A5100425627- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Immune Cell Function and Interaction
- Virology and Viral Diseases
- Vector-borne infectious diseases
- Text and Document Classification Technologies
- Rabies epidemiology and control
- Advanced Sensor and Control Systems
- T-cell and B-cell Immunology
- HIV, Drug Use, Sexual Risk
- Immunotherapy and Immune Responses
- Malaria Research and Control
- Sentiment Analysis and Opinion Mining
- Topic Modeling
- Hydraulic and Pneumatic Systems
- Immune responses and vaccinations
- Color perception and design
- Neuroinflammation and Neurodegeneration Mechanisms
- Viral Infections and Outbreaks Research
- Advanced Combustion Engine Technologies
- Polymer-Based Agricultural Enhancements
- Textile materials and evaluations
Bloomberg (United States)
2024
Sidney Kimmel Comprehensive Cancer Center
2024
Johns Hopkins University
2024
Shanghai Public Health Clinical Center
2023
Kunming University of Science and Technology
2011-2022
First People's Hospital of Yunnan Province
2022
National Medical Products Administration
2016
Shenzhen Institutes of Advanced Technology
2015
Chinese Academy of Sciences
2015
Anhui Medical University
2014
Human APOBEC3G (hA3G) has been identified as an anti-HIV-1 host factor. The presence of hA3G in HIV-1 strongly inhibits the ability virus to produce new viral DNA upon infection. In this report, we demonstrate that reduction late synthesis is due inhibition by strand transfer steps occur during reverse transcription. Analysis cDNA intermediates vivo reveals causes minus and plus transfers, without having a significant impact on elongation. Using vitro system measure similarly shows...
Background Rabies is a lethal zoonotic disease that kills approximately 60,000 people each year. Although inactivated rabies vaccines are available, multiple-dose regimensare recommended for pre-exposure prophylaxis or post-exposure prophylaxis,which cuts down the cost- and time-effectiveness, especially in low- middle incomecountries. Methods We developed nucleoside-modified mRNA-lipid nanoparticle vaccine (RABV-G mRNA-LNP) encoding codon-optimized viral glycoprotein assessed immunogenicity...
ABSTRACT Factors that influence viability and function of cryopreserved peripheral blood mononuclear cells (PBMC) were identified on 54 samples from 27 AIDS Clinical Trial Units. PBMC ranged 1 to 96% with a median 70%, was higher in laboratories experienced staff, not significantly associated CD4 cell number. Function PBMC, measured by lymphocyte proliferation, viability. Preparations greater than or equal 70% had consistent proliferative responses suitable for functional analyses.
Zika virus (ZIKV) is an emerging mosquito-transmitted flavivirus that can cause severe disease, including congenital birth defect and Guillain−Barré syndrome during pregnancy. Although, several molecular diagnostic methods have been developed to detect the ZIKV, these pose challenges as they cannot early viral infection. Furthermore, require extraction of RNA, which easy contaminate. Nonstructural protein 1 (NS1) important biomarker for diagnosis virus, detection associated with NS1 recently...
An outbreak of dengue virus (DENV) occurred in Yunnan province. More than 2,000 individuals were infected from August to November 2013. In this study, we aimed characterize the origin and prevalence DENV Dehong prefecture province using phylogenetic evolutionary analyses strains collected local patients foreign travelers.A total 41 DENV-positive serum samples randomly travelers who entered China at Ruili port or with fever province, China. The envelope (E) gene was amplified sequenced....
Zika virus (ZIKV) is a mosquito-borne flavivirus that caused the public health emergency. Recently, we have proved novel small animal tree shrew was susceptive to ZIKV infection and presented most common rash symptoms as patients. Here further cultured primary cells from different tissues of this determine tissue tropism in vitro. The results showed kidney, lung, liver, skin aorta were permissive could support viral replication by detection specific RNA intra- extra-cells. In comparing,...
Animal models of Zika virus (ZIKV) infection have recently been established in mice, guinea pigs, and nonhuman primates. Tree shrews (Tupaia belangeri) are an emerging experimental animal biomedical applications, but their susceptibility to ZIKV has not explored. In the present study, we show that subcutaneous inoculation led rapid viremia viral secretion saliva, as well typical dermatological manifestations characterized by massive diffuse skin rash on trunk. Global transcriptomic...
Due to the open policy of Chinese government, a large number Burmese individuals enter China at land ports in Yunnan province for travel or business. However, situation HIV‐1 infection and its phylogenetic characteristics among these travelers remains unclear, which is potential threat public health. From January 2003 December 2012, total 1,961 were detected be positive between Myanmar province, China. 1153 (58.8%) them, we randomly collected 489 serum samples subtype/recombinant analysis....
Human immunodeficiency viruses (HIV)-induced cell fusion is a critical pathway of HIV spread from infected cells to uninfected cells. A rapid and simple assay was established measure HIV-induce fusion. This study particularly useful screen for inhibitors that block cell-to-cell transmission. Present demonstrated coculture HIV-infected with at 37 degree(s)C 2 hours resulted in the highest rate. Using this assay, we have identified several potent targeted gp41 core. These antiviral agents can...
Xishuangbanna is a popular tourist destination in southern Yunnan Province, bordering with Vietnam and Laos, Myanmar. There are more than 2 million cross‐border travelers annually entering China at two land ports, Daluo Mohan. To clarify the characteristics of HIV‐1 prevalence among entry travelers, retrospective epidemic investigation was conducted. Between 2003 2012, 196 91 432 were detected to be HIV‐1‐positive, accounting for infection rate 0.21%. Eighty‐one gag‐pol gene sequences...
Sexual transmission of Zika Virus (ZIKV) elevates the risk its dissemination in female reproductive tract and causes a serious threat to fetus. However, available animal models are not appropriate investigate sexual transmission, dynamics ZIKV infection, replication, shedding. The use tree shrew as small model vaginal infection was assessed this study. A total 23 sexually mature shrews were infected with GZ01 via intravaginal route. There no significant difference change body weight,...
Background: The outbreak of Zika virus (ZIKV) in many countries caused alarming numbers babies born with microcephalus and severe neurologic disorders, however, the methods for detection ZIKV are limited at present. Objectives: aim this study was to produce polyclonal antibody against full length envelop protein (ZIKV-E protein) prokaryotic system evaluate its efficacy capture virion. Methods: recombinant ZIKV-E purified then used as an immunogen vaccinate BALB/c mice antibody. Protein A/G...
The paper addresses the problem of flexible Workflow Management Systems (WFMS) in distributed environment. Concerning serious deficiency flexibility current workflow systems, we describe how our system meets requirements interoperability, scalability, flexibility, dependability and adaptability. With an additional route engine, execution path will be adjusted dynamically according to conditions so as improve system. A dynamic register mechanism domain engines is introduced scalability...
This paper puts forward the structure of water hydraulic overflow valve with Giant Magnetostrictive Actuator. In condition ignoring magnetic leakage and force uneven, we deduced static output displacement equation GMA. By establishing a mathematical model, get dynamic response time in rated electricity flowing down simulation. GMA has simple structure, good linear, enough displacement, high speed other significant advantages.