A5103039357- Probiotics and Fermented Foods
- Gut microbiota and health
- Peptidase Inhibition and Analysis
- Diabetes Treatment and Management
- Globalization, Economics, and Policies
- Biochemical and Structural Characterization
- Antimicrobial Peptides and Activities
- Immune cells in cancer
- Bacterial biofilms and quorum sensing
- Protein Hydrolysis and Bioactive Peptides
- Microbial Metabolites in Food Biotechnology
- Genomics and Phylogenetic Studies
- Reproductive tract infections research
- Diet and metabolism studies
- Pancreatic function and diabetes
- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
- Bacterial Genetics and Biotechnology
- Mesenchymal stem cell research
- Traditional Chinese Medicine Studies
- Antibiotic Resistance in Bacteria
- Lipid metabolism and disorders
- Erythrocyte Function and Pathophysiology
- Bacteriophages and microbial interactions
- Neuropeptides and Animal Physiology
Guiyang Medical University
2018-2024
China Pharmaceutical University
2024
State Key Laboratory of Natural Medicine
2024
Chengdu University of Traditional Chinese Medicine
2023
China Agricultural University
2015-2023
Southwest University
2009-2023
Ministry of Agriculture and Rural Affairs
2019-2021
Karolinska Institutet
2017-2019
Karolinska University Hospital
2017-2019
Beijing Municipal Government
2015-2017
Scope The present study aims to assess the antidiabetic effect of Lactobacillus paracasei strain NL41 and its potential mechanisms in rats with type 2 diabetes mellitus (T2DM) induced by a high‐fat diet low‐dose streptozotocin administration (HFD/STZ). Methods results Eighteen Sprague–Dawley (SD) are randomly assigned three groups: one control, HFD/STZ model, HFD/STZ‐ protection group for 12 weeks. Blood is collected biochemical parameters analysis tissue samples histological analysis....
The dipeptidyl peptidase IV (DPP-IV)-inhibitory bioactivity of silver carp protein (SCP) hydrolysates were investigated, and their containing efficacious DPP-IV-inhibitory peptides explored by in silico hydrolysis analysis, peptide separation combined with liquid chromatography–tandem mass spectrometry (LC–MS/MS) identification, chemical synthesis. SCP generated six proteases all showed efficient activities, Neutrase-generated had the greatest DPP-IV inhibition (IC50 1.12 mg/mL). In Neutrase...
The anti-diabetic effects of Bifidobacterium animalis 01 and its potential mechanisms were investigated. Male SD rats given a high-fat chow diet with low-dose streptozotocin (30 mg/kg BW) to induce T2DM. supplements B. in pre- post-treatment method. Oral administration decreased food water intake, blood glucose concentration, glycosylated hemoglobin level. Treatment improved OGTT, HOMA-IR, lipid profiles, representative cytokines, liver injury markers the serum. also exhibited...
This study aimed to investigate the effects of probiotic Lactobacillus paracasei NL41 on inflammation and gut microbiota type 2 diabetic (T2D) rats induced by high-fat diet (HFD) low-dose streptozotocin (STZ). A T2D rat model was established inducing Sprague-Dawley with HFD/STZ, followed 12-weeks L. gavage. The blood, colonic tissues, feces samples these were collected for inflammation, histology, intestinal profiling. treatment remarkable improvement in inflammatory status decreasing levels...
Identification of potential therapeutic effects puerarin for the prevention hepatic insulin resistance and steatosis <italic>in vivo</italic> vitro</italic>.
Proteinaceous bioactive substances and pharmaceuticals are most conveniently administered orally. However, the facing problems side effects of proteolytic degradation denaturation in gastrointestinal tract. In recent years, lactic acid bacteria (LAB) have been verified to be a promising delivery vector for susceptible functional proteins drugs. KiSS-1 peptide, cancer suppressor, plays critical role inhibiting metastasis its activity has confirmed by direct administration. whether this...
(1) Background: Modern dietary patterns with a high intake of fat and fructose, as well refined carbohydrates, closely relate to lipid/glucose metabolic disorders. The main objective this study is provide new thoughts in designing functional food some metabolism regulating effects for obese people. (2) Methods: alleviating abilities γ-oryzanol, phytosterol or ferulic acid-enriched wheat flour on dysfunction were evaluated male SD rats induced by high-fat-fructose diet. underlying mechanisms...
