A5008199762- X-ray Diffraction in Crystallography
- Chemical Synthesis and Analysis
- Crystallization and Solubility Studies
- Click Chemistry and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Biochemical and Structural Characterization
- Monoclonal and Polyclonal Antibodies Research
- Mitochondrial Function and Pathology
- Supramolecular Self-Assembly in Materials
- ATP Synthase and ATPases Research
- Immunotherapy and Immune Responses
- Biopolymer Synthesis and Applications
- Pharmacological Effects of Natural Compounds
- Amino Acid Enzymes and Metabolism
- Immune Cell Function and Interaction
- Carbohydrate Chemistry and Synthesis
- RNA and protein synthesis mechanisms
- vaccines and immunoinformatics approaches
- Phytochemistry and biological activities of Ficus species
- Radical Photochemical Reactions
- Protein Degradation and Inhibitors
- Laser Applications in Dentistry and Medicine
- Computational Drug Discovery Methods
- Phytochemistry and Biological Activities
- Amyloidosis: Diagnosis, Treatment, Outcomes
Université de Bordeaux
2013-2025
Technologies pour la Santé
2025
Institut Européen de Chimie et Biologie
2013-2023
Centre National de la Recherche Scientifique
2013-2023
Institut Polytechnique de Bordeaux
2013-2023
Chimie et Biologie des Membranes et des Nanoobjects
2022
Institut de Biochimie et Génétique Cellulaires
2020-2022
Science for Life Laboratory
2016-2019
Karolinska Institutet
2016-2019
Karolinska University Hospital
2017-2019
Abstract In the search of molecules that could recognize sizeable areas protein surfaces, a series ten helical aromatic oligoamide foldamers was synthesized on solid phase. The comprise three to five monomers carrying various proteinogenic side chains, and exist as racemic mixtures interconverting right‐handed left‐handed helices. Functionalization by nanomolar ligand human carbonic anhydrase II (HCA) ensured they would be held in close proximity surface. Foldamer–protein interactions were...
Abstract Structural analysis of a co‐crystal helically‐folded peptide‐foldamer hybrid in complex with hDM2 E3 ubiquitin ligase, revealed unique orientation for the C ‐terminal proline pyrrolidine ring pointing backwards sequence, and suggested new opportunities macrocyclization. In particular, we found that prolyl residue could be replaced by its (2 S ,4 )‐4‐mercaptoprolyl analogue optimal bisthioether crosslinking cysteine installed at position 4 sequence. The resulting i , +7 stapled is...
Abstract Efficient optimization of a peptide lead into drug candidate frequently needs further transformation to augment properties such as bioavailability. Among the different options, foldamers, which are sequence‐based oligomers with precise folded conformation, have emerged promising technology. We introduce oligourea foldamers reduce character inhibitors protein–protein interactions (PPI). However, design mimics is currently limited by lack structural information on how these adapt...
UL18 is a human CMV (HCMV) MHC class I (MHCI) homolog that efficiently inhibits leukocyte immunoglobulin-like receptor subfamily B member 1 (LILRB1)+ NK cells. We found an association of LILRB1 polymorphisms in the regulatory regions and ligand-binding domains with control HCMV transplant patients. Naturally occurring variants expressed model cells showed functional differences classical MHCI, but not HLA-G. The altered recognition was recapitulated binding assays LILRB1. Each 4...
Abstract In the search of molecules that could recognize sizeable areas protein surfaces, a series ten helical aromatic oligoamide foldamers was synthesized on solid phase. The comprise three to five monomers carrying various proteinogenic side chains, and exist as racemic mixtures interconverting right‐handed left‐handed helices. Functionalization by nanomolar ligand human carbonic anhydrase II (HCA) ensured they would be held in close proximity surface. Foldamer–protein interactions were...
The molecular bases of amyloid aggregation propensity are still poorly understood, especially for proteins that display a stable folded native structure. A prototypic example is human beta-2 microglobulin (β2m), which, when accumulated in patients, gives rise to dialysis-related amyloidosis. Interestingly, although the physiologic concentration β2m mice five times higher than found no deposits observed mice. Moreover, murine (mβ2m) not only displays lower both vivo and vitro but also...
Abstract MHC class I downregulation represents a significant challenge for successful T cell–based immunotherapy. cell epitopes associated with impaired peptide processing (TEIPP) constitute novel category of immunogenic Ags that are selectively presented on transporter Ag processing–deficient cells. The TEIPP neoepitopes CD8 targets, derived from nonmutated self-proteins might be exploited to prevent immune escape. In this study, the crystal structure H-2Db in complex first identified...
Helically folded aromatic foldamers may constitute suitable candidates for the ab initio design of ligands protein surfaces. As preliminary steps toward exploration this hypothesis, a tethering approach was developed to detect interactions between and foldamer by confining former at surface latter. Cysteine mutants two therapeutically relevant enzymes, CypA IL4, were produced. Two series ten synthesized bearing different proteinogenic side chains either long or short linker functionalized...
Amphipathic water-soluble helices formed from synthetic peptides or foldamers are promising building blocks for the creation of self-assembled architectures with non-natural shapes and functions. While rationally designed artificial quaternary structures such as helix bundles have been shown to contain preformed cavities suitable guest binding, there no examples adaptive binding molecules by assemblies in aqueous conditions. We previously reported a foldamer 6-helix bundle that contains an...
Post-translational modifications significantly broaden the epitope repertoire for major histocompatibility class I complexes (MHC-I) and may allow viruses to escape immune recognition. Lymphocytic choriomeningitis virus (LCMV) infection of H-2b mice generates CD8+ CTL responses directed towards several MHC-I-restricted epitopes including peptides GP92 (CSANNSHHYI) GP392 (WLVTNGSYL), both with a N-glycosylation site. Interestingly, glycosylation has different effects on immunogenicity...
Abstract Efficient optimization of a peptide lead into drug candidate frequently needs further transformation to augment properties such as bioavailability. Among the different options, foldamers, which are sequence‐based oligomers with precise folded conformation, have emerged promising technology. We introduce oligourea foldamers reduce character inhibitors protein–protein interactions (PPI). However, design mimics is currently limited by lack structural information on how these adapt...
Here we report a series of crystal structures (and accompanying biophysical data) an array diverse detergent guests bound to oligourea foldamer helix bundle. These results significantly increase our structural and chemical understanding aqueous guest recognition by foldamers will aid the design further functionalised oligourea-based self-assemblies.
Abstract Mitochondrial ATP-synthesis is catalyzed by a F1Fo-ATP synthase, an enzyme of dual genetic origin enriched at the edge cristae where it plays key role in their structure/stability. The enzyme’s biogenesis remains poorly understood, both from mechanistic and compartmentalization point view. present study provides novel molecular insights into this process through investigations on human protein called TMEM70 with unclear assembly ATP synthase. A recent has revealed existence physical...