A5040419215- RNA modifications and cancer
- Cancer-related gene regulation
- Wnt/β-catenin signaling in development and cancer
- Cancer-related Molecular Pathways
- Hippo pathway signaling and YAP/TAZ
- Ubiquitin and proteasome pathways
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- interferon and immune responses
- Cancer Mechanisms and Therapy
- Peptidase Inhibition and Analysis
- FOXO transcription factor regulation
- Ferroptosis and cancer prognosis
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Circular RNAs in diseases
- Galectins and Cancer Biology
- Cancer, Lipids, and Metabolism
- Lung Cancer Treatments and Mutations
- Colorectal Cancer Treatments and Studies
- NF-κB Signaling Pathways
- Pancreatic and Hepatic Oncology Research
- Protease and Inhibitor Mechanisms
- Anorectal Disease Treatments and Outcomes
- Autophagy in Disease and Therapy
First Affiliated Hospital of Nanchang University
2016-2025
Nanchang University
2016-2025
Jiangxi Provincial Cancer Hospital
2022-2023
Wenzhou Medical University
2021
Tulane University
2017-2018
Pancreatic cancer is characterized by aggressive and highly metastatic phenotypes. This disease exhibits a poor patient prognosis considered challenge due to the limited treatment options encountered in clinical practice. Previous studies have shown that ruscogenin, saponin found root of Ophiopogon japonicus, exerts wide range biological functions including anticancer activity. In present study, effects ruscogenin were investigated on pancreatic cells potential molecular mechanism this...
// Jun Deng 1, * , Wan Lei 1 Xiaojun Xiang Ling Zhang Yu Gong Meijiao Song Yi Wang Ziling Fang Feng Miao Ze Sun Chen Zhengyu Zhan Jianping Xiong Department of Oncology, The First Affiliated Hospital Nanchang University, 330006, PR China first author Correspondence to: Xiong, e-mail: jpxiong@medmail.com Keywords: CUL4A, Hippo, miR-9, miR-137, gastric cancer Received: September 05, 2015 Accepted: December 07, Published: January 28, 2016 ABSTRACT Although Cullin 4A...
Abstract Background Large tumor suppressor kinase 1 (LATS1), one of the predominant components Hippo pathway, has been characterized as a key player controlling proliferation and invasion cancer cells, including gastric (GC) cells. However, mechanism by which functional stability LATS1 is modulated yet to be elucidated. Methods Online prediction tools, immunohistochemistry western blotting assays were used explore expression WW domain-containing E3 ubiquitin ligase 2 (WWP2) in GC cells...
Increasing evidence highlights the important roles of tripartite motif containing 24 (TRIM24) in tumor initiation and malignant progression many tumors, including gastric cancer (GC). Although TRIM24 expression is remarkably upregulated during GC carcinogenesis, molecular mechanisms underlying dysregulation remain unexplored.In this study, miRNA target prediction tools were applied to explore miRNAs that potentially TRIM24. Western blot quantitative reverse-transcriptase PCR (qRT-PCR)...
// Bo Cao 1 , Ziling Fang 1,2 Peng Liao Xiang Zhou 1,3 Jianping Xiong 2 Shelya Zeng and Hua Lu Department of Biochemistry & Molecular Biology, Tulane Cancer Center, University School Medicine, New Orleans, LA, USA The First Affiliated Hospital Nanchang University, Nanchang, People's Republic China 3 Fudan Shanghai Center the Institutes Biomedical Sciences, Shanghai, Correspondence to: Lu, email: Keywords : RPL22/eL22, p53, MDM2, RPL11/uL5, RPL5/uL18 Received July 25, 2017 Accepted September...
Ribosomal proteins (RPs) play important roles in modulating the MDM2-p53 pathway. However, less is known about upstream regulators of RPs. Here, we identify SPIN1 (Spindlin 1) as a novel binding partner human RPL5/uL18 that for this ablation activates p53, suppresses cell growth, reduces clonogenic ability, and induces apoptosis cancer cells. Mechanistically, sequesters uL18 nucleolus, preventing it from interacting with MDM2, thereby alleviating uL18-mediated inhibition MDM2 ubiquitin...
The Yes-associated protein (YAP1) is a main effector of the canonical Hippo pathway, which contributes greatly to tumor initiation, progression, and metastasis in multiple cancers, including gastric cancer (GC). Due limited knowledge YAP1 upregulation cancer, it great challenge therapeutic targets toward Hippo–YAP1 pathway. Here, we identify nucleolar spindle-associated 1 (NUSAP1) as novel binding partner YAP1. NUSAP1 associated with unfavorable clinical outcomes GC patients, depletion...
Tripartite motif-containing 24 (TRIM24) is important in tumor development and progression. However, the role of TRIM24 gastric cancer (GC) mechanisms underlying dysregulated expression remain to be fully elucidated. In present study, it was found that frequently overexpressed GC cell lines tissues compared with normal controls, as determined by western blotting immunohistochemical staining. The high nuclear correlated depth invasion (P=0.007), tumor-node-metastasis stage (P=0.005), lymph...
