A5040468096- CRISPR and Genetic Engineering
- NF-κB Signaling Pathways
- Biomedical Ethics and Regulation
- RNA Interference and Gene Delivery
- Research Data Management Practices
- Immune Response and Inflammation
- Health and Medical Research Impacts
- Scientific Computing and Data Management
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- Virus-based gene therapy research
- Heat shock proteins research
- T-cell and B-cell Immunology
- Cytokine Signaling Pathways and Interactions
- Innovation and Socioeconomic Development
- Biomedical and Engineering Education
- Melanoma and MAPK Pathways
- Meta-analysis and systematic reviews
- RNA regulation and disease
- Biotechnology and Related Fields
- Semantic Web and Ontologies
- Science, Research, and Medicine
- Orthopedic Surgery and Rehabilitation
- Pluripotent Stem Cells Research
- Mentoring and Academic Development
Addgene
2014-2021
Galena Biopharma (United States)
2009-2011
Phio Pharmaceuticals (United States)
2009-2011
Abbott Fund
2001-2009
Abbott (United States)
2001-2005
University of Massachusetts Chan Medical School
2005
Massachusetts General Hospital
1990-1991
Harvard University
1990
Interleukin-1β converting enzyme (ICE) is a cytoplasmic cysteine protease required for generating the bioactive form of interleukin-1β cytokine from its inactive precursor. We report identification ICH-2, novel human gene encoding member ICE family, and characterization protein product. ICH-2 mRNA widely expressed in tissues pattern similar to, but distinct from, that ICE. Overexpression insect cells induces apoptosis. Purified functional as vitro. A comparison inhibitor profiles substrate...
Previous work has demonstrated that SLP-76, a Grb2-associated tyrosine-phosphorylated protein, augments Interleukin-2 promoter activity when overexpressed in the Jurkat T cell line. This requires regions of SLP-76 mediate protein-protein interactions with other molecules cells, suggesting SLP-76-associated proteins also function to regulate signal transduction. Here we describe molecular cloning SLAP-130, phosphoprotein 130 kDa. We demonstrate SLAP-130 is hematopoietic cell-specific and...
The Addgene Repository (http://www.addgene.org) was founded to accelerate research and discovery by improving access useful, high-quality materials information. repository archives plasmids generated scientists, conducts quality control, annotates the associated data makes their available scientific community. Plasmid undergoes ongoing curation members of community scientists. growing database contains information on >31 000 unique spanning most experimental biological systems organisms....
Signal transducer and activator of transcription (STAT) proteins are constitutively activated in many malignancies, including squamous cell carcinoma the head neck (SCCHN). Previously, we reported that phosphorylation epidermal growth factor receptor (EGFR) is linked to activation STATs 3 5 SCCHN cells. The present study was undertaken determine role Src family kinases STAT growth. c-Src, c-Yes, Fyn, Lyn were expressed by transforming factor-α stimulation all four lines examined but not...
Abstract Head and neck squamous cell carcinomas (HNSCCs) are characterized by up-regulation of the epidermal growth factor receptor (EGFR). We previously reported that a gastrin-releasing peptide/gastrin-releasing peptide (GRP/GRPR) autocrine pathway is activated early in HNSCC carcinogenesis. GRP can induce rapid phosphorylation EGFR p42/44 mitogen-activated protein kinase (MAPK) activation part via extracellular release transforming α (TGF-α) matrix metalloproteinases (MMPs). It has been...
The mechanism by which the products of v-rel oncogene, corresponding c-rel proto-oncogene, and related dorsal gene Drosophila melanogaster exert their effects is not clear. Here we show that v-rel, chicken c-rel, proteins activated expression when fused to LexA sequences bound DNA upstream target genes in Saccharomyces cerevisiae. We have defined two distinct activation regions protein. Region I, located amino-terminal half rel proteins, contains no stretches glutamines, prolines, or acidic...