Bioprospecting of more novel probiotic strains has attained continuous interest. This study aimed to investigate the beneficial effects Lactobacillus paracasei strain L14, an isolate from a traditional Chinese dairy product, on type 2 diabetes mellitus (T2DM) rats. Preventive supplementation L14 showed excellent anti-diabetic high-fat diet/low-dose streptozotocin (HFD/STZ)-induced T2DM It significantly reduced hyperglycemia, protected pancreatic β-cell and liver function, ameliorated...
Abstract Adhesion to host cells is considered important for Lactobacillus plantarum as well other lactic acid bacteria (LAB) persist in human gut and thus exert probiotic effects. Here, we sequenced the genome of Lt. strain NL42 originating from a traditional Chinese dairy product, performed comparative genomic analysis characterized novel adhesion factor. The was highly divergent its closest neighbors, especially six large regions. harbors total 42 genes encoding adhesion-associated...
Genome engineering is essential for application of synthetic biology in probiotics including lactobacilli and bifidobacteria. Several homologous recombination system-based mutagenesis tools have been developed these bacteria, but still many limitations different species or strains. Here we a genome method based on an inducible self-destruction plasmid delivering DNA into bacteria. Excision the replicon by induced recombinase facilitates selection events. This new editing tool called (IPSD)...
A case of Acinetobacter baumannii (A. baumannii), known as gram-negative bacteria, causes a range nosocomial infections. Due to the continuous detection multi-drug resistant A. in clinic, there is an urgent need find alternative therapies, including broad-spectrum antibacterial peptides (AMP). Recently it has been found that peptide Cec4 good activity against baumannii, but mechanism remains elusive.The basic structure was analyzed by circular dichroism (CD) spectroscopy, and potential...
Abstract Mesenchymal stromal/stem cells (MSC) have emerged as a promising therapeutic avenue for treating autoimmune diseases, eliciting considerable interest and discussion regarding their underlying mechanisms. This study revealed the distinctive ability of human umbilical cord MSC to aggregate within lymph nodes mice afflicted with but this phenomenon was not observed in healthy mice. The specific distribution is driven by heightened expression CCL21-CCR7 axis facilitating targeted homing...
Lactobacilli play an important role in the maintenance of a healthy vaginal microbiota, and some select species are widely used as probiotics. Vaginal isolates Lactobacillus gasseri DSM 14869 rhamnosus 14870 were previously selected to develop probiotic EcoVag capsules showed therapeutic effects women with bacterial vaginosis (BV). However, molecular mechanisms involved their activity largely unknown. In this study, we identified three cell surface molecules L. that promote adhesion...
The incretin hormones are extremely rapidly metabolized by the ubiquitous enzyme dipeptidyl peptidase IV (DPP-IV). Therefore, DPP-IV inhibitors which can prolong effect newest and promising drugs for management of type 2 diabetes. In this study, we investigated whether Bifidobacteria colonizing human gut possess inhibitory activity. Cell-free intracellular extracts 13 Bifidobacterium strains isolated from breast-fed infant faecal samples were prepared screened activity, two strains-Bif....
Exendin-4, a glucagon-like protein-1 (GLP-1) receptor agonist, is an excellent therapeutic peptide drug for type 2 diabetes due to longer lasting biological activity compared GLP-1. This study explored the feasibility of using probiotic Lactobacillus paracasei as oral vector recombinant exendin-4 delivery, alternative costly chemical synthesis and inconvenient administration by injection. L. transformed with plasmid encoding gene (L. L14/pMG76e-exendin-4) constitutive promotor was...
Abstract Human umbilical cord mesenchymal stem cells (hUC-MSCs) have shown potential as a therapeutic option for lupus nephritis (LN), particularly in patients refractory to conventional treatments. Despite extensive translational research on MSCs, the precise mechanisms by which MSCs migrate kidney and restore renal function remain incompletely understood. Here, we aim clarify spatiotemporal characteristics of hUC-MSC migration into LN kidneys their interactions with host microenvironment....
Objective of this paper is to excavate the siderophore synthesis gene from Brevibacillus brevis GZDF3 and verify its type antibacterial effects. The method using genome mining technology analyze genes phylogenetic tree each was constructed separately. Iron free medium utilized induce CAS liquid detection used for qualitative quantitative analysis on siderophore. preliminaries identified by Arnow effects were analyzed according agar punching method. results show that a cluster with 83%...