A5029093502- Nematode management and characterization studies
- Legume Nitrogen Fixing Symbiosis
- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Entomopathogenic Microorganisms in Pest Control
- Plant Pathogens and Fungal Diseases
- Advances in Cucurbitaceae Research
- Plant Disease Resistance and Genetics
- Plant Disease Management Techniques
- Mycorrhizal Fungi and Plant Interactions
- Genomics and Phylogenetic Studies
- Plant Molecular Biology Research
- Studies on Chitinases and Chitosanases
- Insect symbiosis and bacterial influences
- Mycotoxins in Agriculture and Food
- Insect Resistance and Genetics
- Microbial Natural Products and Biosynthesis
- Plant Parasitism and Resistance
- Soybean genetics and cultivation
- MicroRNA in disease regulation
- Parasite Biology and Host Interactions
- Insect and Arachnid Ecology and Behavior
- Peptidase Inhibition and Analysis
- Plant biochemistry and biosynthesis
- Agronomic Practices and Intercropping Systems
Institute of Vegetables and Flowers
2016-2025
Chinese Academy of Agricultural Sciences
2016-2025
Henan Institute of Science and Technology
2014
Hunan Agricultural University
2010
Abstract Background WRKY proteins are a large family of transcriptional regulators in higher plant. They involved many biological processes, such as plant development, metabolism, and responses to biotic abiotic stresses. Prior the present study, only one full-length cucumber protein had been reported. The recent publication draft genome sequence allowed us conduct genome-wide search for proteins, compare these positively identified with their homologs model plants, Arabidopsis . Results We...
Purpureocillium lilacinum of Ophiocordycipitaceae is one the most promising and commercialized agents for controlling plant parasitic nematodes, as well other insects pathogens. However, how fungus functions at molecular level remains unknown. Here, we sequenced two isolates (PLBJ-1 PLFJ-1) P. from different places Beijing Fujian. Genomic analysis showed high synteny isolates, phylogenetic indicated they were related to insect pathogen Tolypocladium inflatum. A comparison with species...
Losses in crops caused by plant pathogenic bacteria and parasitic nematode are increasing because of a decrease efficacy traditional management measures. There is an urgent need to develop nonchemical ecofriendly based control diseases. A potential approach controlling disease the use biocontrol agents their secondary metabolites (SMs). Luckily fungi especially genus Trichoderma comprise great number fungal strains that producer bioactive metabolites. In this study from ten spp. were...
Abstract Fungal bioremediation is a promising approach to remove heavy-metal from contaminated water. Present study examined the ability of an earthworm gut fungus Trichoderma brevicompactum QYCD-6 tolerate and both individual multi-metals. The minimum inhibitory concentration (MIC) heavy metals [Cu(II), Cr(VI), Cd(II) Zn(II)] against was ranged 150–200 mg L −1 on composite medium, MIC Pb(II) highest with 1600 potato dextrose (PD) medium. presented metal removal rate (97.5%) which mostly...
is obligate parasitic nematode with a wide variety of hosts that causes huge economic losses every year. In an effort to identify novel bacterial biocontrols against
Our recent research revealed that pinewood nematode (PWN) possesses few genes encoding enzymes for degrading α-pinene, which is the main compound in pine resin. In this study, we examined role of PWN microbiome xenobiotics detoxification by metagenomic and bacteria culture analyses. Functional annotation metagenomes illustrated benzoate degradation its related metabolisms may provide metabolic pathways microbiome, obviously different from uses cytochrome P450 metabolism as pathway...
Cabbage Fusarium wilt (CFW) is a devastating disease caused by the soil-borne fungus oxysporum f. sp. conglutinans (Foc). One of optimal measures for managing CFW employment tolerant/resistant cabbage varieties. However, interplay between plant genotypes and pathogen Foc in shaping rhizosphere microbial community, consequent influence these assemblages on biological resistance, remains inadequately understood.
The fungus Pochonia chlamydosporia parasitizes nematode eggs and has become one of the most promising biological control agents (BCAs) for plant-parasitic nematodes, which are major agricultural pests that cause tremendous economic losses worldwide. complete mitochondrial (mt) genome is expected to open new avenues understanding phylogenetic relationships evolution invertebrate-pathogenic fungi in Hypocreales. mitogenome sequence P. 25,615 bp size, containing 14 typical protein-coding genes,...
Summary Large amounts of effectors are secreted by the oesophageal glands plant‐parasitic nematodes, but their molecular mode action remains largely unknown. We characterized a Meloidogyne incognita protein disulphide isomerase (PDI)‐like effector (MiPDI1) that facilitates nematode parasitism. In situ hybridization showed MiPDI1 was expressed specifically in subventral M . It significantly upregulated during parasitic stages. Immunolocalization demonstrated secretion planta migration and...