The p105 precursor protein of NF-κB1 acts as an NF-κB inhibitory protein, retaining associated Rel subunits in the cytoplasm unstimulated cells. Tumor necrosis factor α (TNFα) and interleukin-1α (IL-1α) stimulate degradation, releasing to translocate into nucleus. By using knockout embryonic fibroblasts, it was first established that IκB kinase (IKK) complex is essential for these pro-inflammatory cytokines trigger efficiently degradation. PEST domain contains a motif...
Genome editing using clustered regularly interspersed short palindromic repeats (CRISPR) and CRISPR-associated proteins offers the potential to facilitate safe effective treatment of genetic diseases refractory other types intervention. Here, we identify some major challenges for clinicians, regulators, human research ethics committees in clinical translation CRISPR-mediated somatic cell therapy.
The t(15;17) translocation, found in 95% of acute promyelocytic leukemia, encodes a leukemia (PML)-retinoic acid receptor alpha (RARalpha) fusion protein. Complete remission can be obtained by treating patients with all-trans retinoic acid, and PML-RARalpha plays major role mediating effects cells. A main model proposed for is that exerts its oncogenic repressing the expression acid-inducible genes critical to myeloid differentiation. By applying subtraction cloning cells, we identified...
The mechanism by which the products of v-rel oncogene, corresponding c-rel proto-oncogene, and related dorsal gene Drosophila melanogaster exert their effects is not clear. Here we show that v-rel, chicken c-rel, proteins activated expression when fused to LexA sequences bound DNA upstream target genes in Saccharomyces cerevisiae. We have defined two distinct activation regions protein. Region I, located amino-terminal half rel proteins, contains no stretches glutamines, prolines, or acidic...
Chemical modification of RNA duplexes can provide practical advantages for interference (RNAi) triggering molecules including increased stability, safety and specificity. The impact nucleotide modifications on Dicer processing, RISC loading RNAi-mediated mRNA cleavage was investigated with >or=25 bp in length. It is known that dsRNAs are processed by to create classic 19-bp siRNAs 3'-end overhangs. We demonstrate the presence minimal configurations longer block processing result full-length...
Whereas biological materials were once transferred freely, there has been a marked shift in the formalisation of exchanges involving these materials, primarily through use Material Transfer Agreements (MTAs). This paper considers how risk aversion dominates MTA negotiations and impact it may have on scientific progress. Risk is often based unwarranted fears incurring liability material or loss control missing out commercialisation opportunities. Evidence to date suggested that complexity...
Addgene (www.addgene.org) is a nonprofit organization that facilitates biomedical research and discovery by improving access to useful materials information. To fulfill this mission, works with hundreds of laboratories all over the world collect high-quality published plasmids data for repository can then be distributed academic institutions used further research. Biological resource centers such as are an important part scientific infrastructure. They play key role in helping scientists...
Open and reproducible research practices increase the reusability impact of scientific research. The reproducibility results is influenced by many factors, most which can be addressed improved education training. Here we describe how workshops developed Reproducibility for Everyone (R4E) initiative customized to provide researchers at all career stages across disciplines with training in practices. R4E initiative, led volunteers, has reached more than 3000 worldwide date, workshop materials,...
Abstract Cell-type-specific expression of molecular tools and sensors is critical to construct circuit diagrams investigate the activity function neurons within nervous system. Strategies for targeted manipulation include combinations classical genetic such as Cre/loxP Flp/FRT, use cis-regulatory elements, knock-in transgenic mice, gene delivery by AAV other viral vectors. The combination these complex technologies with goal precise neuronal targeting a challenge in lab. This report will...
RNA interference (RNAi) has been established as an important tool for functional genomics studies and great promise a therapeutic intervention human diseases. In mammalian cells, RNAi is conventionally induced by 19-27-bp duplexes generated hybridization of two complementary oligonucleotide strands (oligos). Here we describe novel class molecules composed single 25-28-nucleotide (nt) oligo. The oligo 16-nt mRNA targeting region, followed additional 8-10 nt to enable self-dimerization into...