A5059851212- Acute Myeloid Leukemia Research
- Hematopoietic Stem Cell Transplantation
- Epigenetics and DNA Methylation
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Cancer Genomics and Diagnostics
- Hematological disorders and diagnostics
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- Histone Deacetylase Inhibitors Research
- Immune Cell Function and Interaction
- RNA Research and Splicing
- Glycosylation and Glycoproteins Research
- Multiple Myeloma Research and Treatments
- MicroRNA in disease regulation
- Ubiquitin and proteasome pathways
- Receptor Mechanisms and Signaling
- Immune cells in cancer
- Cancer-related molecular mechanisms research
- Protein Degradation and Inhibitors
- Cellular transport and secretion
- Sarcoma Diagnosis and Treatment
- Virus-based gene therapy research
- Cancer therapeutics and mechanisms
- Neurogenetic and Muscular Disorders Research
- Cancer, Hypoxia, and Metabolism
University Medical Center Groningen
2010-2024
University of Groningen
2009-2022
Dialyse Centrum Groningen
2020
Memorial Sloan Kettering Cancer Center
2016-2019
The microRNA-99 (miR-99) family comprises a group of broadly conserved microRNAs that are highly expressed in hematopoietic stem cells (HSCs) and acute myeloid leukemia (LSCs) compared with their differentiated progeny. Herein, we show miR-99 regulates self-renewal both HSCs LSCs. maintains HSC long-term reconstitution activity by inhibiting differentiation cell cycle entry. Moreover, inhibition induced LSC depletion an MLL-AF9-driven mouse model AML, leading to reduction leukemia-initiating...
Despite significant efforts to improve therapies for acute myeloid leukemia (AML), clinical outcomes remain poor. Understanding the mechanisms that regulate development and maintenance of leukemic stem cells (LSCs) is important reveal new therapeutic opportunities. We have identified CD97, a member adhesion class G protein–coupled receptors (GPCRs), as frequently up-regulated antigen on AML blasts critical regulator blast function. High levels CD97 correlate with poor prognosis, silencing...
Most of our knowledge the effects aging on hematopoietic system comes from studies in animal models. In this study, to explore potential human stem and progenitor cells (HSPCs), we evaluated CD34+ derived young (<35 years) old (>60 adult bone marrow with respect phenotype vitro function. We observed an increased frequency phenotypically defined age, but no distinct differences functional capacity. Given that regeneration peripheral blood counts can serve as a readout HSPCs, compared various...
Patients who have undergone autologous stem cell transplantation are subsequently more susceptible to chemotherapy-induced bone marrow toxicity. In the present study, primitive progenitor cells were examined one year after and compared with normal mobilized peripheral blood cells. Post-transplantation contained a significantly lower percentage of quiescent in CD34+/CD38low fraction marrow. addition, we observed strong decrease cell/primitive frequency post-transplantation CD34+ as defined by...
Aging is generally considered to be the consequence of stem cell attrition caused by activity tumor suppressor pathways that censor potentially malignant clones eliciting apoptosis or senescence. An important effector aging cyclindependent kinase inhibitor p16(INK4a), which also a known cancer. The expression p16(INK4a) very low absent in young organisms but increases with advancing age. We recently showed that, unlike healthy cells, acute myeloid leukemia (AML) derived blasts show...
Mutations of nucleophosmin 1 are frequently found in acute myeloid leukemia and lead to aberrant cytoplasmic accumulation protein. Immunohistochemical staining is therefore recommended as the technique choice front-line screening. In this study, we assessed sensitivity specificity immunohistochemistry on formalin-fixed bone marrow biopsies compared with gold standard molecular analysis predict mutation status 119 patients leukemia. Discrepant cases were further characterized by gene...
Abstract Acute myeloid leukemia (AML) often presents as an oligoclonal disease whereby multiple genetically distinct subclones can coexist within patients. Differences in signaling and drug sensitivity of such complicate treatment warrant tools to identify them track progression. We previously identified &gt;50 AML-specific plasma membrane (PM) proteins, 7 these (CD82, CD97, FLT3, IL1RAP, TIM3, CD25, CD123) were implemented routine diagnostics patients with AML (n = 256) myelodysplastic...
The myelodysplastic syndromes (MDS) are primarily a disease of the elderly, but it is unclear whether aging itself an independent contributor to pathophysiology these disorders. While normal aged hematopoietic system and MDS share many features compared young hematopoiesis, they also exhibit important differences. With demonstration that mutations present in can be identified healthy elderly individuals, will elucidate differences interactions between MDS.