A5067874702- Retinal Development and Disorders
- Retinal Diseases and Treatments
- melanin and skin pigmentation
- Circadian rhythm and melatonin
- Ion-surface interactions and analysis
- Glaucoma and retinal disorders
- Electron and X-Ray Spectroscopy Techniques
- Integrated Circuits and Semiconductor Failure Analysis
- Neuroinflammation and Neurodegeneration Mechanisms
- Adenosine and Purinergic Signaling
- Retinal and Macular Surgery
- Nanoparticles: synthesis and applications
- Retinal Imaging and Analysis
- Advanced Electron Microscopy Techniques and Applications
- Metabolism and Genetic Disorders
- Retinal and Optic Conditions
- Chemical Synthesis and Analysis
- RNA and protein synthesis mechanisms
- Mitochondrial Function and Pathology
- Metabolomics and Mass Spectrometry Studies
- Neurological Disease Mechanisms and Treatments
- Parkinson's Disease Mechanisms and Treatments
- Advanced Materials Characterization Techniques
- Cancer, Hypoxia, and Metabolism
- Microbial Natural Products and Biosynthesis
Luxembourg Institute of Science and Technology
2021-2024
Max Planck Institute of Molecular Physiology
2024
STZ eyetrial
2013-2022
University of Tübingen
2011-2019
This work presents a combined light and electron microscopical approach to investigate the initial breakdown of retinal pigment epithelium (RPE) choriocapillaris (CC) in age-related macular degeneration (AMD). Perimacular sections 12 dry wet AMD eyes (82 ± 15 years) 7 age-matched controls (75 10 without pathology were investigated. Disease progression was classified into 5 stages concurrent CC breakdown. Special emphasis laid on transitions where intact CC–RPE–retina complexes went over...
Since there is evidence that the Fc domain of antivascular endothelial growth factor drugs may cause unexpected consequences in retinal and choroidal vessels, effects intravitreal ranibizumab aflibercept on monkey eyes were investigated.Four cynomolgus monkeys intravitreally injected with 0.5 mg another four 2 aflibercept. Two untreated served as controls. Funduscopy, fluorescein angiography (FA), spectral-domain-optical coherence tomography (SD-OCT) measurement intraocular pressure (IOP)...
Fundus autofluorescence is a non-invasive imaging technique in ophthalmology. Conventionally, short-wavelength (SW-AF) used for detection of lipofuscin, byproduct the visual cycle which accumulates with age or disease retinal pigment epithelium (RPE). Furthermore, near-infrared (NIR-AF) as marker RPE and choroidal melanin, but contribution lipofuscin to NIR-AF signal unclear.We employed fluorescence microscopy investigate properties melanosomes, melanolipofuscin granules histologic sections...
Stargardt disease (SD) is characterized by the accumulation of age-pigment lipofuscin in retinal pigment epithelium (RPE) and subsequent neuroretinal degeneration. The leads to vision loss early life. Here, we investigate age-dependent ultrastructural changes three SD mouse models: albino Abca4-/- pigmented Abca4-/-.Rdh8-/- mice. Since found indications for oxidative stress primarily mice, tested RPE melanin its antioxidative capabilities.SD eyes were investigated light, fluorescence...
The whitening and opacifying agent titanium dioxide (TiO2) is used worldwide in various foodstuffs, toothpastes pharmaceutical tablets. Its use as a food additive (E171 EU) has raised concerns for human health. Although the buccal mucosa first area exposed, oral transmucosal passage of TiO2 particles not been documented. Here we analyzed E171 particle translocation vivo through pig vitro on TR146 cells, effects proliferating differentiated cells. In floor pigs, isolated small aggregates were...
Over the last few decades, nanoparticles have become a key element in number of scientific and technological fields, spanning from materials science to life sciences. The characterization or samples containing nanoparticles, terms morphology, chemical composition, other parameters, typically involves investigations with various analytical tools, requiring complex workflows extending duration such studies several days even weeks. Here, we report on development new unique situ correlative...
Abstract Age‐related macular degeneration (AMD) is the leading cause of blindness in older people developed world while Stargardt's disease (SD) a juvenile and an orphan disease. Both diseases are untreatable marked by accumulation lipofuscin advancing to progressive deterioration retinal pigment epithelium (RPE) retina subsequent vision loss till blindness. We discovered that small molecule belonging tetrahydropyridoether class compounds, soraprazan renamed remofuscin, able remove existing...
Stargardt disease (STGD1), known as inherited retinal dystrophy, is caused by ABCA4 mutations. The pigmented Abca4-/- mouse strain only reflects the early stage of STGD1 since it devoid degeneration. This blue light-illuminated model presented pigment epithelium (RPE) and photoreceptor degeneration which was similar to advanced phenotype. In contrast, wild-type mice showed no RPE after light illumination. mice, acute diminished mean autofluorescence (AF) intensity in both fundus...
Accumulation of lipofuscin in the retinal pigment epithelium (RPE) is a hallmark aging and associated with degeneration encountered age-related macular (AMD) Stargardt disease (SD). Currently, treatment for lipofuscin-induced unavailable. Here, we report that Remofuscin (INN: soraprazan, tetrahydropyridoether small molecule) reverses accumulation aged primary human RPE cells non-cytotoxic SD mouse vitro. In addition, show removal after single intravitreal injection results rescue from model...
Journal Article Getting Structural and Compositional Insights into Biological Beam Sensitive Samples Using Three Complementary Detection Modalities on a Cryo-FIB Instrument Get access Olivier De Castro, Castro Advanced Instrumentation for Nano-Analytics, Materials Research Technology Department, Luxembourg Institute of Science Technology, Belvaux, Corresponding author: olivier.decastro@list.lu Search other works by this author on: Oxford Academic Google Scholar Tatjana Taubitz, Taubitz A...