A5101701628- Probiotics and Fermented Foods
- Protein Hydrolysis and Bioactive Peptides
- Phytochemistry and biological activities of Ficus species
- Plant Physiology and Cultivation Studies
- Plant Gene Expression Analysis
- Genomics and Phylogenetic Studies
- Horticultural and Viticultural Research
- Gut microbiota and health
- Banana Cultivation and Research
- Microbial Metabolic Engineering and Bioproduction
- Peptidase Inhibition and Analysis
- Fermentation and Sensory Analysis
- Microbial Community Ecology and Physiology
- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Proteins in Food Systems
- Food Quality and Safety Studies
- Postharvest Quality and Shelf Life Management
- Biopolymer Synthesis and Applications
- Diabetes Treatment and Management
- Meat and Animal Product Quality
- Cancer Research and Treatments
- Plant biochemistry and biosynthesis
- Plant Stress Responses and Tolerance
- Biochemical and Structural Characterization
China Agricultural University
2015-2024
Ministry of Agriculture and Rural Affairs
2022
Chengdu University
2022
Yunnan Agricultural University
2020
Sun Yat-sen University
2008-2019
Beijing Municipal Government
2010-2017
Ministry of Education of the People's Republic of China
2009-2011
Key Laboratory of Guangdong Province
2008
It has been speculated that before vertebrates evolved somatic diversity-based adaptive immunity, the germline-encoded diversity of innate immunity may have more developed. Amphioxus occupies basal position chordate phylum and hence is an important reference to evolution vertebrate immunity. Here we report first comprehensive genomic survey immune gene repertoire amphioxus Branchiostoma floridae . reported purple sea urchin a vastly expanded receptor not previously seen in other species,...
Objective Patients with renal failure suffer from symptoms caused by uraemic toxins, possibly of gut microbial origin, as deduced studies in animals. The aim the study is to characterise relationships between intestinal microbiome composition, toxins and human end-stage disease (ESRD). Design Characterisation microbiome, serum faecal metabolome phenotypes a cohort 223 patients ESRD 69 healthy controls. Multidimensional data integration reveal links these datasets use chronic kidney (CKD)...
Combined metabolomic and transcriptomic analyses were carried out with fig cultivar Green Peel its color mutant 'Purple Peel'. Five twenty-two metabolites identified as having significantly different contents between fruit peels of the two cultivars at young mature stages, respectively. Cyanidin O-malonylhexoside demonstrated a 3992-fold increase in purple peel, first identification major cyanidin fruit; 3-O-glucoside, O-hexoside cyanidin-3,5-O-diglucoside upregulated 100-fold, revealing...
Abstract Vertebrates diverged from other chordates ~500 Myr ago and experienced successful innovations adaptations, but the genomic basis underlying vertebrate origins are not fully understood. Here we suggest, through comparison with multiple lancelet (amphioxus) genomes, that ancient vertebrates high rates of protein evolution, genome rearrangement domain shuffling these greatly slowed down after divergence jawed jawless vertebrates. Compared lancelets, modern retain, at least relatively,...
Abstract Background The complex and dynamic changes during grape berry development have been studied in Vitis vinifera , but little is known about these processes other species. variety 'Norton', with a major portion of its genome derived from aestivalis maintains high levels malic acid phenolic acids the ripening berries comparison V. varieties such as Cabernet Sauvignon. Furthermore, Norton develop remarkably level resistance to most fungal pathogens while Sauvignon remain susceptible...
New dipeptidyl peptidase IV (DPP-IV)-inhibitory peptides from trypsin/chymotrypsin-treated goat milk casein hydrolysates were isolated and identified by two-dimensional silica thin-layer chromatography (2D-TLC) combined to nano LC–MS/MS. 2D-TLC with chloroform/methanol/25% ammonia (2:2:1) n-butanol/acetic acid/water (4:1:1) as the first- second-dimension eluents, respectively, in analytical semipreparative scales, was set up verified reversed-phase high-performance liquid (RP–HPLC) be...
Whole-genome shotgun assembly has been a long-standing issue for highly polymorphic genomes, and the advent of next-generation sequencing technologies made more challenging than ever. Here we present an automated pipeline, HaploMerger, reconstructing allelic relationships in diploid assembly. HaploMerger combines LASTZ-ChainNet alignment approach with novel graph-based structure, which helps to untangle between two haplotypes guides subsequent creation reference haploid assemblies. The...
