A5100329967- Phosphodiesterase function and regulation
- Connexins and lens biology
- Intraocular Surgery and Lenses
- Wnt/β-catenin signaling in development and cancer
- Organ Transplantation Techniques and Outcomes
- Cancer-related molecular mechanisms research
- Trace Elements in Health
- dental development and anomalies
- Ophthalmology and Visual Impairment Studies
- Mesenchymal stem cell research
- Anorectal Disease Treatments and Outcomes
- Liver Disease and Transplantation
- Cancer-related Molecular Pathways
- Ureteral procedures and complications
- MicroRNA in disease regulation
- Liver Disease Diagnosis and Treatment
- TGF-β signaling in diseases
- Neurological diseases and metabolism
- Genetic factors in colorectal cancer
- Inflammatory mediators and NSAID effects
- Periodontal Regeneration and Treatments
- Cancer-related gene regulation
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
USA Mitchell Cancer Institute
2021-2023
University of South Alabama
2021-2023
Northwest University
2023
Shanxi Eye Hospital
2023
Dalian Medical University
2019-2021
Chongqing Medical University
2019-2021
Second Affiliated Hospital of Chongqing Medical University
2019-2021
Shanghai Stomatological Hospital
2019
First Affiliated Hospital of GuangXi Medical University
2018
Guangxi Medical University
2018
MicroRNAs (miRNAs) play a key role in regulating cell differentiation. In the present study, we aimed to explore of miR-140-5p odontoblastic differentiation dental pulp stem cells (DPSCs). DPSCs from normal human impacted third molars were isolated and cultured. After overexpression or silencing DPSCs, activity, proliferation, evaluated. The possible target gene was verified by luciferase reporter assay. Using transfection technology, RT-CPR, Western blot confirm regulates through...
Oxidative stress drives protein S-glutathionylation, which regulates the structure and function of target proteins is implicated in pathogenesis many diseases. Glutaredoxin 1 (Grx1), a cytoplasmic deglutathionylating enzyme, maintains reducing environment within cell under various conditions by reversing S-glutathionylation. Grx1 performs wide range antioxidant activities lens prevents protein-thiol mixed disulfide accumulation, protein-protein aggregation, insolubilization, apoptosis...
Colorectal cancer (CRC) is the second leading cause of cancer-related mortality in United States. CRC initiated by mutations tumor suppressor gene, adenomatous polyposis coli (APC), or β-catenin gene. These stabilize and constitutively activate Wnt/β-catenin target genes, such as c-Myc cyclin D1, ultimately to cancer. Naturally occurring stilbene derivatives, resveratrol pterostilbene, inhibit Wnt signaling repress cell proliferation but are ineffective at concentrations less than 10 μM. To...
Abstract Previous studies have reported that phosphodiesterase 10A (PDE10) is overexpressed in colon epithelium during early stages of tumorigenesis and essential for cancer cell growth. Here we describe a novel non-COX inhibitory derivative the anti-inflammatory drug, sulindac, with selective PDE10 activity, ADT 061. 061 potently inhibited growth cells expressing high levels PDE10, but not normal colonocytes do express PDE10. The concentration range by which was identical to concentrations...
Though many mutations have been identified to be associated with the occurrence of congenital cataract, pathogenic loci in some affected families are still unknown. Clinical data and genomic DNA were collected from a four-generation Chinese family. Candidate independently verified for cosegregation whole pedigree. Linkage analysis showed that disease-causing mutation was located between 1p36.21 1p36.33. Analysis whole-exome sequencing combined linkage novel variant (g.2451906C>T) at intron 4...
To study the effect of senescence marker protein 30 (SMP30) on proliferation and apoptosis human lens epithelial cell (HLEC) SRA01/04.SMP30 overexpression (OE) knock down (KD) type lines were cultivated by using two groups regucalcin (RGN; SMP30) lentiviral vectors (LV-RGN, LV-RGN-RNAi) respective negative control virus infect SRA01/04 cells. Western blot real-time quantitative polymerase chain reaction (q-PCR) analysis used to determine RGN efficiency. We use counting kit-8 (CCK8) assay...
Abstract Background: Inactivation of the TGF-β signaling occurs in 50-75% all colorectal cancer cases. Down-regulation Smad4, central mediator signaling, >50% stage III patients. How Smad4 contributes to normal intestinal homeostasis and functions as a tumor suppressor are incompletely understood. We reported that β-catenin mRNA expression is inhibited by BMP-mediated loss results biologically significant amplification Wnt/β-catenin signal. Here, we report on consequences for...
<p>NMR of compound 5.</p>
<p>Mass spectroscopy of compound 5.</p>
<p>Mass spectroscopy of compound 5.</p>
<p>Mass spectroscopy of ADT 061</p>
<p>HPLC chromatogram of ADT 061</p>
To investigate the effects of Panax notoginseng saponins (PNS) on functional status Kupffer cells (KCs) and immune environment after liver transplantation explore possible mechanisms.KCs were isolated from rats assessed for phagocytic activity viability using ink Trypan blue staining. The exposed to lipopolysaccharide (LPS) alone or in combination with PNS treatment at 0, 10 20 μmol/L. expressions inflammatory factors oxidative stress products supernatant assayed Western blotting ELISA;...