Abstract Background The multifaceted non-pharmaceutical interventions (NPIs) taken during the COVID-19 pandemic not only decrease spreading of SARS-CoV-2, but have impact on prevalence other viruses. This study aimed to explore common respiratory viruses among hospitalized children with lower tract infections (LRTI) in China pandemic. Methods Respiratory specimens were obtained from LRTI at Children’s Hospital Fudan University for detection syncytial virus (RSV), adenovirus (ADV),...

Abstract Nonpharmaceutical interventions (NPIs) taken to combat the coronavirus disease 2019 (COVID‐19) pandemic have not only decreased spread of severe acute respiratory syndrome 2 but also had an impact on prevalence other common viruses. This study aimed investigate long‐term NPIs and enteric viruses among children in Shanghai, China, as were relaxed after June 2020. The laboratory results clinical data outpatient with tract infections (ARTI) gastroenteritis (AGE) analyzed compared...

Respiratory syncytial virus (RSV) is the most common pathogen causing acute lower respiratory tract infection (LRTI) in children. RSV usually peaks winter and declines by early spring China. The outbreak of coronavirus disease 2019 (COVID-19) was reported to bring changes transmission pattern pathogens including RSV. Here this paper, we analyzed RSV-positive nasopharyngeal aspirates from inpatients Children's Hospital Fudan University October 2021 compared clinical features patients before...

China has adhered to policies of zero-COVID for almost three years since the outbreak COVID-19, which remarkably affected circulation respiratory pathogens. However, begun end in late 2022. Here, we reported a resurgence common viruses and Mycoplasma pneumoniae with unique epidemiological characteristics among children after ending policy Shanghai, China, 2023. Children hospitalized acute tract infections were enrolled from January 2022 December Nine 2 atypical bacteria detected specimens...

This study aimed to explore the epidemiology of pathogens in children who were hospitalized with lower respiratory tract infections (LRTIs) at Children's Hospital Fudan University, Shanghai, China. Children aged less than 18 years LRTIs enrolled from January 2013 December 2015. Respiratory specimens collected for detection common viruses, atypical bacteria, and other bacteria using current laboratory diagnostic tests. The epidemiological characteristics analyzed. Of 10,123 obtained patients,...

Abstract Background In addition to rotavirus and norovirus, human adenovirus (HAdV) classic astrovirus (classic HAstV) are important pathogens of acute diarrhea in infants young children. Here, we present the molecular epidemiology HAdV HAstV children with Shanghai. Methods Fecal specimens were collected from 804 outpatient diagnosed Shanghai January 2017 December 2018. All samples screened for presence HAstV. detected using traditional PCR reverse-transcription PCR, respectively. positive...

Pathogens that cause pediatric severe community-acquired pneumonia (SCAP) requiring bronchoscopy intervention are understudied. Through this study, we explore the etiology of SCAP form alveolar lavage fluid (ALF) samples by RespiFinder 2SMART multi-PCR assay.

Human rhinovirus (HRV) is the predominant etiological agent of common cold in children and adults. A recent study showed that inhibitory effect face masks on viral shedding HRV was less prominent than other respiratory viruses. Considering most Chinese people have worn public area since outbreak coronavirus disease 2019, we aimed to find out whether prevailed among 2020 demonstrate details epidemiological features under such a special circumstance.We summarized incidences various virus...

Background During the coronavirus disease 2019 (COVID-19) pandemic, infection of Mycoplasma pneumoniae (MP) decreased significantly. At beginning summer 2023, there was an increasing trend MP in China and pneumonia (MPP) is surging when it comes to school season lasts for several months which has attracted widespread attention. Objective This study aims investigate prevalent characteristics difference between COVID-19 pandemic post Shanghai, China. Methods The demographic information results...

A rapid expansion of hand, foot, and mouth disease (HFMD) outbreaks has occurred caused deaths in China recent years, but little is known about the other etiologic agents except enterovirus 71 (EV71) coxsackievirus 16 (CA16). The objective this study to determine genotype compositions causing HFMD Shanghai identify any associations between types clinical manifestations. Stool specimens were collected from patients hospitalized for treatment HFMD, May 2010 April 2011. Enterovirus was detected...

Sporadic HFMD (hand foot and mouth disease, HFMD) cases outbreaks caused by etiologic agents other than EV71 CA16 have increased globally. We conducted this study to investigate the prevalence genetic characteristics of enteroviruses, especially non-EV71 non-CA16 causing in Shanghai. Clinical specimens were collected from patients with a diagnosis HFMD. A partial length VP1 was amplified RT-PCR subjected direct sequencing. Phylogenetic analyses performed using MEGA 5.0. The ages ranged 3 96...

Abstract Human parechoviruses (HPeVs) are widespread pathogens belonging to the Picornavirus family. The aim of present study was assess prevalence and genetic diversity HPeV in Shanghai, China, during a screening program 2008 2009. Of 300 stool samples from children under age 5 years with acute diarrhea seen at Children's Hospital, Fudan University, 165 (55%) were HPeV‐positive. median infected 3 months. high (57%) infants up 2 old but dropped 30.4% between old. did not differ by sex....

