A5101857403- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Acute Kidney Injury Research
- Cardiac Ischemia and Reperfusion
- Circular RNAs in diseases
- Cardiac Arrest and Resuscitation
- MicroRNA in disease regulation
- Inflammasome and immune disorders
- S100 Proteins and Annexins
- Cardiac Valve Diseases and Treatments
- Congenital Heart Disease Studies
- Ubiquitin and proteasome pathways
- Pulmonary Hypertension Research and Treatments
- Protease and Inhibitor Mechanisms
- Atherosclerosis and Cardiovascular Diseases
- Signaling Pathways in Disease
- Anesthesia and Neurotoxicity Research
- Cardiac Structural Anomalies and Repair
- Adipokines, Inflammation, and Metabolic Diseases
- Mechanical stress and fatigue analysis
- Aortic Disease and Treatment Approaches
- Inflammatory Biomarkers in Disease Prognosis
- Cancer-related gene regulation
- Cardiac and Coronary Surgery Techniques
- Immune Cell Function and Interaction
First Affiliated Hospital of Anhui Medical University
2014-2024
Anhui Medical University
2014-2024
Abstract Many studies have shown that long-noncoding RNA (lncRNA) is associated with cardiovascular disease, but its molecular mechanism still unclear. In this study, we explored the role of lncRNA ANRIL in ox-LDL-induced phenotypic transition human aortic smooth muscle cells (HASMC). The results quantitative fluorescence PCR showed expression patients coronary atherosclerotic heart disease (CAD) was significantly higher than normal subjects. RNA-FISH detection increased HASMC treated by...
RORγt is a key transcription factor that controls the development and function of inflammatory Th17. The mechanisms regulate stability remain unclear. We report Th17 cells highly express deubiquitinase ubiquitin-specific protease (USP)4, which essential for maintaining cell function. Inhibition catalytic activity USP4 with vialinin A, compound derived from Chinese traditional medicine, dampened differentiation. interacted deubiquitinated K48-linked polyubiquitination RORγt, thereby promoting...
Abstract The aim of the present study was to explore role long non-coding RNA (lncRNA) repressor NFAT (NRON) in atrial fibrosis and whether its underlying mechanism associated with macrophage polarization. Enzyme-linked immunosorbent assay (ELISA) analysis pro-inflammatory cytokines revealed that NRON overexpression suppressed, whereas silencing facilitated angiotensin II (Ang II)-induced inflammatory response primary cultured myocytes. chromatin immunoprecipitation (ChIP) results showed...
Objective To ascertain if levosimendan postconditioning can alleviate lung ischemia–reperfusion injury (LIRI) in rats. Method One hundred rats were divided into five groups: Sham (sham), group (I/R group), ischemic (IPO (Levo group) and combination of 5-Hydroxydecanoic acid (Levo+5-HD group). The apoptotic index (AI) tissue cells was determined using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Expression active cysteine aspartate specific protease-3 (...
Smoking is an important factor in the pathogenesis of chronic obstructive pulmonary disease (COPD), which commonly characterised by cellular senescence and inflammation. Recently, miR-200b has emerged as target to cure lung disease; however, function reducing inflammatory responses not been reported. In this study, we found that was downregulated lungs COPD model mice, its expression correlated with responses. We hypothesised may be a potential novel therapy for treating COPD. performed...
Nuclear factor of activated T cells (NFAT) is an important regulator cell activation. However, the molecular mechanism whereby NFATc2 regulates IL2 transcription not fully understood. In this study, we showed that ubiquitin‐specific protease 22 (USP22), known as a cancer stem marker, specifically interacted with and deubiquitinated NFATc2. USP22 stabilized protein levels, which required its deubiquitinase activity. Consistent these observations, depletion in reduced expression IL2, cytokine...
Atherosclerosis (AS) is a chronic progressive disease caused by injury and functional changes in vascular smooth muscle cells (VSMCs). Long non‑coding RNAs (lncRNAs) are pivotal regulators AS development. The present study aimed to explore the roles molecular mechanisms of lncRNA CTBP1‑AS2 progression. A dual‑luciferase reporter assay confirmed that miR‑195‑5p downstream target miRNA was increased AS. expression levels serums patients with human aorta or decreased, respectively, following...
