A5017179546- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Dermatology and Skin Diseases
- Immunodeficiency and Autoimmune Disorders
- vaccines and immunoinformatics approaches
- Immunotherapy and Immune Responses
- Urticaria and Related Conditions
- Molecular Biology Techniques and Applications
- Criminal Law and Evidence
- Adenosine and Purinergic Signaling
- Allergic Rhinitis and Sensitization
- Genomics and Phylogenetic Studies
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Criminal Justice and Corrections Analysis
- Forensic and Genetic Research
- Renal Transplantation Outcomes and Treatments
- Genomic variations and chromosomal abnormalities
- Asthma and respiratory diseases
- Chromosomal and Genetic Variations
Children's Hospital of Philadelphia
2018-2024
The Ohio State University
2018
Aristotle University of Thessaloniki
2010
Introduction: Components of the immune response have previously been associated with pathophysiology atopic dermatitis (AD), specifically Human Leukocyte Antigen (HLA) Class II region via genome-wide association studies, however exact elements not identified. Methods: This study examines genetic variation HLA genes using next generation sequencing (NGS) and evaluates resultant amino acids, particular attention on binding site residues, for associations AD. The Genetics AD cohort was used to...
HLA allelic variation has been well studied and documented in many parts of the world. However, African populations have relatively under‐represented studies variation. We characterized from 489 individuals belonging to 13 ethnically diverse rural communities countries Botswana, Cameroon, Ethiopia, Tanzania, known practice traditional subsistence lifestyles using next generation sequencing (Illumina) long‐reads Oxford Nanopore Technologies. identified 342 distinct alleles among 11 targeted...
We have developed a protocol regarding the genomic characterization of MICA gene by next generation sequencing (NGS). The amplicon includes full length and is about 13 kb. A total 156 samples were included in study. Ninety-seven these previously characterized at legacy methods (Sanger or sequence specific oligonucleotide) used to evaluate accuracy, precision, specificity, sensitivity assay. An additional 59 DNA unknown ethnicity volunteers from United States only genotyped NGS. Samples...
A number of methods have been devised to address the need for targeted genomic resequencing. One these methods, region-specific extraction (RSE) is characterized by capture long DNA fragments (15-20 kb) magnetic beads, after enzymatic extension oligonucleotides hybridized selected regions. Facilitating selection most appropriate oligos targeting a region interest, satisfying properties temperature (Tm) and entropy (ΔG), while minimizing formation primer-dimers in pooled experiment, therefore...
The human Major Histocompatibility Complex (MHC) is an approximately 4 Mb genomic segment on Chromosome 6 that plays a pivotal role in the immune response. Despite its importance various traits and diseases, complex nature makes it challenging to accurately characterize routine basis. We present novel approach allowing targeted sequencing de novo haplotypic assembly of MHC region heterozygous samples, using long-read technologies. Our validated two reference family trios, African-American...
Abstract Summary A number of methods have been devised to address the need for targeted genomic resequencing. One these methods, Region-specific extraction (RSE) DNA is characterized by capture long fragments (15-20 kb) magnetic beads, after enzymatic extension oligonucleotides hybridized selected regions. Facilitating selection most optimal oligos targeting a region interest, satisfying properties temperature (Tm) and entropy (ΔG), while minimizing formation primer dimers in pooled...