A5057872004- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- Immunotherapy and Immune Responses
- RNA modifications and cancer
- Immune Cell Function and Interaction
- RNA Research and Splicing
- vaccines and immunoinformatics approaches
- Carbohydrate Chemistry and Synthesis
- Mosquito-borne diseases and control
- Enzyme Structure and Function
- Advanced Computational Techniques and Applications
- Advanced Sensor and Control Systems
- Nicotinic Acetylcholine Receptors Study
- Hippo pathway signaling and YAP/TAZ
- NF-κB Signaling Pathways
- Glycosylation and Glycoproteins Research
- Pneumonia and Respiratory Infections
- Plant Molecular Biology Research
- Ion channel regulation and function
- Neural dynamics and brain function
- Neuroscience and Neuropharmacology Research
- MicroRNA in disease regulation
- Metabolomics and Mass Spectrometry Studies
- Sarcoma Diagnosis and Treatment
- Malaria Research and Control
Zhejiang University
2006-2025
Karolinska University Hospital
2018-2024
Science for Life Laboratory
2018-2024
Karolinska Institutet
2018-2024
Fuzhou University
2023
Hangzhou First People's Hospital
2021-2022
Qingdao University
2022
Wuhan University of Science and Technology
2005-2022
Wuhan University
2022
Wuhan Asia Heart Hospital
2022
The non-coding RNA 7SK is the scaffold for a small nuclear ribonucleoprotein (7SKsnRNP) which regulates function of positive transcription elongation factor P-TEFb in control polymerase II metazoans. La-related protein LARP7 component 7SKsnRNP required stability and RNA. To address we determined crystal structure its La module, binds stretch uridines at 3'-end 7SK. shows that penultimate uridine tethered by two domains, La-motif RNA-recognition motif (RRM1), reveals RRM1 significantly...
MHC-I epitope presentation to CD8+ T cells is directly dependent on peptide loading and selection during antigen processing. However, the exact molecular bases underlying binding by remain largely unknown. Within peptide-loading complex, editor tapasin key of MHC-I-bound peptides. Here, we have determined an ensemble crystal structures in complex with exchange-associated dipeptide GL, as well tapasin-associated scoop loop, alone or combination candidate epitopes. These results combined...
G3BP is the central node within stress-induced protein–RNA interaction networks known as stress granules (SGs). The SG-associated proteins Caprin-1 and USP10 bind mutually exclusively to NTF2 domain of G3BP1, promoting inhibiting SG formation, respectively. Herein, we present crystal structure G3BP1-NTF2 in complex with a Caprin-1-derived short linear motif (SLiM). interacts His-31 His-62 third NTF2-binding site outside those covered by USP10, confirmed using biochemical biophysical-binding...
This study predicted the effect of strawberry anthocyanins on lipid metabolism through network pharmacology. The UPR mt mediated by ubl-5 plays a crucial role in beneficial effects Pg3G monomer nematodes under oleic acid exposure.
The compromise between affinity and specificity in TCR-dependent targeting of HLA-restricted tumor-associated antigens presents a significant challenge developing efficacious immunotherapies. As such, T cell engagers which circumvent these limitations are particular interest. We have established process to generate bispecific Designed Ankyrin Repeat Proteins (DARPins) that simultaneously target HLA-I molecules complex with peptides CD3ϵ. High-affinity HLA-A*0201/NY-ESO1 157-165 -specific...
Stress granules (SGs) are cytoplasmic condensates of proteins and RNAs that form in response to cellular stress, playing a critical role protecting cells during adverse conditions. G3BP act as central node molecular switch SG assembly. Growing evidence indicates conformational changes G3BP1 mediate its phase separation competence. However, the mechanistic basis underlying how dynamics contribute formation has remained elusive. Here, we demonstrate homodimer adopts both distinct expanded...
A new deep neural network based on the WaveNet architecture (WNN) is presented, which designed to grasp specific patterns in NMR spectra. When trained at a fixed non-uniform sampling (NUS) schedule, WNN benefits from pattern recognition of corresponding point spread function (PSF) produced by each spectral peak resulting highest quality and robust reconstruction NUS spectra as demonstrated simulations exemplified this work 2D 1H-15N correlation three representative globular proteins with...
