A5077533509- Helicobacter pylori-related gastroenterology studies
- Cardiac electrophysiology and arrhythmias
- Gastroesophageal reflux and treatments
- Ion channel regulation and function
- Cardiac Imaging and Diagnostics
- Inflammatory mediators and NSAID effects
- Cardiac Ischemia and Reperfusion
- Advanced MRI Techniques and Applications
- Cardiovascular Function and Risk Factors
- Cardiomyopathy and Myosin Studies
- Gastrointestinal motility and disorders
- Eosinophilic Esophagitis
- Renal Transplantation Outcomes and Treatments
- Neuroscience and Neural Engineering
- Cardiac Valve Diseases and Treatments
- Neuroscience and Neuropharmacology Research
- Cardiac Arrhythmias and Treatments
- Pharmacological Effects and Toxicity Studies
- Asthma and respiratory diseases
- Nitric Oxide and Endothelin Effects
- Cardiovascular Effects of Exercise
- Mitochondrial Function and Pathology
- Pharmacological Effects of Natural Compounds
- Adipose Tissue and Metabolism
- Liver Disease Diagnosis and Treatment
Doshisha Women's College of Liberal Arts
2015-2024
Fujieda Municipal General Hospital
2021
Morinomiya Hospital
2020-2021
National Institute of Infectious Diseases
2011-2020
Hamamatsu University School of Medicine
2008-2018
Hokkaido University
1968-2018
Life Science Institute
2018
Kashiwa Municipal Hospital
2016-2017
Hamamatsu University Hospital
2014-2016
Tottori University
1999-2015
The activities of various types antiulcer agents against Helicobacter pylori (formerly called Campylobacter pylori) strains were determined by an agar dilution method. Among the compounds tested, two benzimidazole proton pump inhibitors, lansoprazole (AG-1749) and omeprazole, found to have significant this organism. activity was comparable that bismuth citrate, with MICs ranging from 3.13 12.5 micrograms/ml, fourfold more potent than omeprazole. A major metabolite acid-converted...
In heart, spontaneous local increases in cytosolic Ca2+ concentration ([Ca2+]i) called "Ca2+ sparks" may be fundamental events underlying both excitation-contraction coupling and resting leak from the sarcoplasmic reticulum (SR). this study, sparks were analyzed rabbit rat ventricular myocytes with laser scanning confocal microscopy fluorescent indicator fluo 3. During first 20 s of rest after regular electrical stimulation, frequency SR content gradually decreased rabbit. When was by...
Plasma globotriaosylsphingosine (lyso-Gb3) is a promising secondary screening biomarker for Fabry disease. Here, we examined its applicability as primary classic and late-onset disease in males females.Between 1 July 2014 31 December 2015, screened 2,359 patients (1,324 males) referred from 168 Japanese specialty clinics (cardiology, nephrology, neurology, pediatrics), based on clinical symptoms suggestive of We used the plasma lyso-Gb3 concentration, α-galactosidase A (α-Gal A) activity,...
Background —The Na + /Ca 2+ exchange (NCX) extrudes Ca from cardiac myocytes, but it can also mediate influx, load the sarcoplasmic reticulum with , and trigger release reticulum. In ischemia/reperfusion or digitalis toxicity, increased levels of intracellular [Na ] ([Na i ) may raise [Ca ([Ca via NCX, leading to cell injury arrhythmia. Methods Results —We used KB-R7943 (KBR) selectively block influx NCX study role NCX-mediated in intact rat ventricular myocytes. Removing extracellular...
The effect of Ca 2+ –calmodulin‐dependent protein kinase II (CaMKII) on excitation–contraction coupling (E–C coupling) was studied in intact ferret cardiac myocytes using the selective inhibitor KN‐93. KN‐93 decreased steady‐state (SS) twitch [Ca ] i (by 51%), resting spark frequency 88%) and SS sarcoplasmic reticulum (SR) content evaluated by caffeine application 37.5%). Increasing extracellular concentration ([Ca o ) to 5 m restored SR load towards that control (2 ), but still...
The gastric proton pump inhibitor lansoprazole, its active analog AG-2000, and omeprazole dose dependently inhibited urease activity extracted with distilled water from Helicobacter pylori cells; the 50% inhibitory concentrations were between 3.6 9.5 microM, which more potent than those of inhibitors, such as acetohydroxamic acid, hydroxyurea, thiourea. These compounds also in intact cells H. mustelae but did not inhibit ureases other bacteria, Proteus vulgaris, mirabilis, Providencia...
