is obligate parasitic nematode with a wide variety of hosts that causes huge economic losses every year. In an effort to identify novel bacterial biocontrols against

Our recent research revealed that pinewood nematode (PWN) possesses few genes encoding enzymes for degrading α-pinene, which is the main compound in pine resin. In this study, we examined role of PWN microbiome xenobiotics detoxification by metagenomic and bacteria culture analyses. Functional annotation metagenomes illustrated benzoate degradation its related metabolisms may provide metabolic pathways microbiome, obviously different from uses cytochrome P450 metabolism as pathway...

Specific endophytes with biocontrol potential might occur in diseased plant tissues. We isolated an endophytic fungus from tomato root galls infected Meloidogyne incognita and identified it as Acremonium implicatum based on morphology internal transcribed spacer sequences. Its was tested vitro pot field experiments. In the test, 96.0% of second-stage juveniles M. were killed by a culture filtrate A. after 48 h. The also suppressed egg hatching, only 36.3% treated eggs hatching compared 87.3%...

Continuous cropping in greenhouses can result root-knot nematode outbreaks resulting from imbalances the soil community. However, changes communities with continuous crop production are unclear. We compared after 2 years (2-yr) and 10 (10-yr) of by 18S rDNA high-throughput sequencing. Compared 2-yr greenhouse, 10-yr greenhouse showed acidification, nutrient accumulation salinization. Bacterial-feeding nematodes (BF) were dominant over whole growing season, but plant-parasitic (PP) group...

Soil nematodes are considered indicators of soil quality due to their immediate responses changes in the environment and plants. However, little is known about effects plant genotypes on nematode community. To elucidate this, high-throughput sequencing gas chromatography/mass spectrometry analysis was conducted analyze community structure root exudates rhizosphere tomatoes with different resistance Meloidognye incognita. The dominant group resistant Acrobeloides, while susceptible tolerant...

Diverse fungal endophytes live in plants and are shaped by some abiotic biotic stresses. Plant disease as particular stress possibly gives an impact on the communities of endophytes. In this study, clubroot caused obligate biotroph protist, Plasmodiophora brassicae, was considered to analyze its influence endophyte community using internal transcribed spacer (ITS) through high-throughput sequencing culture-dependent methods. The results showed that diversity healthy roots much higher than...

A significant population of biocontrol microorganisms resides in the rhizosphere plants, which can be utilized for plant disease control. To explore potential soil as agents against pepper blight, a bacterial strain Pa608 was screened from and identified Pseudomonas aeruginosa through morphological characteristics 16S rRNA sequences. The result showed that demonstrated antagonistic activity Phytophthora capsici , effectively suppressing mycelial growth. potted experiment high control...

Abstract Background Endophytic bacteria are considered as symbionts living within plants and influenced by abiotic biotic environments. Pathogen cause stress, which may change physiology of affect the endophytic bacterial communiy. Here, we reveal how in tumorous stem mustard ( Brassica juncea var. tumida) affected plant physiological changes caused Plasmodiophora brassicae using 16S rRNA high-throughput sequencing. Results The results showed that Proteobacteria was dominant group both...

It is well known that plant genotype can regulate phyllosphere fungi at the species level. However, little about how varieties shape fungal communities in phyllosphere. In this study, four types of maize with various levels resistances to Exserohilum turcicum were subjected high-throughput sequencing reveal properties influences composition communities. The dominant genera for all Alternaria different relative abundances, followed by Nigrospora. Hierarchical clustering analysis, non-metric...

Sclerotinia sclerotiorum is one of the most damaging and economically important necrotrophic plant pathogens, infecting more than 400 species globally. Although phenylpyrrole fungicide fludioxonil has high activity against S. sclerotiorum, reports indicate that there also substantial potential for development resistance. However, current study investigating five fludioxonil-resistant laboratory mutants found a significant fitness cost associated with resistance resulting in significantly (P...

