A5056023717- Cell Adhesion Molecules Research
- Cystic Fibrosis Research Advances
- Endoplasmic Reticulum Stress and Disease
- Neonatal Respiratory Health Research
- Immune Response and Inflammation
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Genetics, Aging, and Longevity in Model Organisms
- Cellular transport and secretion
- Planarian Biology and Electrostimulation
- Platelet Disorders and Treatments
- Pediatric health and respiratory diseases
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- Renal and related cancers
- Adipose Tissue and Metabolism
- Immune cells in cancer
- Drug Transport and Resistance Mechanisms
- Trace Elements in Health
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Neuroscience and Neural Engineering
- Pancreatic function and diabetes
- Heat shock proteins research
- Cancer-related gene regulation
- Retinoids in leukemia and cellular processes
Kumamoto University
2016-2025
Sojo University
2013
Institute of Molecular and Cell Biology
2009-2010
Toll-like receptors (TLRs) are important sensors of microbial pathogens and mediators innate immune responses. Although the signal transduction TLRs is well elucidated, their basal regulation largely unexplored. Here we show that tumor suppressor p53 positively regulates transcription TLR3, a receptor for viral double-stranded RNA poly(I-C), by binding to site in TLR3 promoter. expression was lower HCT116 p53(-/-) cells than p53(+/+) cells. Activation 5-fluorouracil increased mRNA epithelial...
Amyloid fibril formation is associated with protein misfolding disorders, including neurodegenerative diseases such as Alzheimer's, Parkinson's, and Huntington's diseases. Familial amyloid polyneuropathy (FAP) a hereditary disease caused by point mutation of the human plasma protein, transthyretin (TTR), which binds transports thyroxine (T4). TTR variants contribute to pathogenesis amyloidosis forming fibrils in extracellular environment. A recent report showed that epigallocatechin...
Cystic fibrosis (CF) is the most common lethal inherited disorder caused by mutation in gene encoding CF transmembrane regulator (CFTR). The clinical course of characterized recurrent pulmonary infections and chronic inflammation. Here, we show that toll-like receptor-2 (TLR2) expression response were strongly enhanced human bronchial epithelial cell line, CFBE41o-. Treatment cells with 5-azacytidine decreased promoter methylation within TLR2 proximal increased endogenous non-CF 16HBE14o-...
Low-intensity electrical current (or mild stimulation; MES) influences signal transduction and activates phosphatidylinositol-3 kinase (PI3K)/Akt pathway. Because insulin resistance is characterized by a marked reduction in insulin-stimulated PI3K-mediated activation of Akt, we asked whether MES could increase Akt phosphorylation ameliorate resistance. In addition, it was also previously reported that heat shock protein 72 (Hsp72) alleviates hyperglycemia. Thus, applied combination with (HS)...
Toll-like receptor 3 (TLR3) has gained recognition as a novel molecular target for cancer therapy because TLR3 activation by its synthetic ligand poly I:C directly causes tumor cell death. Recently, we reported that suppressor p53 increases the expression of in several lines. Another study also showed interferon-alpha (IFN-alpha) up-regulates expression. We thus hypothesized various anticancer drugs such p53-activating reagents and IFNs may potentiate I:C-induced death through up-regulation...
Melinjo (Gnetum gnemon L.) seeds are rich in resveratrol (RES) and its dimers, gnetin C gnemonoside A. They have various pharmacological benefits, antioxidant anti-inflammatory properties; however, their pharmacokinetics, especially animal models, must be better understood. This study aimed to elucidate the pharmacokinetics of melinjo seed extract (MSE) components rats. Plasma samples were collected up 24 h after intergastric administration MSE (1000 mg/kg), C, or A (51 μmol/kg each). RES...
Kurozu, a traditional Japanese black vinegar (JBV), is produced from steamed unpolished rice, koji, and water through saccharification, alcoholic fermentation, acetic fermentation. These processes result in rich amino acids, vitamins, organic proteins. While Kurozu has demonstrated benefits, including anti-oxidative anti-adipogenic activities, its effects on health at the organismal level remain poorly understood. This study aimed to evaluate impact of healthspan Caenorhabditis elegans (C....
The most common cystic fibrosis transmembrane conductance regulator (CFTR) mutant in patients, Delta F508 CFTR, is retained the endoplasmic reticulum (ER) and consequently degraded by ubiquitin-proteasome pathway known as ER-associated degradation (ERAD). Because prolonged interaction of CFTR with calnexin, an ER chaperone, results ERAD calnexin seems to lead it pathway. However, role controversial. In this study, we found that overexpression partially attenuated CFTR. We observed formation...
Induction of heat shock protein (HSP) 72 improves metabolic profiles in diabetic model mice. However, its effect on pancreatic β-cells is not known. The current study investigated whether HSP72 induction can reduce β-cell stress signaling and apoptosis preserve mass. MIN6 cells db/db mice were sham-treated or treated with (HS) mild electrical stimulation (MES) (HS+MES) to induce HSP72. Several cellular markers, parameters, mass evaluated. HS+MES treatment overexpression increased levels...
The clinical course of cystic fibrosis (CF) is characterized by recurrent pulmonary infections and chronic inflammation. We have recently shown that decreased methylation the toll-like receptor-2 (TLR2) promoter leads to an apparent CF-related up-regulation TLR2. This could be responsible, in part, for CF-associated enhanced proinflammatory responses various bacterial products epithelial cells. However, molecular mechanisms underlying DNA hypomethylation-dependent enhancement TLR2 expression...
Single immunoglobulin interleukin-1 receptor-related molecule (SIGIRR) is one of the immunoglobulin-like membrane proteins that crucial for negative regulation toll-like receptor 4 (TLR4) and receptor. Despite importance understanding its expression function, knowledge limited on regulatory mechanism in epithelial tissues, such as liver, lung, gut, where predominant originally described. Here, we found SIGIRR non-epithelial innate immune cells, including primary peripheral blood monocytes,...
Metformin is widely used for the treatment of type 2 diabetes, and increasing numbers studies have shown that metformin also ameliorates tumor progression, inflammatory disease, fibrosis. However, ability to improve non-diabetic glomerular disease chronic kidney (CKD) has not been explored. To investigate effect on we a mouse model Alport syndrome (Col4a5 G5X) which were treated with or losartan, as control treatment. We investigated adriamycin-induced glomerulosclerosis model. Pathological...
Tumor suppressor genes regulate the antiviral host defense through molecular mechanisms that are not yet well explored. Here, we show tumor retinoblastoma (Rb) protein positively regulates Toll-like receptor 3 (TLR3) expression, sensing for viral double-stranded RNA and poly(I · C). TLR3 expression was lower in Rb knockout (Rb(-/-)) mouse embryonic fibroblasts (MEF) mammalian epithelial cells transfected with small-interfering (siRNA) than control cells. Consequently, induction of cytokines...