The antisecretory and antiulcer activities of 2[[[3-methyl-4-(2,2,2-trifluoroethoxy)-2-pyridyl]methyl] sulfinyl]-1H-benzimidazole (AG-1749) were investigated in dogs rats. AG-1749 inhibited both the (H+ + K+)-adenosine triphosphatase activity canine gastric microsomes dibutyryl cyclic AMP-stimulated acid formation isolated parietal cells suppressed secretion stimulated by histamine, pentagastrin, bethanechol or a peptone meal Heidenhain pouch dogs; ID50 values between 0.2 0.7 mg/kg p.o....
Our study aimed to investigate both the clinical implications of late gadolinium enhancement (LGE) by cardiovascular magnetic resonance (CMR) and relation LGE findings in patients with Takotsubo cardiomyopathy (TTC). We evaluated 20 consecutive (2 men, 18 women; median age, 77 years; interquartile range [IQR] 67-82 years) who were admitted our hospital diagnosis TTC. CMR was performed within 1 week after admission, follow-up studies conducted 1.5 6 months later. In 8 patients, imaging during...
FK-506 binding protein (FKBP) has been reported to be closely associated with the ryanodine receptor in skeletal and cardiac muscle modulate sarcoplasmic reticulum (SR) Ca 2+ release channel gating isolated channels. can inhibit activity of FKBP, thereby reversing its effects on SR release. We investigated function FKBP during normal contractions transients intact rat ventricular myocytes loaded fluorescent indicators. significantly increased steady state twitch contraction amplitudes even...
Reactive oxygen species (ROS) production during ischemia-reperfusion (I/R) is thought to be a critical factor for myocardial injury. However, small amount of ROS the ischemic preconditioning (IPC) may provide signal cardioprotection. We have previously reported that repetitive pretreatment [hydrogen peroxide (H(2)O(2)), 2 microM] mimicked IPC-induced cardioprotection in Langendorff-perfused rat hearts. further investigated mechanisms ROS-induced against I/R injury and tested hypothesis...
Gastroesophageal reflux disease (GERD) and psychological stress are associated with sleep disturbances. The aim of the present study was to examine prevalence disturbances, anxiety, depression by GERD subtypes identify factors disturbances in general population.A total 2002 Japanese subjects, who underwent annual health checkups, were enrolled asked fill out a questionnaire, including frequency scale for symptoms (FSSG), Athens Insomnia Scale (AIS), Rome III Hospital Anxiety Depression...
Novel 2'-deoxycytidine antimetabolites, specifically several 2'-modified 2'-deoxy-4'-thiocytidines, were synthesized as potential new antineoplastic agents. Methyl 3-O-benzylxylofuranoside was converted to a 1,4-anhydro-4-thioarabitol 24. Protection of the primary alcohol 24 gave common intermediate (15) which useful for synthesis various 2'-deoxy-4'-thionucleosides. Oxidation secondary hydroxyl group 15, followed by Wittig reaction or treatment with (diethylamido)sulfur trifluoride (DAST)...
Alterations in cellular Ca2+ transport and excitation-contraction coupling may contribute to dysfunction cardiac hypertrophy. Left ventricular myocytes were isolated from rat hearts after 15-18 wk of suprarenal abdominal aortic banding evaluate the hypothesis that hypertrophy alters relationship between current (ICa) sarcoplasmic reticulum (SR) load during steady-state voltage-clamp depolarization. Mean arterial pressure (MAP) heart weight-to-body weight ratio banded (B) animals...
The effect of an erythromycin derivative, EM-523, on gastrointestinal motility was investigated in conscious dogs and compared with that motilin cisapride, trimebutine metoclopramide. In the fasting state, EM-523 given i.v. or i.d. at 3 micrograms/kg more induced contractions stomach migrated along small intestine. pattern very similar to by motilin. digestive increased amplitude gastric contractions. Cisapride metoclopramide both states; however, their contractile different from EM-523....
Antisecretory drugs such as histamine H<sub>2</sub>-receptor antagonists (H<sub>2</sub>-RAs) and proton pump inhibitors (PPIs) are commonly used for the treatment of gastric duodenal ulcers induced by nonsteroidal anti-inflammatory (NSAIDs). However, effects these on NSAID-induced small intestinal not fully understood. The H<sub>2</sub>-RAs PPIs gastrointestinal lesions motility were examined in rats. Male Wistar rats (180–220 g) used. Indomethacin (10 mg/kg) was administered orally fasted...