Endophytic strains were isolated from different parts of a healthy "Dongzao" jujube (Ziziphus jujuba Mill. 'Dongzao') to find biocontrol agents against shrunken-fruit disease caused by Alternaria alternata. The screened using A. alternata strain CN193 as the target pathogen. nutrient competition for all isolates was studied dual culture, and their inhibitive capability tested measuring inhibition width filter paper disks with filtrate. Influence filtrate selected strong mycelial growth on...

Abstract Diverse fungal endophytes live in plants and are shaped by some abiotic biotic stresses. Plant disease as particular stress possibly gives an impact on the communities of endophytes. In this study, clubroot caused obligate biotroph protist, Plasmodiophora brassicae , was considered to analyze its influence endophyte community using internal transcribed spacer (ITS) through high-throughput sequencing culture-dependent methods. The results show that diversity healthy roots much higher...

The complete mitochondrial genome of the nematophagous fungus Acremonium implicatum is reported for first time. concatenated with 22,367 bp in length, encoding 13 protein-coding genes, 2 ribosomal RNA genes and a set 17 transfer genes. synteny analysis reveals that 50.35% A. sequences matched to 48.21% chrysogenum 85.68% identity. Two proteins cox3 nad6, as well seven tRNAs are lost mitogenome compared mitogenome. gene orders different, which mainly due location nad4 cox2. In addition, one...

Abstract Continuous cropping usually resulted in a series of soil problems, especially root knot nematode disaster greenhouse because imbalance community. However, the dynamic change community with various continuous years during plant growing season is unclear. Here, communities after 2 and 10 were comparatively analyzed using 18S rDNA high-throughput sequencing to reveal changing processes communities. Compared 2-year greenhouse, 10-year showed acidification, nutrients accumulation,...

Occult breast cancer (OBC) is a special type of presenting as axillary lymph node metastasis with undetectable primary lesions in the breast. Due to its low incidence and unique clinical manifestations, there lack consensus on diagnosis treatment OBC. We report case OBC treated neoadjuvant chemotherapy combined anlotinib. The was well tolerated, patient achieved pathologic complete response.

Abstract Background: Endophytic bacteria are considered as symbionts living within plants and influenced by abiotic biotic environmemts. Here, we reveal how endophytic in tumorous stem mustard ( Brassica juncea var. tumida) affected clubroot disease using 16S rRNA high-throughput sequencing. Results: The results showed that Proteobacteria was the dominant group both healthy roots clubroots, but their abundance differed. At genus level, Pseudomonas whereas Rhodanobacter roots. Hierarchical...

HomeMolecular Plant-Microbe Interactions®Vol. 35, No. 6Genome Sequence Resource of Sarocladium terricola TR, an Endophytic Fungus as a Potential Biocontrol Agent Against Meloidogyne incognita Previous RESOURCE ANNOUNCEMENT OPENOpen Access licenseGenome incognitaXueliang Tian, Tingting Sun, Runmao Lin, Rui Liu, Yuhong Yang, Bingyan Xie, and Zhenchuan MaoXueliang Tianhttps://orcid.org/0000-0001-5774-4631Henan Engineering Research Center Biological Pesticide & Fertilizer Development Synergistic...

Dwarf bunt, caused by Tilletia controversa, is a destructive disease of winter wheat (Triticum aestivum) in Ontario. The development resistant cultivars and identification effective seed treatment fungicides would be beneficial for controlling this disease. Two hundred eighty-five selected germplasm lines were evaluated T. controversa inoculated hill-plot field experiments the greenhouse from 2002 to 2005 identify sources resistance. Twenty-one genotypes, including ‘Blizzard’, ‘Carlisle’,...

XJ-25, a strain with strong antioxidant activity was isolated from sand biological soil crusts in Gurban Tonggut Desert, Xinjiang, China. Strain XJ-25 is closely related to Bacillus simplex through the 16s rDNA sequencing combined morphological, physiological and biochemical analysis. The medium for optimal NB 1.5 g/Lglucose. Based on kinetic assay, began at early exponential growth phases; maximum reached stationary phase. Scavenging effects DPPH, hydroxyl superoxide radicals,...