Giovanna Sabarese
A5077424551- Cancer Immunotherapy and Biomarkers
- Diabetes Treatment and Management
- Pancreatic and Hepatic Oncology Research
- Neutropenia and Cancer Infections
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Metabolism, Diabetes, and Cancer
- Cancer Genomics and Diagnostics
- Colorectal Cancer Treatments and Studies
- Inflammatory Biomarkers in Disease Prognosis
- Economic and Financial Impacts of Cancer
- Immune cells in cancer
- Advanced Breast Cancer Therapies
- Gastric Cancer Management and Outcomes
- Ferroptosis and cancer prognosis
- Medication Adherence and Compliance
- Diabetes and associated disorders
- Chronic Lymphocytic Leukemia Research
- Renal Transplantation Outcomes and Treatments
- Lung Cancer Diagnosis and Treatment
- HER2/EGFR in Cancer Research
- Esophageal Cancer Research and Treatment
- RNA modifications and cancer
- Molecular Biology Techniques and Applications
- Radiomics and Machine Learning in Medical Imaging
- Lung Cancer Treatments and Mutations
Campus Bio Medico University Hospital
2022-2024
Università Campus Bio-Medico
2021-2023
Istituto Pasteur
2018
Sapienza University of Rome
2018
Casa Sollievo della Sofferenza
2016
Istituti di Ricovero e Cura a Carattere Scientifico
2016
Novel antibody-drug conjugates against HER2 are showing high activity in HER2-negative breast cancer (BC) with low expression (i.e., 1+ or 2+ and lack of ERBB2 amplification). However, the clinical molecular features HER2-low BC yet to be elucidated. Here, we collected retrospective clinicopathological PAM50 data from 3,689 patients disease made following observations. First, proportion was higher HR-positive (65.4%) than triple-negative (TNBC, 36.6%). Second, within disease, luminal-related...
Abstract Purpose: No evidence exists as to whether type 2 diabetes mellitus (T2DM) impairs clinical outcome from immune checkpoint inhibitors (ICI) in patients with solid tumors. Experimental Design: In a large cohort of ICI recipients treated at 21 institutions June 2014 2020, we studied on glucose-lowering medications (GLM) for T2DM had shorter overall survival (OS) and progression-free (PFS). We used targeted transcriptomics subset explore differences the tumor microenvironment (TME) or...
The expression of the long noncoding RNA HOTAIR (HOX Transcript Antisense Intergenic RNA) is largely deregulated in epithelial cancers and positively correlates with poor prognosis progression hepatocellular carcinoma gastrointestinal cancers. Furthermore, functional studies revealed a pivotal role for epithelial-to-mesenchymal transition, as this causal repressive activity master factor SNAIL on genes. Despite proven oncogenic HOTAIR, its transcriptional regulation still poorly understood....
Background: MUTYH germline monoallelic variants have been detected in a number of patients affected by breast/ovarian cancer or endometrial cancer, suggesting potential susceptibility role, though their significance remains elusive since the disease mechanism is normally recessive. Hence, aim this research was to explore hypothesis that second hit could arisen other allele tumor tissue. Methods: we used Sanger sequencing and immunohistochemistry search for variant tumoral DNA assess protein...
In advanced or metastatic settings, Comprehensive Genomic Profiling (CGP) allows the evaluation of thousands gene alterations with goal offering new opportunities for personalized treatment in solid tumors. This study evaluated CGP Success Rate a real-life cohort 184 patients enrolled prospective clinical trial. data were compared routine molecular testing strategy adopted in-house. Sample age, tumor area, and percentage nuclei recorded analysis. We found that 150/184 (81.5%) samples...
Despite the advantages offered by personalized treatments, there is presently no way to predict response chemoradiotherapy in patients with non-small cell lung cancer (NSCLC). In this exploratory study, we investigated application of deep learning techniques histological tissue slides (deep pathomics), aim predicting therapy stage III NSCLC. We evaluated 35 digitalized (biopsies or surgical specimens) obtained from IIIA IIIB Patients were classified as responders (12/35, 34.7%)...
