A5100638139- Venomous Animal Envenomation and Studies
- Ion channel regulation and function
- Antimicrobial Peptides and Activities
- Nicotinic Acetylcholine Receptors Study
- Biochemical and Structural Characterization
- Cardiac electrophysiology and arrhythmias
- Analytical chemistry methods development
- Electrochemical Analysis and Applications
- Toxin Mechanisms and Immunotoxins
- RNA and protein synthesis mechanisms
- Insect and Pesticide Research
- Long-Term Effects of COVID-19
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- COVID-19 Clinical Research Studies
- interferon and immune responses
- Radioactive element chemistry and processing
- Neuropeptides and Animal Physiology
- Calcium signaling and nucleotide metabolism
- Erythrocyte Function and Pathophysiology
- Hepatitis B Virus Studies
- Spider Taxonomy and Behavior Studies
- Orthoptera Research and Taxonomy
- Signaling Pathways in Disease
- Anesthesia and Sedative Agents
Wuhan University
2015-2024
State Key Laboratory of Virology
2006-2022
Wuhan Institute of Virology
2006-2022
South Central Minzu University
2022
Shenyang Pharmaceutical University
2006-2022
Institut de Biologie Physico-Chimique
2010
Sorbonne Université
2010
Centre National de la Recherche Scientifique
2010
Chinese Academy of Sciences
2008
Wuhan Institute of Physics and Mathematics
2008
Abstract —Extension of the I-band segment titin gives rise to part diastolic force cardiac muscle. Previous studies human transcripts suggested a series differential splicing events in leading so-called N2A and N2B isoform transcripts. Here we investigated expression at protein level wide range mammalian species. Results indicate that myocardium coexpresses 2 distinct isoforms: smaller containing element only (N2B titin) larger with both elements (N2BA titin). The ratio large N2BA small...
Representing a basal branch of arachnids, scorpions are known as 'living fossils' that maintain an ancient anatomy and adapted to have survived extreme climate changes. Here we report the genome sequence Mesobuthus martensii, containing 32,016 protein-coding genes, most among sequenced arthropods. Although M. martensii appears evolve conservatively, it has greater gene family turnover than insects undergone diverse morphological physiological changes, suggesting decoupling molecular...
Abstract Background Lychas mucronatus is one scorpion species widely distributed in Southeast Asia and southern China. Anything hardly known about its venom components, despite the fact that it can often cause human accidents. In this work, we performed a venomous gland transcriptome analysis by constructing screening cDNA library of from Yunnan province compared with previous results Hainan-sourced . Results A total sixteen types peptides proteins are obtained Yunnan-sourced , which greatly...
The potassium channel Kv1.3 is an attractive pharmacological target for autoimmune diseases. Specific peptide inhibitors are key prospects diagnosing and treating these Here, we identified the first scorpion Kunitz-type toxin family with three groups seven members. In addition to their function as trypsin dissociation constants of 140 nm recombinant LmKTT-1a, 160 LmKTT-1b, 124 LmKTT-1c, 136 BmKTT-1, 420 BmKTT-2, 760 BmKTT-3, 107 Hg1, all toxins could block channel. Electrophysiological...
BmKn2 is an antimicrobial peptide (AMP) characterized from the venom of scorpion Mesobuthus martensii Karsch by our group. In this study, Kn2-7 was derived to improve antibacterial activity and decrease hemolytic activity. showed increased inhibitory against both Gram-positive bacteria Gram-negative bacteria. Moreover, exhibited higher clinical antibiotic-resistant strains such as methicillin-resistant Staphylococcus aureus (MRSA). addition, topical use effectively protected skin mice...
Coronaviruses possess a cap structure at the 5' ends of viral genomic RNA and subgenomic RNAs, which is generated through consecutive methylations by virally encoded guanine-N7-methyltransferase (N7-MTase) 2'-O-methyltransferase (2'-O-MTase). The coronaviral N7-MTase unique for its physical linkage with an exoribonuclease (ExoN) harbored in nonstructural protein 14 (nsp14) coronaviruses. In this study, structure-function relationships were analyzed deletion site-directed mutagenesis severe...
Abstract Immunometabolism contributes to inflammation, but how activated macrophages acquire extracellular nutrients fuel inflammation is largely unknown. Here, we show that the plasma membrane potential (V m ) of mediated by Kir2.1, an inwardly-rectifying K + channel, important determinant nutrient acquisition and subsequent metabolic reprogramming promoting inflammation. In absence Kir2.1 activity, depolarized macrophage V lead a caloric restriction state limiting uptake concomitant...
The misuse of antibiotics has led our age to a dangerous edge, as antibiotic-resistant pathogens appear evolve more quickly than are invented. Thus, new agents treat bacterial infection badly needed. Cationic host defense peptides on the first line system and thought be good candidates for treating infection. Here, novel cationic peptide, mucroporin, was cloned characterized from venom Lychas mucronatus. MIC Staphylococcus aureus 25 microg/ml, including pathogens. Based molecular template...
Abstract Scorpion venoms contain a vast untapped reservoir of natural products, which have the potential for medicinal value in drug discovery. In this study, toxin components from scorpion Heterometrus petersii venom were evaluated by transcriptome and proteome analysis. Ten known families peptides proteins identified, include: two potassium channel toxins, four antimicrobial cytolytic peptides, one family each calcium La1‐like phospholipase A2, serine proteases. addition, we also...
The pace of resistance against antibiotics almost exceeds that the development new drugs. As many bacteria have become resistant to conventional antibiotics, drugs or drug resources are badly needed combat antibiotic-resistant pathogens, like methicillin-resistant Staphylococcus aureus (MRSA). Antimicrobial peptides, rich sources existing in nature, able effectively kill multidrug-resistant pathogens. Here, imcroporin, a antimicrobial peptide, was screened and isolated from cDNA library...
Hepatitis B virus (HBV) is a noncytopathic human hepadnavirus that causes acute, chronic hepatitis and hepatocellular carcinoma (HCC). As the clinical utility of current therapies limited, new anti-HBV agents sources for such are still highly sought after. Here, we report Mucroporin-M1, scorpion venom-derived peptide, reduces amount extracellular HBsAg, HBeAg, HBV DNA productions HepG2.2.15 cells in dose-dependent manner inhibits capsid DNA, intracellular RNA replication intermediates Core...