Wound healing is a complicated event that develops in three overlapping phases: inflammatory, proliferative, and remodeling. MicroRNAs (miRNAs) have been proved to play an important role the process of skin trauma, alteration specific miRNA expression during different phases may be associated with abnormal wound healing. In this study, we determined variation miR-23b after trauma normal mice cultured cells exposed lipopolysaccharide. We further demonstrated excessive could significantly...
Viral escape from CD8+ cytotoxic T lymphocyte responses correlates with disease progression and represents a significant challenge for vaccination. Here, we demonstrate that cell recognition of the naturally occurring MHC-I-restricted LCMV-associated immune variant Y4F is restored following vaccination proline-altered peptide ligand (APL). The APL increases MHC/peptide (pMHC) complex stability, rigidifies facilitates receptor (TCR) through reduced entropy costs. Structural analyses pMHC...
BCG-activated macrophages (BAM) could kill the tumor cells through cell-cell contact. In this process membrane proteins play an important role. However, up to date, few were revealed. study, we selected a surface molecule named Trim59, which was specifically expressed on BAM (compared with negative control). We cloned and prokaryoticly extracellular domain of purified recombinant protein generated polyclonal antibodies. Immunohistochemistry showed that Trim59 abundantly in spleen, stomach...
The molecular bases of amyloid aggregation propensity are still poorly understood, especially for proteins that display a stable folded native structure. A prototypic example is human beta-2 microglobulin (β2m), which, when accumulated in patients, gives rise to dialysis-related amyloidosis. Interestingly, although the physiologic concentration β2m mice five times higher than found no deposits observed mice. Moreover, murine (mβ2m) not only displays lower both vivo and vitro but also...
Abstract The dengue protease NS2B/NS3pro has been reported to adopt either an ‘open’ or a ‘closed’ conformation. We have developed conformational filter that combines NMR with MD simulations identify ensembles dominate in solution. Experimental values derived from relaxation parameters for the backbone and methyl side chains were compared corresponding back-calculated of different obtained free simulations. Our results demonstrate high prevalence ensemble while conformation is absent,...
Pyruvate kinase (PK) deficiency is a rare genetic disorder that affects this critical enzyme within the glycolysis pathway. In recent years, Mitapivat (MTPV, AG-348) has emerged as notable allosteric activator for treating PK deficiency. However, regulatory effects exerted on by MTPV are yet to be comprehensively elucidated. To shed light molecular mechanisms of effects, we employed crystallography and biophysical methods. Our efforts yielded high-resolution crystal structure tetramer...
Abstract The serotype II Dengue (DENV 2) virus is the most prevalent of all four known serotypes. Herein, we present nearly complete 1 H, 15 N, and 13 C backbone isoleucine, valine, leucine methyl resonance assignment apo S135A catalytically inactive variant DENV 2 protease enzyme folded as a tandem formed between serine domain NS3pro cofactor NS2B, well secondary structure prediction this complex based on assigned chemical shifts using TALOS-N software. Our results provide solid ground for...
Trichinellosis is an important food-borne parasitic zoonosis throughout the world. At present, mechanisms of Trichinella spiralis infection remain unclear. Acquiring detailed information on host-Trichinella interaction would be beneficial for development new strategies trichinellosis control. Circulating miRNAs are stably detectable in blood humans and animals infected with parasites. might regulate expression target genes pathological responses during novel potential biomarkers diseases. In...
Abstract Streptococcus pneumoniae (the pneumococcus) colonizes the human nasopharynx by forming multicellular biofilms. Due to high level of asymptomatic carriage, transition infections, such as otitis media, pneumonia, sepsis, and meningitis, occurs often enough that pneumococcus remains a major cause disease death globally. Virus infection virus-induced responses, increased temperature (fever), trigger release virulent bacteria from colonizing The exact mechanisms involved in pneumococcal...
To explore the mechanisms by which circRNA/miRNA/mRNA competitive endogenous RNAs (ceRNA) networks regulate CRSwNP.The expression profiles of circRNAs, miRNAs, and mRNAs from patients with CRSwNP control subjects were acquired Gene Expression Omnibus database. The ceRNA network was constructed based on predicted circRNA-miRNA interactions miRNA-mRNA interactions. Hub-mRNAs screened protein-protein interaction analysis Cytoscape molecular complex detection. factors in tissue hsa_circ_0031594...