213 copies/ABL x 10^4 both at 1 st...