A5107462803- Genetics, Aging, and Longevity in Model Organisms
- Evolution and Genetic Dynamics
- Neurobiology and Insect Physiology Research
- Mitochondrial Function and Pathology
- Insect Utilization and Effects
- Evolutionary Game Theory and Cooperation
- Circadian rhythm and melatonin
- Genomics and Phylogenetic Studies
- Invertebrate Immune Response Mechanisms
- Dietary Effects on Health
- Autophagy in Disease and Therapy
- Insurance, Mortality, Demography, Risk Management
- Aging and Gerontology Research
- Mathematical Biology Tumor Growth
- Epigenetics and DNA Methylation
- Ethics in medical practice
- Insect and Arachnid Ecology and Behavior
- Genetic Neurodegenerative Diseases
- Single-cell and spatial transcriptomics
- Scientific Research and Philosophical Inquiry
- Global Health Care Issues
- CRISPR and Genetic Engineering
- Spaceflight effects on biology
- Adipose Tissue and Metabolism
- Mosquito-borne diseases and control
Institut Jacques Monod
2024-2025
Université Paris Cité
2012-2024
Sorbonne Paris Cité
2015-2024
Inserm
2020-2024
Center for Research and Interdisciplinarity
2020-2023
Laboratoire de Tribologie et Dynamique des Systèmes
2023
Evolution et ingénierie de systèmes dynamiques
2023
Unit of Functional and Adaptive Biology
2015-2019
Centre National de la Recherche Scientifique
2010-2018
Sorbonne Université
2018
Aging is characterized by a growing risk of disease and death, yet the underlying pathophysiology poorly understood. Indeed, little known about how functional decline individual organ systems relates to integrative physiology aging probability death organism. Here we show that intestinal barrier dysfunction correlated with lifespan across range Drosophila genotypes environmental conditions, including mitochondrial dietary restriction. Regardless chronological age, predicts impending in...
Alterations in the composition of intestinal microbiota have been correlated with aging and measures frailty elderly. However, relationships between microbial dynamics, age-related changes physiology, organismal health remain poorly understood. Here, we show that dysbiosis microbiota, characterized by an expansion Gammaproteobacteria, is tightly linked to age-onset barrier dysfunction Drosophila. Indeed, alterations precede predict onset aged flies. Changes occurring prior contribute...
Aberrant protein aggregation and mitochondrial dysfunction have each been linked to aging a number of age-onset neurodegenerative disorders, including Parkinson disease. Loss-of-function mutations in parkin, an E3 ubiquitin ligase that functions promote the ubiquitin-proteasome system degradation also quality control, implicated heritable forms The question whether parkin can modulate or positively impact longevity, however, has not addressed. Here, we show ubiquitous neuron-specific...
AMPK exerts pro-longevity effects in diverse species; however, the tissue-specific mechanisms involved are poorly understood.Here, we show that up-regulation of adult Drosophila nervous system induces autophagy both brain and also intestinal epithelium.Induction is linked to improved homeostasis during aging extended lifespan.Neuronal autophagy-specific protein kinase Atg1 necessary sufficient induce these inter-tissue prolong lifespan.Furthermore, intestine cell autonomously...
Abstract The accumulation of dysfunctional mitochondria has been implicated in aging, but a deeper understanding mitochondrial dynamics and mitophagy during aging is missing. Here, we show that upregulating Drp1—a Dynamin-related protein promotes fission—in midlife, prolongs Drosophila lifespan healthspan. We find short-term induction Drp1, sufficient to improve organismal health prolong lifespan, observe midlife shift toward more elongated morphology, which linked the aged flight muscle....
Abstract Aging’s most obvious characteristic is the time dependent increase of an individual’s probability to die. This lifelong process accompanied by a large number molecular and physiological changes. Although numerous genes involved in aging have been identified past decades its leading factors yet be determined. To identify very processes driving we developed years assay physiologically old individuals synchronized population Drosophila melanogaster . Those show age-dependent intestinal...
The Smurf Assay (SA) was initially developed in the model organism Drosophila melanogaster where a dramatic increase of intestinal permeability has been shown to occur during aging (Rera et al., 2011). We have since validated protocol multiple other organisms (Dambroise 2016) and utilized assay further our understanding (Tricoire Rera, 2015; Rera 2018). SA now also used by labs assess barrier (Clark Katzenberger Barekat 2016; Chakrabarti Gelino 2016). itself is simple; however, numerous...
Cardiac aging is a complex process, which influenced by both environmental and genetic factors. Deciphering the mechanisms involved in heart senescence therefore requires identifying molecular pathways that are affected age controlled conditions. We describe functional genomic investigation of control cardiac Drosophila. Molecular signatures were identified differential transcriptome analysis followed detailed bio-informatic analysis. This approach implicated JNK/dJun pathway transcription...
