A5001086879- Plant Stress Responses and Tolerance
- Biosensors and Analytical Detection
- Rabies epidemiology and control
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Plant Molecular Biology Research
- Viral Infections and Outbreaks Research
- Virology and Viral Diseases
- Plant Gene Expression Analysis
- Poxvirus research and outbreaks
- Monoclonal and Polyclonal Antibodies Research
- Advanced biosensing and bioanalysis techniques
- Bacteriophages and microbial interactions
- Malaria Research and Control
- Plant Reproductive Biology
- Plant Micronutrient Interactions and Effects
- Salmonella and Campylobacter epidemiology
- Antimicrobial Peptides and Activities
- Virus-based gene therapy research
- Vibrio bacteria research studies
- Escherichia coli research studies
- Plant nutrient uptake and metabolism
- Hepatitis B Virus Studies
- Antibiotic Resistance in Bacteria
- Aluminum toxicity and tolerance in plants and animals
Kunming University of Science and Technology
2016-2025
Soochow University
2024
Shanghai Electric Cable Research Institute
2019
Second People's Hospital of Yunnan Province
2019
Huazhong Agricultural University
2011-2012
Plant microRNAs (miRNAs) are vital components of the translation control system that regulates plant development and reproduction. The biological function sly-miR156 was investigated by over-expression in tomato plants. Transgenic plants exhibited a drastically altered phenotype, with reduced height, smaller but more numerous leaves, fruit. inflorescence structure over-expressing phenocopied sft mutant. putative targets were identified data base search included six SQUAMOSA PROMOTER BINDING...
Hepatitis B virus (HBV) infection is a global public health problem that closely related to liver cirrhosis and hepatocellular carcinoma (HCC). The prevalence of acute chronic HBV infection, cirrhosis, HCC has significantly decreased as result the introduction universal vaccination programs. first hepatitis vaccine approved was developed by purifying surface antigen (HBsAg) from plasma asymptomatic HBsAg carriers. Subsequently, recombinant DNA technology led development vaccine. Although...
Wild species often show more tolerance to environmental stress factors than their cultivated counterparts. An early responsive-to-dehydration gene was cloned from a drought- and salt-tolerant wild tomato Solanum pennellii (SpERD15). SpERD15 transcript accumulated differentially in different organs, remarkably induced by dehydration, salinity, cold treatment with plant growth regulators. The protein encoded predominantly localized the nucleus. Interestingly, we found that majority of...
Background and Aims Aluminium (Al) toxicity is a limiting factor for plant growth crop production in acidic soils. Citrate exudation activation of the plasma membrane H+-ATPase are involved soybean responses to Al stress. Auxin has crucial functions stress responses. However, little known about possible interactions between auxin citrate under In this study, we elucidated regulatory roles IAA Al-induced roots. Methods We measured content, concentration, exudation, activity, expression...
Shigella is an important human food-borne zoonosis bacterial pathogen, and can cause clinically severe diarrhea. There urgent need to develop a specific, sensitive rapid methodology for detection of this pathogen. In study, loop-mediated isothermal amplification (LAMP) combined with magnetic immunocapture assay(IC-LAMP) was first developed the in pure culture, artificial milk, clinical stool samples. This method exhibited limit 8.7 CFU/mL. Compared PCR, IC-LAMP sensitive, reliable monitoring...
Zika virus (ZIKV) is an emerging mosquito-transmitted flavivirus that can cause severe disease, including congenital birth defect and Guillain−Barré syndrome during pregnancy. Although, several molecular diagnostic methods have been developed to detect the ZIKV, these pose challenges as they cannot early viral infection. Furthermore, require extraction of RNA, which easy contaminate. Nonstructural protein 1 (NS1) important biomarker for diagnosis virus, detection associated with NS1 recently...
Antibiotic resistance has emerged as a grave threat to global public health, leading an increasing number of treatment failures. Antimicrobial peptides (AMPs) are widely regarded potential substitutes for traditional antibiotics since they less likely induce when used. A novel AMP named Brevinin-1BW (FLPLLAGLAASFLPTIFCKISRKC) was obtained by the Research Center Molecular Medicine Yunnan Province from skin Pelophylax nigromaculatus. Brevinia-1BW had effective inhibitory effects on...
Data hiding unnoticeably conceals message to prevent such from being leaked the existing media so as ensure confidentiality of relevant information, which plays an important role in DNA cryptography. A data model based on sequence and recombinant technique has been proposed transmit information securely. According model, a can be hidden by using single mapping rule table, further concealed plasmid technique. The recombined organisms screened selectable markers. This mechanism improve...