Aberrant expression of tripartite motif 11 (TRIM11) and the Wnt/β-catenin pathway are essential for facilitating tumorigenesis progression in multiple types cancer. To investigate molecular changes linking dysregulation TRIM11 activation gastric cancer (GC) progression. The levels were detected GC tissues cells by immunohistochemistry western blotting. role growth, proliferation, invasion was observed a series cell functional experiments further verified vivo. Co-immunoprecipitation (Co-IP),...
Accumulating evidence has highlighted the critical role of cullin 4B (CUL4B) in driving tumourigenesis several malignancies, including gastric cancer (GC); however, mechanisms underlying CUL4B upregulation remain unclear. The dysregulation microRNAs (miRNAs or miRs) is known to be involved tumourigenesis. In this study, we report that expression miR‑381 and miR‑489 downregulated negatively correlated with GC tissues cell lines. Further analysis verified directly targeted CUL4B. silencing...
Coiled-coil domain-containing 68 (CCDC68) plays different roles in cancer and is predicted as a tumor suppressor human colorectal (CRC). However, the specific role of CCDC68 CRC underlying mechanisms remain unknown. Here, we showed that expression was lower than corresponding normal tissues, level positively correlated with disease-free survival. Ectopic decreased cell proliferation vitro suppressed growth xenograft tumors vivo . caused G0/G1 cycle arrest, downregulated CDK4, upregulated...
Low-density lipoprotein receptor-related protein 8 (LRP8) is involved in the development of multiple tumors, including lung cancer. However, exact mechanism by which LRP8 exerts its oncogenic role non-small cell cancer (NSCLC) remains elusive. Hence, this study, we aimed to unravel expression and progression NSCLC. We used online bioinformatics databases identify types validated NSCLC lines tissues Western blotting immunohistochemistry. The functions carcinogenesis were determined using...
Our study aimed to investigate the clinical significance and biological functions of Spindlin1 (SPIN1) in colorectal cancer (CRC) tumorigenesis progression, as well mechanism underlying its upregulation. The expression SPIN1 was detected by immunohistochemistry western blotting assays. Bioinformatics prediction dual-luciferase reporter assays were used determine whether microRNA-381 (miR-381) could target SPIN1. A series cell functional experiments performed miR-381-mediated regulation is...
Mounting evidence highlights the essential role of TRIM44 in tumor initiation and malignant progression several cancers; however, function osteosarcoma (OS) remains unknown. In this study, we aim to investigate reveal its regulation by deregulated miRNAs OS.The expression profiles were examined immunohistochemistry, Western blotting, qRT-PCR. The biological functions investigated through siRNA-mediated knockdown experiments. miR-410 was confirmed dual luciferase reporter assays, rescue...
Malignant cell growth and chemoresistance, the main obstacles in treating gastrointestinal cancer (GIC), rely on Hippo p53 signalling pathways. However, upstream regulatory mechanisms of these pathways remain complex poorly understood.Immunohistochemistry (IHC), western blot RT-qPCR were used to analyse expression RNF146, miR-3133 key components pathway. CCK-8, colony formation, drug sensitivity assays murine xenograft models investigate effect RNF146 GIC. Further exploration mechanism was...
Endoscopic sclerotherapy is a widely used minimally invasive procedure for internal hemorrhoids, yet postoperative symptoms remain concern.
Abstract Mounting evidence has proposed the importance of Wnt/β-catenin pathway and tripartite motif 31 (TRIM31) in certain malignancies. Our research aimed to clarify correlation between aberrant TRIM31 expression during gastric cancer (GC) oncogenesis development. was drastically elevated GC tissues closely associated with aggressive clinical outcomes poor prognosis. Moreover, downregulation attenuated cell proliferation invasion vitro. Mechanistically, could bind ubiquitinate Axin1...
Abstract: Cullin 4A ( CUL4A ) overexpression has been reported to be involved in the carcinogenesis and progression of many malignant tumors. However, role gastric cancer (GC) remains unclear. In this study, we explored whether how regulates proinflammatory signaling promote GC cell invasion. Our results showed that knockdown inhibited migration invasion induced by lipopolysaccharide (LPS) stimulation. We also found both nuclear factor-kappa B (NF-κB) protein expressions were enhanced LPS...
Monoclonal antibodies that target the PD-1 receptor are emerging as promising therapeutic candidates for treatment of biliary tract cancers (BTCs). The purpose current study was to assess combination camrelizumab with chemotherapy a first-line metastatic BTCs.We conducted prospective single-arm pilot antibody (camrelizumab 3 mg/kg d1, Q2 W or Q3 W) combined different regimens BTCs. Efficacy endpoints were objective response rate (ORR), disease control (DCR), progression free survival (PFS),...