Biomarker testing is mandatory for the clinical management of patients with advanced non-small cell lung cancer (NSCLC). Myriads technical platforms are now available biomarker analysis differences in terms multiplexing capability, analytical sensitivity, and turnaround time (TAT). We evaluated performance diagnostic workflows 24 representative Italian institutions performing molecular tests on a series artificial reference specimens built to mimic routine samples. Sample sets eight slides...
<p>Tumour micron-environment transcriptome analysis.</p>
Primary acinic cell carcinoma (ACC) of the lung is an extremely rare neoplasm that more often arises near to a right bronchus. It characterized by two populations clear and dark eosinophilic cells, arranged in glandular acinar pattern. Mitosis are tumor cells show small eccentric nuclei. Positive stain for PAS, PAS-D, cytokeratin, A1AT A1ACT reported, while TTF1, p40, synaptophysin, SMA, S100 substantially negative. DOG-1 positive was observed ACC salivary glands its negativity proposed...
Trabectedin, approved for the treatment of soft tissue sarcoma (STS), interferes with cell division and genetic transcription processes. Due to its strong anti-tumor activity in only certain histotypes, several studies on trabectedin combinations are currently ongoing improve efficacy. In this study, we aimed investigate novel potential therapeutic strategies enhance effect using integrated silico, vitro, vivo approaches. For silico analysis, screened two public datasets, GSEA M5190 TCGA...
Recent years have witnessed the rise of pathomics as a mean to describe histopathological images with quantitative biomarkers for predictive and prognostic ends, combining digital pathology, omic science artificial intelligence. This novel research branch is counterpart radiomics which pursues same aims extracting knowledge from radiological images. In this paper, we present design pathomic deep learning-based system predict treatment outcome in non-small cell lung cancer patients. We...
RNA-based next-generation sequencing (RNA-seq) represents the gold standard for detecting gene fusion in non-small cell lung cancer (NSCLC). Despite this, RNA instability makes management of tissue samples extremely complex, resulting a significant number test failures with missing data or need to switch other techniques. In present study, we analyzed pre-analytical variables 140 tumor from patients affected by NSCLC detect features that increase chances successful RNA-seq. We found success...
<p>Kaplan-Meier survival estimates according to the receipt of no diabetes medication, other medications/insulin therapy only, and metformin (either alone or in combinations). A) Overall Survival whole cohort; patients not receiving medications: 18.9 months (95%CI: 15.9-21.6; 684 events), on only: 19.3 11.6-22.9; 48 metformin: 12.3 9.8-15.9; 100 events). B) Progression Free 8.2 7.1-9.4; 872 10.7 6.7-11.6; 61 7.9 5.1-10.1; 124 events).</p>
<p>Kaplan-Meier survival estimates according to the receipt of other diabetes medications and insulin therapy. A) Overall Survival whole cohort; patients on oral antidiabetic drugs therapy: 17.5 months (95%CI: 12.8-20.9; 82 events), not receiving therapy 17.8 15.4 – 19.7; 750 events). B) Progression Free 8.2 6.2-11.4; 106 8.1 7.1 9.2; 951 events).</p>
<p>Kaplan-Meier survival estimates according to the receipt of metformin. A) Overall Survival whole cohort; patients on metformin: 12.4 months (95%CI: 10.5-16.3; 100 events), not receiving 19.0 16.4 – 21.1; 732 events). B) Progression Free 7.9 5.3-10.1; 124 8.3 7.3 9.5; 933 events).</p>
<p>Volcano plot of differentially regulated genes identified by Nanostring analysis. The Benjamini–Hockberg P-values are correlated to fold-changes in transcripts diabetic samples (n = 11) versus non-diabetic controls 11). achieving the highest statistical significance (p value <0.05) highlighted presence corresponding gene name. Significantly downregulated transcripts: HRAS, Ras oncogene family (p=0.009); GTF3C1, transcription factor TFIIIC complex (p=0.018); LAG3, key immune...
<p>A) Heat map of the 770 transcripts analyzed with Nanostring Pancancer Immune Panel in diabetic samples (n=11) compared non-diabetic controls (n=11). B) selected differently transcripted genes.</p>