SummaryPlant seeds contain the sporophytic embryo and nutrients for development. To ensure correct seed formation, co-ordination between embryo, endosperm, maternal components must be achieved. However, this regulatory mechanism is poorly understood. Here, we demonstrate that an NAC gene (SlNAC3) was essential young endosperm development in tomato (Solanum lycopersicum). Transgenic expressing SlNAC3 RNA interference (RNAi) exhibited defects pollen viability a reduced size. Ultrastructural...
More than 250 million people worldwide have chronic hepatitis B virus (HBV) infections, resulting in over 1 annual fatalities because HBV cannot be adequately treated with current antivirals. Hepatocellular carcinoma (HCC) risk is elevated the presence of HBV. Novel and powerful medications that specifically target persistent viral components are needed to remove infection. This study aimed use HepG2.2.15 cells rAAV-HBV1.3 C57BL/6 mouse model established our laboratory examine effects 16F16...
Klebsiella pneumoniae is a common human pathogen that causes several nosocomial chronic infections. Many methods have been reported to detect pneumonia; however, simple, rapid, and visual method remains be developed. Here, we developed detection employing nanoparticle immunosensor (NPIS) with sensitivity of 8 CFU mL-1. Two monoclonal antibodies (mAbs) recognizing different antigenic determinants on the surface pneumonia were screened by an indirect enzyme-linked immunosorbent assay (ELISA),...
Japanese encephalitis virus (JEV), a member of the Flaviviridae family and flavivirus, is known to induce acute encephalitis. Vimentin protein has been identified as potential receptor for JEV, engaging in interactions with viral membrane protein. The Fc fragment, an integral constituent immunoglobulins, plays crucial role antigen recognition by dendritic cells (DCs) or phagocytes, leading subsequent presentation, cytotoxicity, phagocytosis. In this study, we fused JEV vimentin fragment IgG...
SummaryThe NAC (acronym derived from the names of first three cloned genes NAM, ATAF1/ATAF2, and CUC) transcription factor family is important for plant development environmental stress responses. We isolated NtNAC2, a new salt-inducible gene in tobacco (Nicotiana tabacum var. Xanthi). NtNAC2 encoded 311 amino acids deduced protein sequence contained five typical domains. Most secondary structure predicted was made up αhelices βsheets. analysed tissue-specific expression profiles found that...
Rabies virus is a highly neurophilic negative-strand RNA with high lethality and remains huge public health problem in developing countries to date. The double-stranded RNA-binding protein Staufen1 (STAU1) has multiple functions replication, transcription, translation. However, its function RABV infection mechanism of action are not clear. In this study, we investigated the role host factor STAU1 SH-SY-5Y cells. Immunofluorescence, TCID50 titers, confocal microscopy, quantitative real-time...
Rabies virus (RABV) is one of the old deadly zoonotic viruses. It attacks central nervous system and causes acute encephalitis in humans animals. Host factors are known to be essential for infection replication cells. The identification key host required RABV may provide important information on new potential targets drug discovery.This study aimed investigate change subcellular distribution expression protein Prefoldin subunit 1 (PFDN1) RABV-infected cells viral plasmids transfected...
In recent years, food safety incidents caused by Escherichia coli have occurred and endangered human health. Due to the complex matrix of milk samples long pretreatment time, existing methods cannot quickly detect E. in samples. It is necessary enrich improve detection sensitivity. The outer membrane protein A (OmpA) widely present on cell may be used as a new target coli. this study, purified recombinant OmpA was immunize BALB/c mice produce polyclonal antibody. Immunomagnetic beads were...
Rabies virus (RABV) is a deadly neurotropic that causes the disease of rabies in humans and animals. L protein one large structural virus, which displays multiple enzymatic activities, required for viral transcription replication.A truncated being cloned, expressed purified to produce relevant polyclonal antibody.The gene fragment RABV was subcloned into prokaryotic expression vector pET- 28a transformed E. coli Rosetta DE3 host strain. The recombinant characterized by SDS-PAGE western blot...
Abstract NAC (for N AM , A TAF1-2 and C UC2 ) proteins are one of the largest transcription factor families in plants. They have various functions closely related to developmental processes fruits. Tomato ( Solanum lycopersicum is a model plant for studies fruit growth patterns. In this study, functional characteristics action mechanisms new NAC-type factor, SlNAC3 (SGN-U568609), were examined determine its role tomato development ripening. The protein was produced by prokaryotic expression...