A5051062216- Liver Disease Diagnosis and Treatment
- Immune cells in cancer
- Cancer Mechanisms and Therapy
- Drug Transport and Resistance Mechanisms
- Cancer Immunotherapy and Biomarkers
- Cancer Cells and Metastasis
- Proteoglycans and glycosaminoglycans research
- Hepatocellular Carcinoma Treatment and Prognosis
- Cancer, Stress, Anesthesia, and Immune Response
- Ferroptosis and cancer prognosis
- Cell Adhesion Molecules Research
- Cancer, Hypoxia, and Metabolism
- Chromatin Remodeling and Cancer
- Glioma Diagnosis and Treatment
- Pancreatic and Hepatic Oncology Research
- Angiogenesis and VEGF in Cancer
- Cytokine Signaling Pathways and Interactions
- Endoplasmic Reticulum Stress and Disease
- Cancer Research and Treatments
- Cancer, Lipids, and Metabolism
- Epigenetics and DNA Methylation
- Nuclear Receptors and Signaling
- Apelin-related biomedical research
- Glycosylation and Glycoproteins Research
- Genomics and Chromatin Dynamics
The University of Texas Southwestern Medical Center
2018-2023
Southwestern Medical Center
2020-2021
Southwestern University
2020
University of Tennessee Health Science Center
2015-2019
Center for Cancer Research
2015-2018
West Virginia University
2011
Morgantown High School
2011
Recent studies have identified a unique cancer-associated fibroblast (CAF) population termed antigen-presenting CAFs (apCAFs), characterized by the expression of major histocompatibility complex class II molecules, suggesting function in regulating tumor immunity. Here, integrating multiple single-cell RNA-sequencing and performing robust lineage-tracing assays, we find that apCAFs are derived from mesothelial cells. During pancreatic cancer progression, cells form downregulating features...
A series of 2-amino-2,3-dihydro-1H-indene-5-carboxamides were designed and synthesized as new selective discoidin domain receptor 1 (DDR1) inhibitors. One the representative compounds, 7f, bound with DDR1 a Kd value 5.9 nM suppressed kinase activity an half-maximal (50%) inhibitory concentration 14.9 nM. 7f potently inhibited collagen-induced signaling epithelial–mesenchymal transition, dose-dependently colony formation pancreatic cancer cells, exhibited promising in vivo therapeutic...
Glioma-Initiating Cells (GICs) are thought to be responsible for tumor initiation, progression and recurrence in glioblastoma (GBM). In previous studies, we reported the constitutive phosphorylation of STAT3 transcription factor GICs derived from GBM patient-derived xenografts, that played a critical role tumorigenesis. this study, show CRISPR/Cas9-mediated deletion an established cell line markedly inhibited tumorigenesis by intracranial injection but had little effect on proliferation...
Immune profiling of tissue through multiplex immunohistochemistry is important for the investigation immune cell dynamics, and it can contribute to disease prognosis evaluation treatment response in cancer patients. However, protocols mouse formalin-fixed, paraffin-embedded have been less successful. Given that formalin fixation paraffin embedding remains most common preparation method processing tissue, this has limited options study system impact novel therapeutics preclinical models. In...
ABSTRACT Although primary tumor control rates after surgery and/or radiation therapy (RT) are generally high in patients with Ewing sarcoma (EWS), those unresectable tumors have failure approaching 30% and experience poorer outcomes. Additionally, although metastatic site irradiation is associated improved survival, dose, volume effects influence the long‐term toxicity risk. Consequently, it important to identify novel systemic agents enhance therapeutic ratio of RT. Given reported DNA...
Glioblastoma multiforme (GBM) is a highly aggressive and malignant brain tumor that refractory to existing therapeutic regimens, which reflects the presence of stem-like cells, termed glioma-initiating cells (GICs). The complex interactions between different signaling pathways epigenetic regulation key genes may be critical in maintaining GICs their state. Although several have been identified as being dysregulated GBM, prognosis GBM patients remains miserable despite improvements targeted...
Malignant glioblastoma (GBM) is a highly aggressive brain tumor with dismal prognosis and limited therapeutic options. Genomic profiling of GBM samples has identified four molecular subtypes (Proneural, Neural, Classical Mesenchymal), which may arise from different stem-like cell (GSC) populations. We previously showed that adherent cultures GSCs grown on laminin-coated plates (Ad-GSCs) spheroid (Sp-GSCs) had high expression stem markers (CD133, Sox2 Nestin), but low differentiation...
Glioblastoma (GBM) is the most common and deadliest primary adult brain tumor. Invasion, resistance to therapy, tumor recurrence in GBM can be attributed part tumor-initiating cells (BTICs). BTICs isolated from various patient-derived xenografts showed high expression of poorly characterized Apelin early ligand A (APELA) gene. Although originally considered a non-coding gene, APELA gene encodes protein that binds receptor promotes growth human embryonic stem formation vasculature. We found...
Metastatic cancer cells adapt to thrive in secondary organs. To investigate metastatic adaptation, we performed transcriptomic analysis of and non-metastatic murine breast cells. We found that pleiotrophin (PTN), a neurotrophic cytokine, is metastasis-associated factor expressed highly by aggressive cancers. Moreover, elevated PTN plasma correlated significantly with metastasis reduced survival patients. Mechanistically, find activates NF-κB leading altered cytokine production, subsequent...
The progression of cancer from localized to metastatic disease is the primary cause morbidity and mortality. interplay between tumor its microenvironment key driver in this process progression. In order for tumors progress metastasize they must reprogram cells that make up promote growth suppress endogenous defense systems, such as immune inflammatory response. We have previously demonstrated stimulation Tsp-1 (TME) potently inhibits Here, we identify a novel tumor-mediated mechanism...
Abstract In the earliest stages of tumor development, epithelial tumors (carcinomas) are physically confined to area tissue in which they form. These nascent lesions (carcinomas situ) sequestered from parenchyma by basement membrane. Within lie a myriad cell types comprised fibroblasts, immune and inflammatory cells endothelial cells. Upon invasion across membrane into parenchyma, must manipulate expression pro- anti-tumorigenic proteins such that pro-tumorigenic factors produced excess...
Abstract Metastatic cancer cells adapt to thrive in secondary organs. To investigate metastatic adaptation, we performed transcriptomic analysis of and non-metastatic murine breast cells. We found that pleiotrophin (PTN), a neurotrophic cytokine, is metastasis-associated factor expressed highly by aggressive cancers. Moreover, elevated PTN plasma correlated significantly with metastasis reduced survival patients. Mechanistically, find activates NF-kB leading altered cytokine production,...
<p>Two animals randomly selected from each group were sacrificed and showed no difference in tumor burden or T cells.</p>
<p>Image analysis pipeline for quantifying CD3, CD4, and CD8 cells in whole liver sections.</p>
<p>Comparative whole liver section images from untreated and IgG control animals stained for CD3, CD4, CD8.</p>
<div>Abstract<p>Cirrhosis is a high-risk state for hepatocellular carcinoma (HCC) development and represents an opportunity to prevent cancer. In the precancerous of cirrhosis, there accumulation neoantigens that may be specifically targetable through immunotherapy. We asked whether immune checkpoint inhibition could tumorigenesis in mouse model diethylnitrosamine carbon tetrachloride–induced HCC. found initiation anti-PD-1 therapy prior up 46% liver tumors. This significant...
<p>Figure Legends for Supplementary Figures S1-S5.</p>
<p>There were no microscopic tumors at initiation of antibody therapy.</p>
<p>Neoantigens tend to increase with worsening fibrosis stage.</p>