Abstract Aims Long non-coding RNA (LncRNA) small nucleolar host gene 18 (SNHG18) has been widely implicated in cancers. However, little is known about its functional involvement vascular diseases. Herein, we attempted to explore a role for SNHG18 modulating smooth muscle cell (VSMC) contractile phenotype and injury-induced neointima formation. Methods results Analysis of single-cell sequencing transcriptomic datasets showed decreased levels injured atherosclerotic murine human arteries,...
ObjectiveThis study aimed to develop and validate several artificial intelligence (AI) models identify acute myocardial infarction (AMI) patients at an increased risk of kidney injury (AKI) during hospitalization.MethodsIncluded were diagnosed with AMI from the Medical Information Mart for Intensive Care (MIMIC) III IV databases. Two cohorts Changzhou Second People's Hospital Xuzhou Center used external validation models. Patients' demographics, vital signs, clinical characteristics,...
Abstract The occurrence of cardiac surgery-associated acute kidney injury (CSA-AKI) increases hospital stay and mortality. MicroRNAs has a crucial role in AKI. This objective the current study is to explore function hsa-miR-494-3p inflammatory response human tubular epithelial (HK2) cells with hypoxia/reoxygenation. According KDIGO standard, patients after surgery cardiopulmonary bypass were divided into two groups: AKI (n = 10) non-AKI 8). HK2 raised normal hypoxia/reoxygenation...
Lung cancer is the leading cause of cancer-related death globally. Ubiquitin modification plays a crucial role in regulation gene expression, and closely associated with pathogenesis. The aim our study was to clarify mechanisms action for HECT, C2 WW domain containing E3 ubiquitin protein ligase 1 (HECW1) non-small cell lung (NSCLC). Herein, we demonstrate that expression HECW1 significantly increased NSCLC lines tissues. Upregulation markedly enhanced proliferation cells, whereas...
Abstract Background Aortic dissection (AD) is a rare disease with severe morbidity and high mortality. Presently, the pathogenesis of aortic still not completely clear, studying its will have important clinical significance. Methods We downloaded 28 samples from Gene Expression Omnibus (GEO) database (Accession numbers: GSE147026 GSE190635), including 14 healthy controls (HC) samples. The Limma package was used to screen differentially expressed genes. StarBasev2.0 tool predict upstream...
We speculated that ubiquitin specific peptidase 4 (USP4) may deubiquitinate interferon regulatory factor (IRF4) and affect T helper type 2 (Th2) cell function. This study aimed to validate this hypothesis. Here, the interaction between USP4 IRF4 were analyzed by co-immunoprecipitation assay. The deubiquitin effect of on was Ni-NTA pull down Luciferase reporter gene constructs used analyze effects USP4, nuclear activated cell-2 (NFATc2) activation interleukin-4 (IL-4) promoter. Then, Th2...
Atherosclerosis is an inflammatory disease; monocytes and macrophages play important role in the progression of this disease. However, mechanisms are not fully understood yet. Nicotinamide phosphate transferase (NAMPT) rate limiting enzyme synthesis NAD, but extracellular NAMPT shows characteristics cytokines/adipokines, suggesting that it may be a link between metabolism inflammation. In study, we compared expression levels NAMPT/NAD+/Sirt1 signaling pathway as well NAMPT, CRP IL-6...
E3 ubiquitin ligase gene, WWP2 , is associated with acute kidney injury ( AKI ). This research was conducted to explore the role of in . cell model produced human renal proximal tubular epithelial line HK ‐2) by ischemia‐reperfusion IR ) injury. CCK8 and flow cytometry assay were performed influence overexpression on proliferation apoptosis ‐induced ‐2 cells. Quantitative real‐time PCR immunoblotting (IB) assess gene protein expression. Then, p53 ubiquitylation degradation estimated...
Objective: We aimed to: (1) explore the risk factors that affect prognosis of cardiac surgery-associated acute kidney injury (CS-AKI) in patients undergoing renal replacement therapy (RRT) and (2) investigate predictive value Acute Physiology Chronic Health Evaluation (APACHE) III score, Sequential Organ Failure Assessment (SOFA) Vasoactive-Inotropic Score (VIS) for mortality RRT. Methods: Data from who underwent surgery January 2015 through February 2021 were retrospectively reviewed to...