A5066262246- Plant Molecular Biology Research
- Transgenic Plants and Applications
- Plant nutrient uptake and metabolism
- Plant tissue culture and regeneration
- Plant Reproductive Biology
- Genetic Mapping and Diversity in Plants and Animals
- Legume Nitrogen Fixing Symbiosis
- Rice Cultivation and Yield Improvement
- Plant Virus Research Studies
- CRISPR and Genetic Engineering
- Plant Gene Expression Analysis
- Endoplasmic Reticulum Stress and Disease
- Chromosomal and Genetic Variations
- GABA and Rice Research
- Molecular Biology Techniques and Applications
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Genetically Modified Organisms Research
- Genetic Syndromes and Imprinting
- Photosynthetic Processes and Mechanisms
- Light effects on plants
- Biofuel production and bioconversion
- Autophagy in Disease and Therapy
- RNA regulation and disease
- Genomics and Phylogenetic Studies
Institute of Agrobiological Sciences
2014-2024
National Agriculture and Food Research Organization
2016-2024
Health First
2021
Salk Institute for Biological Studies
2016-2019
The University of Tokyo
2004-2009
Food Research Institute
2008
National Institute of Genetics
2004
Unlike animals, plants can pause their life cycle as dormant seeds. In both and DNA methylation is involved in the regulation of gene expression genome integrity. reprogramming erases re-establishes during development. However, knowledge or reconfiguration has been limited to pollen central cell. To better understand epigenetic embryo, which forms plant body, we compared time-series methylomes dry germinating seeds publicly available seed development methylomes. Time-series whole bisulfite...
Abstract Rice (Oryza sativa) seed storage proteins (SSPs) are synthesized and deposited in organelles the endosperm during maturation as a nitrogen source for germinating seedlings. We have generated glutelin, globulin, prolamin knockdown lines examined their effects on quality. A reduction of one or few SSP(s) was compensated by increases other SSPs at both mRNA protein levels. Especially, glutelins sulfur-rich 10-kD levels preferentially sulfur-poor prolamins, respectively, indicating that...
Summary The rice ( Oryza sativa L.) basic leucine Zipper factor RISBZ1 and prolamin box binding (RPBF) are transcriptional activators of seed storage protein (SSP) genes in vivo . To ascertain the functions these trans ‐activators development, knock‐down (KD) transgenic plants were generated which accumulation RPBF was reduced an endosperm‐specific manner by co‐suppression (KD‐RISBZ1 KD‐RPBF). most SSPs changed little between individual KD mutants wild‐type plants, whereas a double mutant...
During postembryonic development of higher plants, the shoot apical meristem produces lateral organs in a regular spacing (phyllotaxy) and timing (plastochron). Molecular analysis mutants associated with phyllotaxy plastochron would greatly increase understanding developmental mechanism plant architecture because are fundamental regulators architecture. pla1 rice is not only mutant showing rapid leaf initiation without affecting phyllotaxy, but also heterochronic ectopic formation...
A new glutelin gene, designated GluD-1, has been discovered by comparing the seed storage proteins from 48 japonica and indica rice cultivars on SDS-PAGE gels. Evidence that GluD-1 is a member of family was provided Western blots using anti-glutelin antiserum mapping gene to chromosomal cluster. The limited size polymorphism among varieties due amino acid substitutions rather than post-transcriptional modification. maximally expressed in starchy endosperm starting at 5 d after flowering...
Binding protein (BiP) is the key chaperone involved in folding of secretory proteins such as seed storage ER lumen. To obtain functional information about BiP1, a gene that predominantly expressed during rice maturation, we generated several transgenic plants which various levels BiP1 accumulated an endosperm-specific manner. Severe suppression (BiP1 KD) or significant over-expression OEmax) not only altered phenotype and intracellular structure endosperm cells, but also reduced content,...
The endoplasmic reticulum (ER) stress sensor IRE1 transduces signals by inducing the unconventional splicing of mRNAs encoding key transcription factors: HAC1 in yeast and XBP1 animals. However, no or homologues have been found plants, until recently substrate for plant has remained unknown. This study demonstrates that Oryza sativa (rice) OsbZIP50 factor, an orthologue Arabidopsis AtbZIP60, is regulated IRE1-mediated its RNA. Despite presence a transcriptional activation domain, protein not...
DNA cytosine methylation is an epigenetic mark associated with silencing of transposable elements (TEs) and heterochromatin formation. In plants, it occurs in three sequence contexts: CG, CHG, CHH (where H A, T, or C). The latter does not allow direct inheritance during replication due to lack symmetry, must therefore be re-established every cell generation. Genome-wide association studies (GWAS) have previously shown that CMT2 NRPE1 are major determinants genome-wide patterns TE...
Seed storage proteins are specifically and highly synthesized during seed maturation deposited into protein bodies (PBs) via the endoplasmic reticulum (ER) lumen. The accumulation process is mediated by ER chaperones such as luminal binding (BiP) disulfide isomerase (PDI). To examine role of relationship between levels proteins, we generated transgenic rice plants in which BiP PDI genes were overexpressed an endosperm-specific manner under control glutelin promoter. phenotype...
