Canine bacterial keratitis is a common infection that can potentially threaten vision. Staphylococcus pseudintermedius ( S. ) an opportunistic pathogen has been isolated from the canine conjunctival sac but there are only few reports on role of this bacterium in keratitis. This study focused distribution rate sac, and antibiotic resistance, biofilm-producing ability, dissemination virulence factors strains healthy dogs with The included 35 40 Bacterial species were confirmed by...

Abstract Staphylococcus aureus is a pathogen that the causative agent of several human and veterinary infections plays critical role in clinical subclinical mastitis cattle. Autophagy conserved defence mechanism eukaryotes. Studies have reported S can subvert autophagy survive cells. survival cells an important cause chronic persistent infection. However, it unclear whether escape innate immune In this study, initiation due to presence was detected bovine macrophages. We observed autophagic...

Bovine mastitis is a prevalent disease that causes serious economic problems globally in the dairy industry. Staphylococcus aureus an important pathogen of bovine mastitis. This study was conducted to characterize S. isolates from clinical cases large-scale herds China. isolated 624 and confirmed by matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI-TOF MS). In total, 62 were obtained. Cluster analysis, genetic diversity, quantification biofilm formation,...

Mitochondria are cellular organelles that involved in various metabolic processes, and damage to mitochondria can affect cell health even lead disease. Mitophagy is a mechanism by which cells selectively wrap degrade damaged maintain homeostasis. However, studies have not focused on whether mitophagy the occurrence of Staphylococcus aureus (S. aureus)-induced mastitis dairy cows. Here, we found S. infection bovine macrophages leads oxidative damage. The expression LC3, PINK1 Parkin was...

Abstract Problem Endometritis is a common disease that affects dairy cow reproduction. Autophagy plays vital role in cellular homeostasis and modulates inflammation by regulating interactions with innate immune signaling pathways. However, little known about the regulatory relationship between autophagy bovine endometrial epithelial cells (BEECs). Thus, we aimed to determine of inflammatory response BEECs. Methods Study In present study, expression levels proinflammatory cytokines were...

Avian pathogenic Escherichia coli (APEC) causes bloodstream infections mainly by resisting the bactericidal action of host serum. Although various protein and polysaccharide factors involved in serum resistance have been identified, role small non-coding RNA (sRNA) has rarely studied. The sRNA RyhB contributes to APEC, but regulation mechanism resistance-related targets remains unknown. Here, we studied regulatory on capsule synthesis how regulates resistance, macrophage phagocytosis...

Abstract The bovine uterus is susceptible to infection, and the elevated cortisol level due stress are common in cows after delivery. essential trace element selenium plays a pivotal role antioxidant anti‐inflammatory defence system of body. This study investigated whether supplementation protected endometrial cells from inflammation presence high‐level cortisol. primary epithelial were subjected Escherichia coli lipopolysaccharide establish cellular model. gene expression inflammatory...

Abstract Bovine endometritis severely inhibits uterine repair and causes considerable economic loss. Besides, parturition-induced high cortisol levels inhibit immune function, reduce cell proliferation, further tissue repair. Selenium (Se) is an essential trace element for animals to maintain normal physiological function has powerful antioxidant functions. This study investigated whether Se supplementation reduces endometrial damage promotes in cows with under stress explored the underlying...

Sodium fluoride (NaF) is associated with embryonic and fetal development abnormalities, but the mechanism by which this occurs unclear. DNA methylation, an important epigenetic reprogramming mechanism, essential for normal development. Thus, we investigated effect of NaF on methylation in early mouse embryos, as well sperm liver using bisulfite sequencing ELISA. Data indicate that H19, a paternally imprinted gene, compared to control was less methylated 8-cell embryos from pregnant mice...

African swine fever (ASF) is mainly an acute hemorrhagic disease which highly contagious and lethal to domestic pigs wild boars. The global pig industry has suffered significant economic losses due the lack of effective vaccine treatment. virus (ASFV) a large genome 170–190 kb, encoding more than 150 proteins. During infection, ASFV evades host innate immunity via multiple viral A528R very important member polygene family ASFV, was shown inhibit IFN-β production by targeting NF-κB, but its...

Aberrant expression of tripartite motif 11 (TRIM11) and the Wnt/β-catenin pathway are essential for facilitating tumorigenesis progression in multiple types cancer. To investigate molecular changes linking dysregulation TRIM11 activation gastric cancer (GC) progression. The levels were detected GC tissues cells by immunohistochemistry western blotting. role growth, proliferation, invasion was observed a series cell functional experiments further verified vivo. Co-immunoprecipitation (Co-IP),...

Endometritis represents a prevalent condition in perinatal dairy cows. Bovine endometrial epithelial cells (BEECs), as the primary interface between cavity and external environment, are particularly vulnerable to infection by pathogenic bacteria following parturition. A20 is essential for regulating inflammation modulating immune responses. Nevertheless, exact role of BEECs response inflammatory not fully understood. An endometritis model infected

Primary canine corneal epithelial cells (CCECs) easily become senescent, and cell proliferation is limited. Therefore, sampling for experimentation requires a large number of animals, which problematic in terms animal welfare fails to maintain the stability vitro analyses.In this study, CCECs were separated purified by trypsin dispase II enzymatic analysis. Next, immortalized transfection with lentiviral vector expressing Simian vacuolating virus 40 T (SV40T). The line (CCEC-SV40T) was...

Type VI secretion system (T6SS) is a secretory found in Gram-negative bacteria. One of the main structures for T6SS Hcp (hemolysin co-regulation protein) pipeline. To investigate role major sub-unit genes hcp1 and hcp2 , we deleted constructing in-frame gene deletion mutants. The properties biofilm formation adhesion to chicken embryo fibroblasts cells (DF1 cells) were reduced mutant. knockout ability avian pathogenic Escherichia coli (APEC) strain CE129 infect developing embryos. expression...

Abstract Small non-coding RNA RyhB is a key regulator of iron homeostasis in bacteria by sensing availability the environment. Although known to influence bacterial virulence interacting with metabolism related regulators, its interaction genes, especially Type III secretion system (T3SS), has not been reported. Here, we demonstrate that two paralogs Salmonella enterica serovar Enteritidis upregulate (T3SS) effectors, and consequently affect invasion into intestinal epithelial cells....

Abstract Mounting evidence has proposed the importance of Wnt/β-catenin pathway and tripartite motif 31 (TRIM31) in certain malignancies. Our research aimed to clarify correlation between aberrant TRIM31 expression during gastric cancer (GC) oncogenesis development. was drastically elevated GC tissues closely associated with aggressive clinical outcomes poor prognosis. Moreover, downregulation attenuated cell proliferation invasion vitro. Mechanistically, could bind ubiquitinate Axin1...

An elevated endogenous cortisol level due to the peripartum stress is one of risk factors postpartum bovine uterine infections. Selenium a trace element that elicits anti-inflammation and antioxidation properties. This study aimed reveal modulatory effect selenium on inflammatory response primary endometrial stromal cells in presence high-level cortisol. The were subjected lipopolysaccharide establish cellular inflammation. mRNA expression toll-like receptor 4 (TLR4), proinflammatory...

Stress and infection seriously threaten the reproductive performance health of dairy cows. Various perinatal stresses increase plasma cortisol concentrations in cows, chronically high levels may incidence severity uterine diseases. Selenium (Se) enhances antioxidant capacity The aim this study was to explore how Se affects oxidative stress primary bovine endometrial stromal cells (BESC) with background. reactive oxygen species (ROS) other biomarkers were measured using flow cytometry assay...