A5080741879- Neurogenesis and neuroplasticity mechanisms
- Glycosylation and Glycoproteins Research
- Epigenetics and DNA Methylation
- Proteoglycans and glycosaminoglycans research
- RNA Research and Splicing
- Sphingolipid Metabolism and Signaling
- Genomics and Chromatin Dynamics
- MicroRNA in disease regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- Estrogen and related hormone effects
- Nuclear Structure and Function
- Nerve injury and regeneration
- RNA modifications and cancer
- Signaling Pathways in Disease
- Lipid metabolism and biosynthesis
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sleep and Wakefulness Research
- Alzheimer's disease research and treatments
- Cancer-related molecular mechanisms research
- Neuroscience and Neuropharmacology Research
- Genetics and Neurodevelopmental Disorders
- Pancreatic function and diabetes
- Carbohydrate Chemistry and Synthesis
- Stress Responses and Cortisol
- Tryptophan and brain disorders
Kansai Medical University
2016-2025
Kyoto Prefectural University of Medicine
2012-2013
Hospital for Sick Children
2007-2013
Kansai University
2010
University of Toronto
2007-2008
Osaka Medical Center for Cancer and Cardiovascular Diseases
1997-2004
Osaka University
2002
Hokkaido University
2002
Aichi University of Education
1996-1999
Mammalian sulfoglycolipids comprise two major members, sulfatide (HSO3-3-galactosylceramide) and seminolipid (HSO3-3-monogalactosylalkylacylglycerol). Sulfatide is a lipid component of the myelin sheath serves as epitope for well known oligodendrocyte-marker antibody O4. Seminolipid synthesized in spermatocytes maintained subsequent germ cell stages. Both can be vitro by using isolated cerebroside sulfotransferase. To investigate physiological role to determine whether are biosynthesized...
Myelinated axons are divided into four distinct regions: the node of Ranvier, paranode, juxtaparanode, and internode, each which is characterized by a specific set axonal proteins. Voltage-gated Na<sup>+</sup> channels clustered at high densities nodes, whereas shaker-type K<sup>+</sup> concentrated juxtaparanodal regions. These separated paranodal regions, where septate-like junctions formed between axon myelinating glial cells. Although oligodendrocytes myelin sheaths believed to play an...
We have isolated a cDNA clone encoding human 3′-phosphoadenylylsulfate:galactosylceramide 3′-sulfotransferase (EC 2.8.2.11). Degenerate oligonucleotides, based on amino acid sequence data for the purified enzyme, were used as primers to amplify fragments of gene from renal cancer cell by polymerase chain reaction method. The amplified fragment was then probe screen library. contained an open reading frame 423 acids including all peptides that sequenced. deduced predicts type II transmembrane...
Abstract HSO 3‐3‐galactosylceramide (Sulfatide) species comprise the major glycosphingolipid components of oligodendrocytes and myelin play functional roles in regulation oligodendrocyte maturation formation. Although various sulfatide contain different fatty acids, it is unclear how these affect oligodendrogenesis myelination. The O4 monoclonal antibody reaction with has been widely used as a useful marker for myelin. However, synthesis during pro‐oligodendroblast stage, where...
Abstract In the M‐phase, nuclear membrane is broken down, nucleosomes are condensed as mitotic chromosomes, and transcription factors generally known to be dislocated from their recognition sequences dispersed cytoplasm. However, some have recently been reported remain on chromosomes facilitate rapid re‐activation of target genes in early G1‐phase. Paired‐like homeobox 2B (PHOX2B) a factor exhibiting chromosomal localization during M‐phase. PHOX2B mutations associated with congenital central...
The functional alteration of microglia arises in brains exposed to external stress during early development. Pathophysiological findings neurodevelopmental disorders such as schizophrenia and autism spectrum disorder suggest cerebellar deficits. However, the link between stress-induced reactivity dysfunction is missing. Here, we investigate developmental immune environment translational mouse models that combine two risk factors: maternal infection repeated social defeat (2HIT). We find...
Abstract Galactosylceramide (GalC) and its sulfated analogue, sulfatide, are major galactosphingolipid components of myelin oligodendrocyte plasma membranes in the nervous system. We previously hypothesized that these galactolipids play functional roles regulation terminal differentiation by acting as sensors/transmitters environmental information. Evidence strongly supports this idea. First, molecules initially expressed on cell surface at interface which progenitors first enter...
Abstract Estrogen exerts neuroprotective and promyelinating actions. The therapeutic effect has been shown in animal models of multiple sclerosis, which the myelin sheath is specifically destroyed central nervous system. However, it remains unproven whether estrogen directly involved remyelination via producing cells, oligodendrocytes, or receptors are involved. In this study, we found that membrane‐associated receptor, G protein‐coupled receptor 30 (GPR30), also known as GPER, was expressed...
