Glucose-6-phosphate dehydrogenase (G6PD; EC 1.1.1.49) deficiency is a common genetic abnormality affecting an estimated 400 million people worldwide. Clinical and biochemical analyses have identified many variants exhibiting range of phenotypes, which been well characterized from the hematological point view. However, until now, their precise molecular basis has remained unknown. We cloned sequenced seven mutant G6PD alleles. In nondeficient polymorphic African variant A we found single...

Journal Article Repression of transcriptional activity at a distance by the evolutionary conserved KRAB domain present in subfamily zinc finger proteins Get access Gina Pengue, Pengue Dipartimento di Genetica, Biologia Generate e Molecolare, Universitá Feredico IIVia Mezzocannone 8, 80134 Naples, Italy Search for other works this author on: Oxford Academic PubMed Google Scholar Viola Calabró, Calabró Paola Cannada Bartoli, Bartoli Alfredo Pagliuca, Pagliuca Luigi Lania + *To whom...

Tight control of p63 protein levels must be achieved under differentiation or apoptotic conditions. Here, we describe a new regulatory pathway for the DeltaNp63alpha protein. We found that MDM2 binds in nucleus promoting its translocation to cytoplasm. The nuclear localization signal is required export and subsequent degradation, whereas ring-finger domain dispensable. Once exported cytoplasm by MDM2, targeted degradation Fbw7 E3-ubiquitin ligase. Efficient (also known as FBXW7) requires...

Genetic alteration of the p53 tumor suppressor gene, which monitors DNA damage and operates cell cycle checkpoints, is a major factor in development human malignancies. The protein belongs to family that also includes two structurally related proteins, p63 p73. Although all three proteins share similar transcriptional functions antiproliferative effects, each them appears play distinct role suppression. One principal regulators activity MDM2 protein. interaction with inhibits targets for...

P63, a protein related to the tumor suppressor p53, is transcription factor that plays an important role in epidermal differentiation and limb development. The gene has two distinct promoters allow formation of proteins either contain (TA) or lack (ΔN) transactivation domain. In addition, alternative splicing at 3' end generates with different C-termini, denoted α, β γ for total six isoforms. ΔNp63α isoform main expressed all stages development, however...

The prototype cold-shock Y-box binding protein 1 (YB-1) is a multifunctional that regulates variety of fundamental biological processes including cell proliferation and migration, DNA damage, matrix synthesis chemotaxis. plethora functions assigned to YB-1 strictly dependent on its subcellular localization. In resting cells, localizes cytoplasm where it component messenger ribonucleoprotein particles. Under stress conditions, contributes the formation granules (SGs), cytoplasmic foci...

Many pathogens involved in human infection have rapidly increased their antibiotic resistance, reducing the effectiveness of therapies recent decades. Most them can form biofilms and effective drugs are not available to treat these formations. Natural products could represent an efficient solution discovering developing new overcome antimicrobial resistance biofilm-related infections. In this study, 20 secondary metabolites produced by pathogenic fungi forest plants belonging diverse classes...

The ARF protein functions as an important sensor of hyper-proliferative stimuli restricting cell proliferation through both p53-dependent and -independent pathways. Although to date the majority studies on have focused its anti-proliferative role, few addressed whether may also pro-survival functions. Here we show for first time that during process adhesion spreading re-localizes sites active actin polymerization focal points where it interacts with phosphorylated kinase. In line recruitment...

Ten Amaryllidaceae alkaloids (AAs) were isolated for the first time from Pancratium maritimum collected in Calabria region, Italy. They belong to different subgroups of this family and identified as lycorine, which is main alkaloid, 9-O-demethyllycorine, haemanthidine, haemanthamine, 11-hydroxyvittatine, homolycorine, pancracine, obliquine, tazettine vittatine. Haemanthidine was a scalar mixture two 6-epimers, already known also other 6-hydroxycrinine alkaloids, but they separated...