The endoplasmic reticulum (ER) responds to the accumulation of unfolded proteins in its lumen (ER stress) by activating intracellular signal transduction pathways. These pathways are known as ER stress response or protein response. In this study, three rice basic leucine zipper (bZIP) transcription factors (OsbZIP39, OsbZIP50 and OsbZIP60) containing putative transmembrane domains (TMDs) their C-terminal regions were identified candidates sensor transducer. One these proteins, OsbZIP39, was...
Chromatin modifications, such as cytosine methylation of DNA, play a significant role in mediating gene expression plants, which affects growth, development, and cell differentiation. As root hairs are single-cell extensions the epidermis primary organs for water uptake nutrients, we sought to use model system measure impact environmental stress. We measured changes DNA compared with multicellular stripped roots, well response heat Differentially methylated regions (DMRs) each context showed...
Abstract In higher plants, leaves initiate in constant spatial and temporal patterns. Although the pattern of leaf initiation is a key element plant shoot architecture, little known about how time interval between events, termed plastochron, regulated. Here, we present detailed analysis plastochron2 (pla2), rice (Oryza sativa) mutant that exhibits shortened plastochron precocious maturation during vegetative phase ectopic formation reproductive phase. The corresponding PLA2 gene revealed to...
Most aerial parts of the plant body are products continuous activity shoot apical meristem (SAM). Leaves major component body, and their temporal spatial distribution mainly determines architecture. Here we report identification rice gene PLASTOCHRON3 (PLA3)/GOLIATH (GO) that regulates various developmental processes including rate leaf initiation (the plastochron). PLA3/GO encodes a glutamate carboxypeptidase, which is thought to catabolize small acidic peptides produce signaling molecules....
Summary Separating male and female sex organs is one of the main strategies used to maintain genetic diversity within a species. However, determinants their regulatory mechanisms have been identified in only few In dioecious persimmons, homeodomain transcription factor, Me GI , which target Y chromosome‐encoded small‐ RNA OGI can determine floral sexuality. The basic features this system are conserved monoecious hexaploid Oriental persimmon, an additional epigenetic regulation determines...
Abstract In angiosperms, endosperm development comprises a series of developmental transitions controlled by genetic and epigenetic mechanisms that are initiated after double fertilization. Polycomb repressive complex 2 (PRC2) is key component these mediate histone H3 lysine 27 trimethylation (H3K27me3); the action PRC2 well described in Arabidopsis thaliana but remains uncertain cereals. this study, we demonstrate mutation rice (Oryza sativa) gene EMBRYONIC FLOWER2a (OsEMF2a), encoding...
Abstract In the evolutionary history of plants, variation in cis-regulatory elements (CREs) resulting diversification gene expression has played a central role driving evolution lineage-specific traits. However, it is difficult to predict behaviors from CRE patterns properly harness them, mainly because biological processes are complex. this study, we used cistrome datasets and explainable convolutional neural network (CNN) frameworks genome-wide tomato (Solanum lycopersicum) fruit DNA...
Lysine is the most defi cient essential amino acid in cereal grains.A bifunctional lysine-degrading enzyme, lysine ketoglutarate reductase/saccharopine dehydrogenase (LKR/ SDH), one of key regulators determining free content plants.In rice ( Oryza sativa .L), a OsLKR/SDH predominantly present seeds.Here, we show that directly regulated by major transcriptional seed storage protein (SSP) genes: basic leucine zipper (bZIP) transcription factor (TF), RISBZ1, and DNA-binding with fi nger (DOF)...
Summary Human IL‐10 (hIL‐10) is a therapeutic treatment candidate for inflammatory allergy and autoimmune diseases. Rice seed‐produced can be effectively delivered directly to gut‐associated lymphoreticular tissue (GALT) via bio‐encapsulation. Previously, the codon‐optimized hIL‐10 gene was expressed in transgenic rice with signal peptide endoplasmic reticulum (ER) retention (KDEL) at its 5′ 3′ ends, respectively, under control of endosperm‐specific glutelin GluB‐1 promoter. The resulting...
The accumulation of unfolded proteins in the endoplasmic reticulum (ER) lumen leads to ER stress. Intracellular signalling pathways are activated alleviate stress sensor IRE1 induces active form key transcription factors, such as XBP1 mammals and bZIP50 Oryza sativa (rice), by mediating unconventional splicing their mRNAs. Although characterization cis-elements that recognized these factors is essential for understanding responses, remain unidentified plants. Here, a cis-element named...
Abstract Plants remember their exposure to environmental changes and respond more effectively the next time they encounter a similar change by flexibly altering gene expression. Epigenetic mechanisms play crucial role in establishing such memory of fine-tuning With recent advancements biochemistry sequencing technologies, it has become possible characterize dynamics epigenetic on scales ranging from short term (minutes) long (generations). Here, our main focus is describing current...