Root-knot nematodes (Meloidogyne spp.) are soilborne pathogens that infect vegetable crops and cause major economic losses worldwide annually. Therefore, there is an urgent need for novel nematicides or biological control agents to reduce the damage caused by root-knot nematodes. In this study, we tested efficacy of Bacillus cereus strain Bc-cm103, isolated from rhizoplane Cucumis metuliferus, against Meloidogyne incognita. Strain Bc-cm103 fermentation broth 100% mortality nematode...
ABSTRACT Cucumber production is seriously constrained by Meloidogyne incognita . Because no resistance resources to the pathogen have been reported, disabling susceptibility genes may represent a novel breeding strategy introduce against this nematode in cucumber. Here, we studied clade V MLO for their involvement interaction between cucumber and M. Our results showed that Arabidopsis mutants were resistant has three genes, CsaMLO1 , CsaMLO8 CsaMLO11 with upregulated expression upon...
Fusarium oxysporum is a soil-inhabiting fungus that induces vascular wilt and root rot in variety of plants. F. f. sp. conglutinans (Foc), which comprises two races, can cause disease cabbage. Compared with race 1 (52557(-TM), R1), 2 (58385(-TM), R2) exhibits much stronger pathogenicity. Here, we provide the first proteome reference maps for Foc mycelium conidia identify 145 proteins different abundances among races. Of these proteins, most high-abundance R2 are involved carbohydrate, amino...
Meloidogyne incognita is highly specialized parasite that interacts with host plants using a range of strategies. The effectors are synthesized in the esophageal glands and secreted into plant cells through needle-like stylet during parasitism. In this study, based on RNA-seq bioinformatics analysis, we predicted 110 putative contain nuclear localization signals (NLSs). Combining Burkholderia glumae-pEDV screening system subcellular localization, from 20 randomly selected NLS effector...
Bacillus cereus strain Bc-cm103 shows nematicidal activity and, therefore, has been used as a biological control agent to the root-knot nematode Meloidogyne incognita. However, it remains unknown whether volatile organic compounds (VOCs) produced by B. are effective in biocontrol against M. Therefore, this study, we investigated of VOCs The significantly repelled second-stage juveniles (J2s) In vitro evaluation fermentation three-compartment Petri dish revealed mortality rates incognita J2s...
Summary Cucumis metuliferus (African horned cucumber), a wild relative of sativus (cucumber) and melo (melon), displays high‐level resistance to several important plant pathogens (e.g., root‐knot nematodes viruses). Here, we report chromosome‐level genome assembly for C. , with 316 Mb sequence comprising 29 039 genes. Phylogenetic analysis related species in family Cucurbitaceae indicated that the divergence time between melon was 17.8 million years ago. Comparisons genomes revealed large...
Summary Root‐knot nematodes, Meloidogyne spp., secrete effectors to modulate plant immune responses and establish a parasitic relationship with host plants. However, the functions targets of C‐type lectin (CTL)‐like incognita remain unknown. Here, we characterized CTL‐like effector M. , MiCTL1a, identified its target role in nematode parasitism. In situ hybridization demonstrated expression MiCTL1 subventral glands; planta immunolocalization showed secretion during Virus‐induced gene...
Root-knot nematodes (RKN; Meloidogyne spp.) cause a significant decrease in the yield of cucumber crops every year. Cucumis metuliferus is an important wild germplasm that has resistance to RKN which plant root volatiles are thought play role. However, underlying molecular mechanism unclear. To investigate it, we used resistant C. line CM3 and susceptible Xintaimici (XTMC). roots repelled incognita second-stage larvae (J2s), while XTMC plants attracted larvae. were found contain similar...
Trichoderma hamatum is a filamentous fungus that serves as biological control agent for multiple phytopathogens and an important resource promising fungicides. However, the lack of adequate knockout technologies has hindered gene function biocontrol mechanism research this species. This study obtained genome assembly T. T21, with 41.4 Mb sequence comprising 8170 genes. Based on genomic information, we established CRISPR/Cas9 system dual sgRNAs targets screening markers. plasmid donor DNA...
A defence response can be induced by nonpathogenic Fusarium oxysporum CS-20 in several crops, but the molecular mechanism has not been clearly demonstrated. In present study, we analysed of a susceptible cucumber cultivar (Cucumis sativus L. 9930) against pathogen (F. f. sp. cucumerinum) through root precolonization CS-20. challenge inoculation assay indicated that disease severity index (DSI) was reduced, ranging from 18.83 to 61.67 comparison with control. Root colonization analysis did...