A5004226245- Nematode management and characterization studies
- Legume Nitrogen Fixing Symbiosis
- Plant Virus Research Studies
- Advances in Cucurbitaceae Research
- Plant-Microbe Interactions and Immunity
- Entomopathogenic Microorganisms in Pest Control
- Plant Pathogens and Fungal Diseases
- Plant Disease Resistance and Genetics
- Research in Cotton Cultivation
- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- Mycotoxins in Agriculture and Food
- Microbial Natural Products and Biosynthesis
- Studies on Chitinases and Chitosanases
- Plant Disease Management Techniques
- Plant Molecular Biology Research
- Plant biochemistry and biosynthesis
- Plant Stress Responses and Tolerance
- Plant Parasitism and Resistance
- Soybean genetics and cultivation
- Cocoa and Sweet Potato Agronomy
- Marine and fisheries research
- Insect Resistance and Genetics
- Plant tissue culture and regeneration
- CRISPR and Genetic Engineering
Institute of Vegetables and Flowers
2016-2025
Chinese Academy of Agricultural Sciences
2016-2025
Shanxi Agricultural University
2024
Public Health Clinical Center of Chengdu
2023
Ministry of Agriculture and Rural Affairs
2016-2019
Chinese Academy of Fishery Sciences
2018
Cotton Research Institute
2008-2012
Texas A&M University
2008
Abstract Background WRKY proteins are a large family of transcriptional regulators in higher plant. They involved many biological processes, such as plant development, metabolism, and responses to biotic abiotic stresses. Prior the present study, only one full-length cucumber protein had been reported. The recent publication draft genome sequence allowed us conduct genome-wide search for proteins, compare these positively identified with their homologs model plants, Arabidopsis . Results We...
Purpureocillium lilacinum of Ophiocordycipitaceae is one the most promising and commercialized agents for controlling plant parasitic nematodes, as well other insects pathogens. However, how fungus functions at molecular level remains unknown. Here, we sequenced two isolates (PLBJ-1 PLFJ-1) P. from different places Beijing Fujian. Genomic analysis showed high synteny isolates, phylogenetic indicated they were related to insect pathogen Tolypocladium inflatum. A comparison with species...
Losses in crops caused by plant pathogenic bacteria and parasitic nematode are increasing because of a decrease efficacy traditional management measures. There is an urgent need to develop nonchemical ecofriendly based control diseases. A potential approach controlling disease the use biocontrol agents their secondary metabolites (SMs). Luckily fungi especially genus Trichoderma comprise great number fungal strains that producer bioactive metabolites. In this study from ten spp. were...
Cabbage Fusarium wilt (CFW) is a devastating disease caused by the soil-borne fungus oxysporum f. sp. conglutinans (Foc). One of optimal measures for managing CFW employment tolerant/resistant cabbage varieties. However, interplay between plant genotypes and pathogen Foc in shaping rhizosphere microbial community, consequent influence these assemblages on biological resistance, remains inadequately understood.
The fungus Pochonia chlamydosporia parasitizes nematode eggs and has become one of the most promising biological control agents (BCAs) for plant-parasitic nematodes, which are major agricultural pests that cause tremendous economic losses worldwide. complete mitochondrial (mt) genome is expected to open new avenues understanding phylogenetic relationships evolution invertebrate-pathogenic fungi in Hypocreales. mitogenome sequence P. 25,615 bp size, containing 14 typical protein-coding genes,...
Root-knot nematodes (Meloidogyne spp.) are soilborne pathogens that infect vegetable crops and cause major economic losses worldwide annually. Therefore, there is an urgent need for novel nematicides or biological control agents to reduce the damage caused by root-knot nematodes. In this study, we tested efficacy of Bacillus cereus strain Bc-cm103, isolated from rhizoplane Cucumis metuliferus, against Meloidogyne incognita. Strain Bc-cm103 fermentation broth 100% mortality nematode...
ABSTRACT Cucumber production is seriously constrained by Meloidogyne incognita . Because no resistance resources to the pathogen have been reported, disabling susceptibility genes may represent a novel breeding strategy introduce against this nematode in cucumber. Here, we studied clade V MLO for their involvement interaction between cucumber and M. Our results showed that Arabidopsis mutants were resistant has three genes, CsaMLO1 , CsaMLO8 CsaMLO11 with upregulated expression upon...
Fusarium oxysporum is a soil-born fungus that induces wilt and root rot on variety of plants. F. f. sp. conglutinans (Foc) can cause disease cabbage. This study showed homolog SIX1 protein in the Arabidopsis infecting isolate Fo5176 (Fo5176-SIX1) had four isoforms conidia Foc by proteomic analysis. Thus, we analyzed roles Foc-SIX1. Gene expression analysis that, compared to mycelia, dramatically altered Foc-SIX1 could be detected after cabbages, was highly expressed under axenic culture...
Fusarium oxysporum is a soil-inhabiting fungus that induces vascular wilt and root rot in variety of plants. F. f. sp. conglutinans (Foc), which comprises two races, can cause disease cabbage. Compared with race 1 (52557(-TM), R1), 2 (58385(-TM), R2) exhibits much stronger pathogenicity. Here, we provide the first proteome reference maps for Foc mycelium conidia identify 145 proteins different abundances among races. Of these proteins, most high-abundance R2 are involved carbohydrate, amino...
Meloidogyne incognita is highly specialized parasite that interacts with host plants using a range of strategies. The effectors are synthesized in the esophageal glands and secreted into plant cells through needle-like stylet during parasitism. In this study, based on RNA-seq bioinformatics analysis, we predicted 110 putative contain nuclear localization signals (NLSs). Combining Burkholderia glumae-pEDV screening system subcellular localization, from 20 randomly selected NLS effector...
