A5020175988- Bone Tissue Engineering Materials
- Mesenchymal stem cell research
- Orthopaedic implants and arthroplasty
- Bone fractures and treatments
- Musculoskeletal synovial abnormalities and treatments
- Dental Implant Techniques and Outcomes
- Orthopedic Surgery and Rehabilitation
- Knee injuries and reconstruction techniques
- Sarcoma Diagnosis and Treatment
- 3D Printing in Biomedical Research
- Total Knee Arthroplasty Outcomes
- Venous Thromboembolism Diagnosis and Management
- Hydrogels: synthesis, properties, applications
- Cell Adhesion Molecules Research
- TGF-β signaling in diseases
- Tissue Engineering and Regenerative Medicine
- Dental Anxiety and Anesthesia Techniques
- Bone Metabolism and Diseases
- Vascular Malformations and Hemangiomas
- Periodontal Regeneration and Treatments
- Cancer Cells and Metastasis
- Reconstructive Surgery and Microvascular Techniques
- Graphene and Nanomaterials Applications
- Wnt/β-catenin signaling in development and cancer
- Sports injuries and prevention
Kawasaki Hospital
2019
Nara City Hospital
2006-2018
Nara Medical University
2006-2017
National Hospital Organization
2008-2017
National Archives and Records Administration
2003-2015
National Institute of Advanced Industrial Science and Technology
2008-2012
Osaka Prefectural Medical Center
2001
We previously reported a new cell transplantation method in which mesenchymal stem cells (MSCs) were cultured as sheets. The MSC sheets showed high alkaline phosphatase (ALP) activities and osteocalcin (OC) contents. In the present study, we transplanted such by injection to assess whether injectable could form bone tissue at subcutaneous sites. At 4 weeks after injection, injected areas hard mass formation. Each consisted of newly formed bone, evaluated radiographic, histological gene...
To establish the methods of demonstrating early fixation metal implants to bone, one side a Cobalt-Chromium (CoCr) based alloy implant surface was seeded with rabbit marrow mesenchymal cells and other left unseeded. The were further cultured in presence ascorbic acid, β-glycerophosphate dexamethasone, resulting appearance osteoblasts bone matrix on surface. Thus, we succeeded generating tissue-engineered CoCr implant. then implanted defects. Three weeks after implantation, evaluations...
In clinical cases, many orthopaedists have been troubled with bone fragility, such as fractures after devitalization therapy for tumour, pathological and metastatic tumours. The aim of this study was to determine whether loss osteogenic capacity devitalized autologous bones can be rescued using cultured marrow-derived mesenchymal cells. A model produced from rat femur by irradiation three groups were prepared: intact bone, irradiated combined Each transplanted subcutaneously into a syngeneic...
The periosteum is a thin membrane that surrounds the outer surface of bones and participates in fracture healing. However, molecular signals trigger/initiate periosteal reaction are not well established. We fractured rat femoral bone at diaphysis fixed it with an intramedullary inserted wire, expression regenerating gene (Reg) I, which encodes tissue regeneration/growth factor, was analyzed. Neither bone/marrow nor muscle showed Reg I before or after fracture. By contrast, elevated fracture,...
Artificial bones made of β-tricalcium phosphate (β-TCP) combined with bone marrow-derived mesenchymal stromal cells (BM-MSCs) are used for effective reconstruction defects caused by genetic defects, traumatic injury, or surgical resection tumors. However, the selection constructs high osteogenic potential before implantation is challenging. The purpose this study was to determine whether calcium concentration in BM-MSC culture medium can be as a nondestructive and simple marker selecting...
To enhance early bonding of an alumina ceramic implant to bone, we evaluated a method seeding the surface with bone marrow mesenchymal cells that differentiated osteoblasts and matrix prior implantation. The usefulness was in Japanese white rabbits. In our study, test piece loaded by tissue engineering technique implanted into rabbit bones. Three weeks after procedure, evaluation mechanical histological examination were performed. Histological noncell-loaded surfaces showed no infiltration...
Mesenchymal stem cells (MSCs) are believed to be the cell of origin for most sarcomas including osteosarcoma and malignant fibrous histiocytoma (MFH/UPS). To identify signaling pathways involved in sarcoma pathogenesis, we compared gene expression profiles rat MFH with those syngeneic MSCs. Analysis genes that characterize MSCs such as CD44, CD105, CD73, CD90 showed higher sarcomas. Pathways focal adhesion, cytokine-cytokine receptors, extracellular matrix chemokines, Wnt were down-regulated...
Reconstruction of large bone defects after resection malignant musculoskeletal tumors is a significant challenge in orthopedic surgery. Extracorporeal autogenous irradiated grafting treatment option for reconstruction. However, nonunion often occurs because the osteogenic capacity lost by irradiation. In present study, we established an graft model rat femur to assess whether matrix cell sheets improve osteogenesis bone. Osteogenic were prepared from marrow-derived stromal cells and...
Allogenic bone grafting, a technique used in orthopaedic surgery, has several problems, including low osteogenic activity. To overcome the problem, this study aimed to determine whether vivo osteogenesis could be enhanced using allogenic irradiated grafts after seeding with autologous marrow-derived mesenchymal stem cells (BMSCs). The cylinders were extracted from ACI rats and sterilized by irradiation. Donor BMSCs obtained fresh Fischer 344 (F344) rat marrow cell culture. or without...
Synovial hemangioma of the knee is a rare benign tumor. Very rarely, growth bone affected by long-term neglect an intra-articular Our patient had not only various clinical symptoms but also dysplasia femoral bone. In this report, we aimed to raise awareness prevent disorders arising from unnoticed or untreated occurring within joint.Our was 41-year-old Japanese man who occasional discomfort in right since elementary school. Although he undergone radiography at several hospitals childhood, no...
Skeletal diseases, such as nonunion and osteonecrosis, are now treatable with tissue engineering techniques. Single cell sheets called osteogenic matrix (OMCSs) grown from cultured bone marrow-derived mesenchymal stem cells show high potential; however, long preparation times currently limit their clinical application. Here, we report a cryopreservation OMCS transplantation method that shortens time. Cryopreserved rat OMCSs were prepared using slow- rapid-freezing methods, thawed,...