Objectives This study aimed to assess the impact of COVID-19 on prevalence respiratory pathogens among hospitalized children with lower tract infections (LRTIs) in Shanghai. Methods Respiratory specimens were collected from LRTIs Children’s Hospital Fudan University February 2019 January 2021 and common detected using multiplex PCR. The data 13 analyzed compared between year 2020 (from 2021) 2020). Results A total 1,049 patients enrolled, including 417 632 2020. In 2020, 27.53% tested...

To understand virus and host interactions responses to rotavirus infection in children, we analysed by real-time polymerase chain reaction (PCR) the expression of mRNA for five Toll-like receptors (TLRs) (TLR2, TLR3, TLR4, TLR7 TLR8) four T helper (Th)1 Th2 cytokines [interleukin (IL)-2, IL-12, interferon (IFN)-gamma IL-4) peripheral blood mononuclear cells (PBMC) children with acute diarrhoea. We observed significantly higher genes encoding TLR2, TLR8 PBMC 41% (31/75) patients within 3 days...

Noroviruses (NoVs) are considered an important cause of acute gastroenteritis (AGE) across all age groups, especially in children under 5 years age. We investigated the epidemiology noroviruses outpatient from Children's Hospital Fudan University Shanghai, China.Stool specimens were collected between January 2012 and December 2017 1433 with gastroenteritis. All samples analysed by conventional reverse transcription-polymerase chain reaction (RT-PCR) for genogroup II NoVs amplifying both...

Cytomegalovirus (CMV) is the leading cause of congenital infections worldwide and contributes to long-term sequelae in neonates children. CMV envelope glycoproteins play a vital role virus entry cell fusion. The association between polymorphisms clinical outcomes remains controversial. present study aims demonstrate distribution glycoprotein B (gB), H (gH) N (gN) genotypes congenitally (cCMV) infected symptomatic infants attempts figure out viral outcomes.Genotyping gB, gH gN was performed...

Abstract Background Human adenovirus (HAdV) has been recognized as one of the common enteric viruses associated with acute gastroenteritis (AGE) in children. The aim this study was carried out to illustrate epidemiological characterization HAdV Infections among children younger than 15 years Shanghai during COVID-19. Methods During May 2020 and April 2022, 1048 fecal samples were collected from ≤ diagnosed AGE Children’s Hospital Fudan University. identified by PCR sequenced specific...

Abstract Group A rotavirus (RVA) is considered an important cause of acute gastroenteritis (AGE) in all age groups, especially children. We investigated the epidemiology RVA outpatients aged ≤ 16 years at Children's Hospital Fudan University, Shanghai, China. In this study, 16.6% (246/1482) were infected with RVA. The detection rate was significantly higher year 2021 (20.3%, 147/725) compared to 2020 (14.5%, 77/531) and 2022 (9.7%, 22/226) ( p = 0.000). infection prevalent seasons from 2022,...

Background: Human adenovirus (HAdV) is considered a significant enteropathogen associated with sporadic diarrhea in children. However, limited data are available regarding the epidemiology of HAdV hospitalized children viral Shanghai. The aim this study was to characterize HAdVs and describe their association acute Methods: A total 674 fecal samples were subjected PCR or RT-PCR detect RVA, HuCV, HAstV, HAdV. Results: infections detected 4.7% (32/674) specimens, detection rates 13.4% (11/82),...

Since the global outbreak of COVID-19, there has been a significant reduction in pediatric outpatient and emergency visits for infectious diseases. The purpose this study was to analyze changes respiratory viruses children with community-acquired pneumonia (CAP) Shanghai past 10 years, especially first year after COVID-19. We conducted retrospective, observational study; results eight common (respiratory syncytial virus (RSV), influenza A B, parainfluenza 1–3 (PIV), adenovirus (ADV) human...

Our previous studies have shown that vitamin A (VA) status is associated with antiviral immunity and pathogenic conditions in enterovirus 71 (EV71)-infected children. In the present study, we established an vitro model to investigate effects potential mechanism of activity VA. Human monocytic U937 cells were cultured infected EV71. All-trans-retinoic acid (ATRA), active metabolite VA, Ro 41-5253, a retinoic receptor-α (RAR-α) antagonist, used as experimental treatment agents. The percentage...

Objective: To evaluate the diagnostic value of a high-throughput gene targeted amplicon sequencing (TAS) assay for detecting pathogenic microorganisms in alveolar lavage fluid (ALF) from children with severe community-acquired pneumonia (SCAP). Methods: A retrospective study was performed on 48 frozen ALF samples 47 cases admitted to Children's Hospital Fudan University January 1, 2019, March 31, 2019. All were tested by multiplex PCR (Multi-PCR) and TAS assay. The results panels parallel...