Bacillus cereus strain Bc-cm103 shows nematicidal activity and, therefore, has been used as a biological control agent to the root-knot nematode Meloidogyne incognita. However, it remains unknown whether volatile organic compounds (VOCs) produced by B. are effective in biocontrol against M. Therefore, this study, we investigated of VOCs The significantly repelled second-stage juveniles (J2s) In vitro evaluation fermentation three-compartment Petri dish revealed mortality rates incognita J2s...
Summary Cucumis metuliferus (African horned cucumber), a wild relative of sativus (cucumber) and melo (melon), displays high‐level resistance to several important plant pathogens (e.g., root‐knot nematodes viruses). Here, we report chromosome‐level genome assembly for C. , with 316 Mb sequence comprising 29 039 genes. Phylogenetic analysis related species in family Cucurbitaceae indicated that the divergence time between melon was 17.8 million years ago. Comparisons genomes revealed large...
Summary Root‐knot nematodes, Meloidogyne spp., secrete effectors to modulate plant immune responses and establish a parasitic relationship with host plants. However, the functions targets of C‐type lectin (CTL)‐like incognita remain unknown. Here, we characterized CTL‐like effector M. , MiCTL1a, identified its target role in nematode parasitism. In situ hybridization demonstrated expression MiCTL1 subventral glands; planta immunolocalization showed secretion during Virus‐induced gene...
Root-knot nematodes (RKN; Meloidogyne spp.) cause a significant decrease in the yield of cucumber crops every year. Cucumis metuliferus is an important wild germplasm that has resistance to RKN which plant root volatiles are thought play role. However, underlying molecular mechanism unclear. To investigate it, we used resistant C. line CM3 and susceptible Xintaimici (XTMC). roots repelled incognita second-stage larvae (J2s), while XTMC plants attracted larvae. were found contain similar...
The entomopathogenic fungus Beauveria pseudobassiana strain RGM 2184 can reach a maximum efficacy of 80% against the quarantine pest Lobesia botrana in field assays. In this study, genome was sequenced, and mining analyses were performed to predict factors involved its insecticidal activity. Additionally, metabolic profiling RMG culture’s supernatants analyzed by mass spectrometry, activity from one these extracts evaluated Galleria mellonella larvae. analysis resulted 114 genes encoding for...
Trichoderma hamatum is a filamentous fungus that serves as biological control agent for multiple phytopathogens and an important resource promising fungicides. However, the lack of adequate knockout technologies has hindered gene function biocontrol mechanism research this species. This study obtained genome assembly T. T21, with 41.4 Mb sequence comprising 8170 genes. Based on genomic information, we established CRISPR/Cas9 system dual sgRNAs targets screening markers. plasmid donor DNA...
Sweetness and appearance of fresh fruits are key palatable preference attributes for consumers often controlled by multiple genes. However, fine-mapping the loci or genes interest single genome-based genetic analysis is challenging. Herein, we present chromosome-level genome assembly 1 landrace melon accession (Cucumis melo ssp. agrestis) with wild morphologic features thus construct a pan-genome atlas via integrating sequenced datasets. Our comparative genomic reveals total 3.4 million...
A defence response can be induced by nonpathogenic Fusarium oxysporum CS-20 in several crops, but the molecular mechanism has not been clearly demonstrated. In present study, we analysed of a susceptible cucumber cultivar (Cucumis sativus L. 9930) against pathogen (F. f. sp. cucumerinum) through root precolonization CS-20. challenge inoculation assay indicated that disease severity index (DSI) was reduced, ranging from 18.83 to 61.67 comparison with control. Root colonization analysis did...
Plant-parasitic nematodes (PPNs) are among the most serious phytopathogens and cause widespread damage in major crops. In this study, using a genome mining method, we identified nonribosomal peptide synthetase (NRPS)-like enzymes genomes of plant-parasitic nematodes, which conserved with two consecutive reducing domains at N-terminus (A-T-R1-R2) homologous to fungal NRPS-like ATRR. We experimentally investigated roles enzyme (MiATRR) nematode (Meloidogyne incognita) parasitism. Heterologous...
Abstract Root-knot nematodes (RKN) represent extensive challenges to Cucurbitaceae crops. However, Cucumis metuliferus ( Cm ) is known be resistant Meloidogyne incognita Mi infections. Thus, analysis of differentially expressed genes may lead a comprehensive gene expression profiling the incompatible - interaction. In this study, time-course transcriptome against infection was monitored using RNA-Seq. More than 170000 transcripts were examined in roots, and 2430 subsequently identified as...
Root-knot nematodes (RKNs) are highly specialized parasites that interact with their host plants using a range of strategies. The esophageal glands the main places where synthesize effector proteins, which play central roles in successful invasion. Meloidogyne incognita MiISE5 is exclusively expressed within subventral cells and upregulated during early parasitic stages. In this study, we show can be secreted to barley through infectious hyphae Magnaporthe oryzae. Transgenic Arabidopsis...
Abstract Pochonia chlamydosporia infects eggs and females of economically important plant-parasitic nematodes. The fungal isolates parasitizing different nematodes are genetically distinct. To understand their intraspecific genetic differentiation, parasitic mechanisms, adaptive evolution, we assembled seven putative chromosomes P. strain 170 isolated from root-knot nematode (~44 Mb, including 7.19% transposable elements) compared them with the genome 123 (~41 Mb) cereal cyst nematode. We...