In this study, an efficient natural deep eutectic solvent (NADES, 30 mL/100 mL water content) was identified and coupled with ultrasonication to extract phenolic compounds from broccoli leaves (BLs). A NADES prepared using choline chloride 1,2-propylene glycol (ChCl-PG) at a molar ratio of 1:2 provided the highest extraction rate. Phenolic conditions were optimized single-factor experiments response surface methodology. The optimal parameters as follows: solvent-to-solid ratio, 36.35 mL/g;...

This study primarily explored the internal mechanism underlying ultrasonication-induced release of antioxidant peptides. An oxhide gelatin solution was treated ultrasonically (power = 200, 300, and 400 W), followed by enzymatic hydrolysis structural morphological analysis. The results showed that ultrasonication increased not only degree (DH) protein recovery rate but also ABTS radical scavenging, DPPH ferrous chelating, ferric reducing activities its hydrolysate. hydrolysate with 300-W had...

The effects of low doses sodium nitrite on yak meat colouring, myoglobin oxygenation status, aggregation and structure were evaluated using Fourier transform infrared spectroscopy, laser micro-Raman spectroscopy liquid chromatography-electrospray ionization tandem mass spectrometry. results showed that the redness value increased steadily relative to control after addition dose nitrite. nitrosomyoglobin level gradually was significantly higher in nitrite-treated group than group. secondary...

Dual modification of collagen was performed using ionic liquid (IL) and ultrasound (US) to modulate the activity hydrolyzed peptides reveal production mechanism cowhide-derived dipeptidyl peptidase (DPP-IV) inhibitory peptides.The results revealed that dual (IL + US) significantly improved hydrolytic degree (P < 0.05). Meanwhile, IL US tended promote break hydrogen bonds, but inhibit crosslinking between collagens. The double reduced thermal stability accelerated exposure tyrosine...

To investigate the effect of gelatin peptide on inhibition quality deterioration in stored pudding, with antioxidant properties was added to pudding products. For this purpose, a recipe containing peptides created. The were characterized based their activity and protein structure. It found that had better properties, lower thermal stability crystallinity, higher hydrophobic amino acid content, greater surface hydrogen bond exposure than commercially available peptides. Properties such as pH,...

This study aimed to investigate the effect of Pyracantha fortuneana fruit extract (PFE) as a nitrite substitute on quality, flavor, contents biogenic amines and formation antioxidant peptides fermented beef sausages during storage. Compared with control group, treated PFE showed elevated L* a* values, enhanced activity, increased resistance microbial growth lipid protein oxidation. In particular, there were 2.44 ± 0.02 mg MDA/kg thiobarbituric acid reactive substances after 35 d....

In this study, we examined the role of mRNAs and miRNAs in variations intramuscular fat content longissimus dorsi muscle Xinjiang brown cattle. Two groups cattle with extremely different were selected for combined miRNA mRNA analysis using an RNA-Seq. total, 296 362 significantly differentially expressed, including 155 newly predicted miRNAs, 275 upregulated genes, 252 21 downregulated genes 110 miRNAs. The identified 96 expressed 27 mRNAs. all, 47 had a regulatory effect on 14 target 49 13...

Lipid oxidation and metmyoglobin (MMb) reduction are popular issues in meat color research. This study evaluated the effects of aldehyde products, particularly 4-hydroxy-2-nonenal (HNE) hexenal, lipid on oxymyoglobin stability, mitochondrial membrane permeability, MMb ability, electron transport chain-mediated reduction, nicotinamide adenine dinucleotide reduced form (NADH)-dependent reductase activity bovine Longissimus muscle. The results indicated that HNE hexenal accelerate rate...

Postmortem muscle moisture loss leads to a decrease in carcass weight and can adversely impact overall meat quality. Therefore, it is critical investigate water holding capacity (WHC) enhance Current research has primarily focused on examining the correlation between signaling molecules quality relation glycolysis effect WHC. But there exists significant knowledge gap regarding mechanism of WHC Jersey cattle-yak meat.

Despite the well-established individual roles of AMP-activated protein kinase (AMPK) and hypoxia-inducible factor-1α (HIF-1α) in sensing energy stress and/or hypoxia postmortem muscle, a knowledge gap persists regarding their interplay consequent impact on yak meat color stability. This study aimed to elucidate AMPK–HIF–1α interaction its consequential mitochondrial functionality, metabolism, ultimately, attributes. Postmortem longissimus thoracis et lumborum (LTL) muscles were incubated...

Purpose Social entrepreneurship, leveraging economic activities to achieve social benefits, offers women the opportunity freely and actively shape contours of their work in meaningful ways. This study aims examine how Chinese use job crafting entrepreneurship align gender identity, forge new relationships navigate mixed expectations. Design/methodology/approach The is based on 19 in-depth interviews with young engaging China. Using a grounded theory approach, explores craft identity into...

Abstract In this study, the feasibility of preparing oxhide gelatin from cowhide scrap by high pressure assisted‐liquid extraction was verified. Different processing conditions, including time (15 to 25 min), (250 350 MPa), and liquid‐to‐solid ratio (1:3 1:5), were optimized through response surface methodology. Under optimum manufacturing when high‐pressure (HPP) 22 min, 289 MPa, 1:4, highest yield (36%) gel strength (224 g) achieved. Based on DSC, XRD, FTIR, SEM, gelling melting...

The previous studies reported the antioxidant and anti-inflammatory properties of Schisandrin A (Sch A). This study aimed to investigate ability Sch protect against lung oxidative stress induced by combination cigarette smoke extract lipopolysaccharide (LPS) in an vitro model chronic obstructive pulmonary disease (COPD).The cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Colorimetry used detect changes markers. Quantitative...

Summary The antioxidant peptide was produced from hydrolysis with Bacillus subtilis proteases supplemented different duration of ultrasound, and the structural characteristics yak skin gelatin (YSG) were characterised. Compared excessive ultrasonic treatment (10–40 min), YSG by moderate (5 min) had a higher degree DPPH free radical scavenging ability, ABTS activity hydrolysate increased. results showed that ultrasound for 5 min significantly weakened hydrogen bonds promoted exposure...