Scope The present study aims to assess the antidiabetic effect of Lactobacillus paracasei strain NL41 and its potential mechanisms in rats with type 2 diabetes mellitus (T2DM) induced by a high‐fat diet low‐dose streptozotocin administration (HFD/STZ). Methods results Eighteen Sprague–Dawley (SD) are randomly assigned three groups: one control, HFD/STZ model, HFD/STZ‐ protection group for 12 weeks. Blood is collected biochemical parameters analysis tissue samples histological analysis....
The dipeptidyl peptidase IV (DPP-IV)-inhibitory bioactivity of silver carp protein (SCP) hydrolysates were investigated, and their containing efficacious DPP-IV-inhibitory peptides explored by in silico hydrolysis analysis, peptide separation combined with liquid chromatography–tandem mass spectrometry (LC–MS/MS) identification, chemical synthesis. SCP generated six proteases all showed efficient activities, Neutrase-generated had the greatest DPP-IV inhibition (IC50 1.12 mg/mL). In Neutrase...
Intestinal bifidobacteria benefit human health by promoting and modulating the gut flora, boosting therapeutic efficiency for chronic metabolic diseases cancer. Recently, Bifidobacterium adolescentis strains with high adhesion to intestinal epithelial cells were associated induction of T-helper 17 (Th17) in humans rodents. Here, two B. similar adhesive ability but different aggregation properties investigated specific immunoregulatory effects, including underlying cellular pathway, on...
The main aim of the present work was to obtain microencapsulated whey protein concentrate hydrolysate (WPCH) in order reduce its bitter taste and resistance hygroscopicity without impairing immunoregulatory activity by spray drying or freeze with (WPC) sodium alginate (SA) as carriers. To attenuate taste, WPCH were encapsulated WPC mixture (WPC/SA). splenocyte proliferation activity, hygroscopicity, morphology non-encapsulated evaluated. Results revealed that could significantly enhance...
Increasing amounts of genes have been shown to utilize alternative polyadenylation (APA) 3′-processing sites depending on the cell and tissue type and/or physiological pathological conditions at time processing, construction genome-wide database regarding APA is urgently needed for better understanding poly(A) site selection APA-directed gene expression regulation a given biology. Here we present web-accessible database, named APASdb (http://mosas.sysu.edu.cn/utr), which can visualize...
This study aimed to investigate the effects of probiotic Lactobacillus paracasei NL41 on inflammation and gut microbiota type 2 diabetic (T2D) rats induced by high-fat diet (HFD) low-dose streptozotocin (STZ). A T2D rat model was established inducing Sprague-Dawley with HFD/STZ, followed 12-weeks L. gavage. The blood, colonic tissues, feces samples these were collected for inflammation, histology, intestinal profiling. treatment remarkable improvement in inflammatory status decreasing levels...
The basic helix–loop–helix (bHLH) transcription factor family is the second largest in plants, and participates various plant growth development processes. A total of 118 bHLH genes were identified from fig ( Ficus carica L.) by whole-genome database search. Phylogenetic analysis with Arabidopsis homologs divided them into 25 subfamilies. Most s each subfamily shared a similar gene structure conserved motifs. Seventy-two found expressed at fragments per kilobase million mapped (FPKM)...
Two PPG1000 based thermo-sensitive magnetic ionic liquids were synthesized and characterized. A novel analytical method of liquid aqueous biphasic system coupled with HPLC was employed for the successive enrichment highly sensitive detection trace amount quinolones in milk first time. It has realized enrichments an High factors achieved detections sensitive. The advantages rapid pretreatment, completely free organic solvent, magnet assisted phase separation recyclability simultaneously Under...
Yak milk casein derived from Qula, a traditional Tibetan acid curd cheese, was hydrolyzed by six commercially available proteases (Trypsin, Pepsin, Alcalase, Flavourzyme, Papain and Neutrase). These hydrolysates were assayed for their inhibitory activity of Angiotensin-I-converting enzyme (ACE). The obtained Neutrase Bacillus amyloliquefaciens showed the highest ACE activity. IC 50 value Neutrase-hydrolysate 0·38 mg/ml. hydrolysate further separated consecutive ultra-filtration with 10 kDa...
Bifidobacterium longum subsp. BBMN68, an anaerobic probiotic isolated from healthy centenarian faeces, shows low oxygen (3 %, v/v) tolerance. To understand the effects of oxidative stress and mechanisms protecting against it in this strain, a proteomic approach was taken to analyse changes cellular protein profiles BBMN68 under following oxygen-stress conditions. Mid-exponential phase cells grown MRS broth at 37 °C were exposed 3 % O(2) for 1 h (I) or 9 (II), stationary subjected (III)....