A5038639669- Advanced biosensing and bioanalysis techniques
- Electrochemical sensors and biosensors
- Biosensors and Analytical Detection
- Electrochemical Analysis and Applications
- SARS-CoV-2 detection and testing
- Molecular Junctions and Nanostructures
- Alzheimer's disease research and treatments
- Conducting polymers and applications
- Mosquito-borne diseases and control
- Molecular Sensors and Ion Detection
- Advanced Nanomaterials in Catalysis
- SARS-CoV-2 and COVID-19 Research
- RNA Interference and Gene Delivery
- Advanced Chemical Sensor Technologies
- Gold and Silver Nanoparticles Synthesis and Applications
- Lipid Membrane Structure and Behavior
- Supramolecular Self-Assembly in Materials
- RNA and protein synthesis mechanisms
- Analytical Chemistry and Sensors
- DNA and Nucleic Acid Chemistry
- Malaria Research and Control
- Plasmonic and Surface Plasmon Research
- Complement system in diseases
- MXene and MAX Phase Materials
- 2D Materials and Applications
Forschungszentrum Jülich
2017-2025
Ernst Ruska Centre
2023-2024
RWTH Aachen University
2019-2022
Zero to Three
2020
BioElectronics (United States)
2020
Stadtwerke Jülich (Germany)
2020
FH Aachen
2017
With the goal of fast and accurate diagnosis infectious diseases, this study presents a novel electrochemical biosensor that employs refined aptamer (C9t) for detection spike (S) protein SARS-CoV-2 variants in flexible multielectrode aptasensor array with PoC capabilities. Two modifications were employed: removing primer binding sites including two dithiol phosphoramidite anchor molecules. Thus, reducing fabrication time from 24 to 3 h increasing stability sparseness multi-thiol aptasensors...
Amyloid-β oligomers (AβO) have become representative biomarkers for early diagnosis of Alzheimer's disease. Here, we report on an aptasensor based stem-loop probes sensitive and specific detection AβO by amperometric transducer principle using alternating current voltammetry (ACV). Stem-loop with redox-active moieties are immobilized a gold substrate as receptor element. The signal transduction mechanism relies redox ferrocene (Fc) reporting via charge transfer molecular recognition event...
A dual-aptamer biosensor based on 3D nanostructured gold deposited multielectrode arrays (3D-GMEAs) was developed for the simultaneous detection of AβO and ATP in artificial cerebrospinal fluid.
Abstract Biosensors based on DNA aptamer receptors are increasingly used in diagnostic applications. To improve the sensitivity and specificity of aptasensors, parameters affecting stability binding efficiency receptor layer need to be identified studied. For example, blocking step, i.e., addition inert molecules layer, can sensor performance, but also cause phase separation into nanodomains unknown composition structure. Here, nano‐IR spectroscopy is together with complementary macroscopic...
Diabetes patients suffer either from insulin deficiency or resistance with a high risk of severe long-term complications, thus the quantitative assessment level is highly desired for diabetes surveillance and management. Utilizing insulin-capturing aptamers may facilitate development affordable biosensors however, their rigid G-quadruplex structures impair conformational changes diminish sensor signals. Here we report on ratiometric, electrochemical aptasensor which achieved by hybridization...
In this work, we combined plasmon-enhanced fluorescence and electrochemical (PEF-EC) transduction mechanisms to realize a highly sensitive dual-transducer aptasensor. To implement two traducers in one biosensor, novel large-scale nanoimprint lithography process was introduced fabricate gold nanopit arrays (AuNpA) with unique fringe structures. Light transmitting through the AuNpA samples exhibited surface plasmon polariton peak overlapping excitation of C7 aptamer-associated fluorophore...
Accurate determination of serotonin (ST) provides insight into neurological processes and enables applications in clinical diagnostics brain diseases. Herein, we present an electrochemical aptasensor based on truncated DNA aptamers a polyethylene glycol (PEG) molecule-functionalized sensing interface for highly sensitive selective ST detection. The have small size adopt stable stem-loop configuration, which improves the accessibility aptamer analyte enhances sensitivity aptasensor. Upon...
Abstract Two transducer principles are combined in one aptamer biosensor (aptasensor) by simultaneously performing electrochemical impedance spectroscopy (EIS) and surface plasmon polariton (SPP) detection of a malaria biomarker. A thin gold film perforated with nanohole arrays is modified small highly charged receptors utilized for the Plasmodium falciparum lactate dehydrogenase (PfLDH), main biomarker malaria. Monitoring same analyte binding events two independent transduction not only...
In this work, a novel sandwich-type electrochemical immunosensor was developed for the quantitative detection of carcinoembryonic antigen, an important tumor marker in clinical tests. The capture antibodies were immobilized on surface gold disk electrode, while attached to redox-tagged single-walled carbon nanohorns/thionine/AuNPs. Both types antibody immobilization carried out through Au-S bonds using photochemical technique that ensures control over orientation antibodies. electroactive...
Diabetes patients suffer either from insulin deficiency or resistance with a high risk of severe long-term complications, thus the quantitative assessment level is highly desired for diabetes surveillance and management. Here we report on ratiometric, electrochemical aptasensor which achieved by hybridization an insulin-capturing aptamer partially complementary ssDNA tagged anthraquinone (AQ) methylene blue (MB) redox probes, respectively. To improve durability aptasensor, capturing was...
Electrochemical and optical platforms are commonly employed in designing biosensors. However, one signal readout can easily lead to inaccuracies due the effect of nonstandard test procedures, different operators, experimental environments. We have developed a dual-signal protocol that combined two transducer principles aptamer-based biosensor by simultaneously performing electrochemical- extraordinary transmission (EOT)-based plasmonic detection using gold nanopit arrays (AuNpA). Compared...
Considering challenges for fast and accurate diagnosis of infectious diseases, this study presents a novel electrochemical biosensor that employs refined aptamer (C9t) the detection spike (S) protein SARS-CoV-2 variants in flexible multielectrode aptasensor array with PoC capabilities. Two modifications were employed: removal primer binding sites inclu-sion two dithiol phosphoramidite anchor molecules. Thus, reducing fabrication time from 24 to 3 hours multi-thiol aptasensors compared...
Assessment tests for glycemic control of patients with diabetes mellitus commonly use blood glucose and glycated hemoglobin A1C (HbA1C) levels estimating acute long-term glycemia, respectively. Diabetes diet, as well treatment, are adapted accordingly also the risk diabetic complications can be estimated. Glycated human serum albumin (GHSA) has been proposed an alternative to HbA1C since it possesses a four times shorter lifespan its concentration is not affected by hemic diseases. Here,...
Organic electrochemical transistors (OECTs) possess versatile advantages for biochemical and electrophysiological applications due to gating ion-to-electron conversion capability. Although OECTs have been successfully applied sensing, the effect of relative capacitance specific sensing events is still unclear. In present work, we design integrated interdigitated (iOECTs) with on-plane gold gate different channel geometries point-of-care diagnosis malaria using aptamer as receptor. The...