The accumulation of β-amyloid (Aβ) aggregates in the brain occurs early progression Alzheimer's disease (AD), and non-fibrillar soluble Aβ oligomers are particularly neurotoxic. During binding to fibrils, curcumin, which can exist an equilibrium state between its keto enol tautomers, exists predominantly form, activity form fibrils is much weaker. Here we described strong curcumin derivative Shiga-Y51 shows for scant affinity fibrils. Furthermore, with imaging mass spectrometry revealed...
We have isolated a mouse cDNA clone encoding 3′‐phosphoadenylylsulfate–galactosylceramide 3′‐sulfotransferase (cerebroside sulfotransferase; CST; EC 2.8.2.11) from kidney library, using human CST [Honke, K., Tsuda, M., Hirahara, Y., Ishii, A., Makita, A. & Wada, Y. (1997) J. Biol. Chem. 272 , 4864–4868] as probe. A recombinant protein of the cloned showed activity. The deduced is composed same 423 amino acids and its sequence exhibits 84% identity with that counterpart. Northern‐blot...
Abstract There is general acceptance that the estrogen receptor can act as a transcription factor. However, estrogens also bind to receptors are located at plasma membrane and stimulate rapid intracellular signaling processes. We recently showed membrane‐associated (mER) present within myelin oligodendrocyte (OL) membrane. To understand physiological function of mER in OLs, we investigated its cellular localization involvement CG4 cells OL primary cultures. An ERα was expressed along lacy...
Chondroitin 4-sulfotransferase, which transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate to position 4 of N-acetylgalactosamine in chondroitin, was purified 1900-fold apparent homogeneity with 6.1% yield the serum-free culture medium rat chondrosarcoma cells by affinity chromatography on heparin-Sepharose CL-6B, Matrex gel red A-agarose, 3',5'-ADP-agarose, and second CL-6B. SDS-polyacrylamide electrophoresis enzyme showed two protein bands. Molecular masses these were 60 64 kDa...
We have previously found that the purified chondroitin 6-sulfotransferase (C6ST), which transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of N-acetylgalactosamine in chondroitin, catalyzed sulfation keratan sulfate, and both C6ST activity sulfotransferase (KSST) were expressed COS-7 cells when cDNA was transfected. In this report we describe some properties KSST contained C6ST, characterize sulfated products formed partially desulfated sulfate. Optimal pH,...
Abstract We showed previously that the addition to cultured oligodendrocytes (OLs) of multivalent carbohydrate in form liposomes containing two major glycosphingolipids (GSLs) myelin, galactosylceramide (GalC) and cerebroside sulfate (Sulf), or galactose conjugated bovine serum albumin caused clustering GalC on extracellular surface myelin basic protein (MBP) cytosolic surface. Multivalent also depolymerization actin microfilaments microtubules, indicating interaction with OL transmits a...
Lamins are type V intermediate filament proteins that located beneath the inner nuclear membrane. In mammalian somatic cells, LMNB1 and LMNB2 encode lamins B1 B2, respectively, LMNA gene is alternatively spliced to generate A C. Mutations in lamin genes have been linked many human hereditary diseases, including neurodegenerative disorders. Knowledge about nervous system has accumulated recently, but a precise analysis of subtypes glial cells not yet reported. this study we investigated...
Abstract In the dorsal root ganglia (DRG), two types of glial cells (Schwann and satellite cells) have been identified based on cell morphology expression specific markers. present study, we observed unknown that were positive for p75 neurotrophin receptor (p75NTR), therefore immunohistochemically ultrastructurally characterized first time. These exhibited stronger immunoreactivity against an anti‐p75NTR antibody than DRG neurons (hereafter referred to as p75NTR++ cells). Moreover, these...
Diverse molecular species of sulfatide with differences in FA lengths, unsaturation degrees, and hydroxylation statuses are expressed the kidneys. However, physiological functions specific kidneys unclear. Here, we evaluated distribution their functions. Electron microscopic analysis Cst-deficient mice lacking showed vacuolar accumulation cytoplasm intercalated cells collecting duct, whereas proximal distal tubules were unchanged. Immunohistochemical revealed that H+-ATPase-positive vesicles...
Status epilepticus (SE) induces pathological and morphological changes in the brain. Recently, it has become clear that excessive neuronal excitation, stress drug abuse induce chromatin remodeling neurons, thereby altering gene expression. Chromatin is a key mechanism of epigenetic regulation. Histone H3 phosphorylation frequently used as marker closely related to upregulation mRNA transcription. In present study, we analyzed levels vivo using immunohistochemistry brains mice with...