There is no clear-cut boundary between Free and Open Source Software Scholarship, the histories, practices, fundamental principles two remain complex. In this study, we critically appraise intersections differences movements. Based on our thematic comparison here, conclude several key things. First, there substantial scope for new communities of practice to form within scholarly that place sharing collaboration/open participation at their focus. Second, Both practices FOSS can be more deeply...
Background: Dissecting the functional impact of genetic mutations is essential to advancing our understanding genotype-phenotype relationships and identifying new therapeutic targets. Despite progress in sequencing CRISPR technologies, proteome-wide mutation effect prediction remains challenging. Here, we introduce ProteoCast, a scalable interpretable computational method for classification variants protein site identification. It relies solely on evolutionary information, leveraging...
<title>Abstract</title> <bold>Background:</bold> Dissecting the functional impact of genetic mutations is essential to advancing our understanding genotype-phenotype relationships and identifying new therapeutic targets. Despite progress in sequencing CRISPR technologies, proteome-wide mutation effect prediction remains challenging. Here, we introduce ProteoCast, a scalable interpretable computational method for classification variants protein site identification. It relies solely on...
A functional decline in tissue stem cells and mitochondrial dysfunction have each been linked to aging multiple aging-associated pathologies. However, the interplay between energy homeostasis, cells, organismal remains poorly understood. Here, we report that expression of single-subunit yeast alternative NADH dehydrogenase, ndi1, Drosophila intestinal progenitor delays onset markers extends lifespan. In addition, ndi1 intestine increases feeding behavior results weight gain. Consistent with...
Aging is commonly described as being a continuous process affecting progressively organisms time passes. This results in progressive decrease individuals fitness through wide range of both organismal–decreased motor activity, fertility, resistance to stress–and molecular phenotypes–decreased protein and energy homeostasis, impairment insulin signaling. In the past 20 years, numerous genes have been identified playing major role aging process, yet little known about events leading that loss...
The wealth of genomic data has boosted the development computational methods predicting phenotypic outcomes missense variants. most accurate ones exploit multiple sequence alignments, which can be costly to generate. Recent efforts for democratizing protein structure prediction have overcome this bottleneck by leveraging fast homology search MMseqs2. Here, we show usefulness strategy mutational outcome through a large-scale assessment 1.5M variants across 72 families. Our study demonstrates...
Signs of ageing become apparent only late in life, after organismal development is finalized. Ageing, most notably, decreases an individual’s fitness. As such, it commonly perceived as a non-adaptive force evolution and considered by-product natural selection. Building upon the evolutionarily conserved age-related Smurf phenotype, we propose simple mathematical life-history trait model which organism characterized by two core abilities: reproduction homeostasis. Through simulation this...
Ageing is characterised at the molecular level by six transcriptional 'hallmarks of ageing', that are commonly described as progressively affected time passes. By contrast, 'Smurf' assay separates high-and-constant-mortality risk individuals from healthy, zero-mortality individuals, based on increased intestinal permeability. Performing whole body total RNA sequencing, we found Smurfness distinguishes changes associated with chronological age those biological age. We show heterogeneity...
Abstract Cuticle pigmentation was shown to be associated with body temperature for several relatively large species of insects, but it questioned small insects. Here we used a thermal camera assess the association between drosophilid cuticle and increase when individuals are exposed light. We compared mutants effects within ( Drosophila melanogaster ebony yellow mutants). Then analyzed impact naturally occurring variation complexes americana/Drosophila novamexicana yakuba/Drosophila santomea...
Friedreich’s ataxia (FA) is a rare neurodegenerative disease which very debilitating for the patients who progressively lose their autonomy. The lack of efficient therapeutic treatment strongly argues urgent need to search new active compounds that may stop progression or prevent appearance symptoms when genetic defect diagnosed early enough. In present study, we used yeast strain with deletion frataxin homologue gene as model FA cells in primary screen two chemical libraries, fraction...
ABSTRACT Friedreich's ataxia (FA) is caused by reduced levels of frataxin, a highly conserved mitochondrial protein. There currently no effective treatment for this disease, which characterized progressive neurodegeneration and cardiomyopathy, the latter being most common cause death in patients. We previously developed Drosophila melanogaster cardiac model FA, fly frataxin inactivated specifically heart, leading to heart dilatation impaired systolic function. Methylene Blue (MB) was...
Human ageing, along with the ageing of conventional model organisms, is depicted as a continuous and progressive decline biological capabilities accompanied by an exponentially increasing mortality risk. However, not all organisms experience identically our understanding phenomenon coloured human-centric views. Ageing multifaceted influences diverse range species in varying ways. Some undergo swift declines post-reproduction, while others exhibit insubstantial changes throughout their...