A5011630635- Mercury impact and mitigation studies
- Toxic Organic Pollutants Impact
- Heavy metals in environment
- Environmental Toxicology and Ecotoxicology
- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Pesticide Residue Analysis and Safety
- Viral Infections and Vectors
- Mycotoxins in Agriculture and Food
- Heavy Metal Exposure and Toxicity
- Asymmetric Hydrogenation and Catalysis
- Cyclopropane Reaction Mechanisms
- Identification and Quantification in Food
- Mollusks and Parasites Studies
- Dental Radiography and Imaging
- Analytical chemistry methods development
- Chromium effects and bioremediation
- Catalytic Cross-Coupling Reactions
- Herpesvirus Infections and Treatments
- Virology and Viral Diseases
- Effects and risks of endocrine disrupting chemicals
- Listeria monocytogenes in Food Safety
- Surface Chemistry and Catalysis
- Fish Biology and Ecology Studies
- Marine animal studies overview
Istituto Zooprofilattico Sperimentale della Sardegna
2014-2024
University of Sassari
1986-2022
European Commission
2017
Tokyo University of Science
1987
The emergence of new Anaplasma strains in the Mediterranean region poses a challenge for both veterinary medicine and public health, as it can lead to more complex diagnostic treatment strategies. Species related platys A. phagocytophilum, two important tick-borne pathogens, have been reported several countries. However, data on their presence Sardinia remain limited. This study aimed identify theAnaplasmaspecies genetically zoonotic agents, such phagocytophilum or platys, Sardinian horses,...
During 2009-2010, 161 tissue samples (142 placentas, 16 brains, and 3 livers) from aborted ovine fetuses on Sardinia Island, Italy, were tested for toxoplasmosis. Organs that showed a positive result by nested polymerase chain reaction (PCR) targeting the ITS1 region Toxoplasma gondii also amplified with 11 genetic markers (SAG1, 5'-SAG2, 3'-SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico) then subjected to PCR/RFLP typing. T. DNA was found in 5 14 2 livers PCR analysis all isolates...
An interlaboratory study was conducted to evaluate the effectiveness of an immunoaffinity column cleanup liquid chromatography (LC) method for determination aflatoxin B1 levels in corn samples, enforced by European Union legislation. A test portion extracted with methanol-water (80 + 20); extract filtered, diluted phosphate-buffered saline solution, filtered on a microfiber glass filter, and applied column. The washed deionized water remove interfering compounds, purified eluted methanol....
Mycoplasma agalactiae, the causative agent of contagious agalactia in small ruminants, produces a protein, named P80, that is detectable all wild-type isolates examined to date and appears expressed during early phase infection. We describe here identification, cloning expression gene encoding P80 (ma-mp81). The deduced amino acid sequence consistent with hydrophobic basic protein possesses lipoprotein signal peptide. Sequence analysis ma-mp81 suggests membrane shows significant homology...
With this study, 28 pools of snails the genus Helix, respectively Helix aspersa (n=24) and vermiculata (n=4) were analysed. They taken from snail farming stores. The Sardinia, other regions Italy, abroad. All samples examined as pool looking for these microbiological target: Salmonella spp., Listeria monocytogenes, Escherichia coli O157, Clostridium perfringens, Norovirus Hepatitis A Virus (HAV). In same pools, concentration cadmium lead by inductively coupled plasma mass spectrometry was...
Piroplasmoses are tick-borne diseases caused by hemoprotozoan parasites of veterinary and public health significance. This study focuses on the molecular identification characterization species belonging to Theileria/Babesia genera in 152 blood samples, collected from 80 horses 72 cattle several farms Sardinia, targeting 18S rRNA gene. The PCR results highlighted that 72% samples were positive for spp., with a rate infection 68% 75% cattle, respectively. Sequencing BLASTn analysis showed...
A method validation study for the determination of ochratoxin in black and white pepper (Piper spp.), nutmeg (Myristica fragrans), spice mix (blend ginger, turmeric, pepper, nutmeg, chili), cocoa powder, drinking chocolate was conducted according to International Harmonized Protocol Union Pure Applied Chemistry. The is based on extraction samples with aqueous methanol, followed by a cleanup extract an immunoaffinity column. carried out reversed-phase LC coupled fluorescence detector....
Summary A controlled carboplatin delivery system using biodegradable polymer has been used in this study. The purpose was to evaluate the local and systemic effects of injectable, microspheres containing when injected as aqueous suspension subcutaneously rats. Biocompatibility toxicity empty loaded with were evaluated by clinical histological examination. diffusion tissues time drug release platinum determination plasma over time. results study suggest that provide a sustained slow multiple...
The mechanisms of mercury accumulation and distribution in fish tissues are related to its high affinity for sulfhydryl groups proteins. There is evidence that distributed unevenly based on the different reactivity these various muscle Tuna also shows numerous specialized anatomical features including structure swimming muscles some form endothermy, which generates variations content between dark white with lipid content. aim study verify, through a suitable sub lot Thunnus thynnus caught by...
Ticks are hematophagous ectoparasites that recognized for their ability to vector a wide variety of pathogens viral, bacterial, protozoal, and helminthic nature vertebrate hosts. Among the different diseases transmitted by ticks, also called "Tick-Borne Diseases" (TBD), many zoonotic. Pathogens genus Anaplasma refer obligate intracellular bacteria within Rickettsiales order mainly through tick bites considered as well-established threats domestic animals, livestock, humans, worldwide. In...
The economic impact and losses caused by abortion of small ruminants represent an important threat to livestock industry worldwide. Infectious agents are the most commonly causes ruminant many which pose a serious human health. management outbreaks is essential understand transmission, prevention control zoonotic diseases. This study aimed increase knowledge about common known pathogens causing (Coxiella burnetii, Chlamydia abortus Toxoplasma gondii) circulating in Sardinia. In addition,...