The <i>INK4a</i> gene, one of the most often disrupted loci in human cancer, encodes two unrelated proteins, p16^INK4a and p14^ARF (ARF) both capable inducing cell cycle arrest. Although it has been clearly demonstrated that ARF inhibits via p53 stabilization, very little is known about involvement other regulatory pathways, as well on mechanisms responsible for activating following oncoproliferative stimuli. In search factors might associate with to control its activity...

Ectodermal dysplasias (EDs) are a group of human pathological conditions characterized by anomalies in organs derived from epithelial-mesenchymal interactions during development. Dlx3 and p63 act as part the transcriptional regulatory pathways relevant ectoderm derivatives, autosomal mutations either these genes associated with EDs. However, functional relationship between both proteins is unknown. Here,we demonstrate that downstream target p63. Moreover, we show transcription abrogated...

The epidermis is a stratified epithelium which develops depending on the transcription factor p63, member of p53 family factors. p63 strongly expressed in innermost basal layer where highly proliferative epithelial cells reside. functions as molecular switch that initiates stratification or cell fate determination while regulating proliferation and differentiation developmentally mature keratinocytes. acts upstream Dlx3 homeobox gene transcriptional regulatory pathway relevant to ectodermal...

Aloe arborescens Miller , belonging to the genus (Liliaceae family), is one of main varieties used worldwide. Although less characterized than commonest vera known be richer in beneficial phytotherapeutic, anticancer, and radio‐protective properties. It commonly as a pharmaceutical ingredient for its effect burn treatment ability increase skin wound healing However, very few studies have addressed biological effects at molecular level. The aim research provide evidences antiproliferative...

The fungal pathogens Cochliobolus australiensis and Pyricularia grisea have recently been isolated from diseased leaves of buffelgrass (Cenchrus ciliaris) in its North American range, their ability to produce phytotoxic metabolites that could potentially be used as natural herbicides against this invasive weed was investigated. Fourteen secondary obtained vitro cultures these two were tested by leaf puncture assay on the host plant at different concentrations. Radicinin (10S,...

By biochemical characterization of glucose-6-phosphate dehydrogenase (G6PD) from the red cells seventeen subjects population Matera (Southern Italy) we have identified six genetically determined common variants. Among these, G6PD Metaponto and A(-) had been already fully characterized. We now found that is heterogeneous since one two examined mutations at codons 68 126 characteristic a typical variant, while other subject only codon mutation. Pisticci Tursi are new variants whose molecular...

The tumor suppressor p14(ARF) inhibits cell growth in response to oncogenic stress a p53-dependent and independent manner. However, new physiologic roles for ARF activation have been proposed. We previously demonstrated that interacts with p63, influencing its transcriptional activity. p63 is member of the p53 family involved skin limb development, as well homeostasis mature epidermis. Here, we show that, human keratinocytes, tumor-derived lines, targets DeltaNp63alpha, most abundantly...

It is now commonly accepted that the intestinal microbiota plays a crucial role in gut physiology and homeostasis, both qualitative quantitative alterations compositions of flora exert profound effects on host's cells. In spite this, details interaction between commensal bacteria cells are still largely unknown only few cases molecular mechanisms have been elucidated. Here we analyze molecules produced secreted by Lactobacillus gasseri SF1183 human HCT116 L. well known species lactic acid...

Bacterial lipopolysaccharide (LPS) induces release of inflammatory mediators both in immune and epithelial cells. We investigated whether changes epigenetic marks, including selected histone modification DNA methylation, may drive or accompany the activation COX-2 gene HT-29 human intestinal cells upon exposure to LPS. Here we describe cyclical acetylation (H3), methylation (H3K4, H3K9, H3K27) occurring at promoter overtime after LPS stimulation. Histone K27 are carried out by H3 demethylase...

Y-box binding protein 1 (YBX-1 or YB-1) is an oncoprotein that promotes replicative immortality, tumor cell invasion and metastasis. The increase in the abundance of YB-1 translocation from cytoplasm to nucleus characteristic malignant growth. We have previously reported ΔNp63α, a transcription factor known play pivotal role keratinocyte proliferation differentiation, nuclear accumulation. Here, we show highly expressed proliferating keratinocytes down-regulated during differentiation....