A5102833903- Parathyroid Disorders and Treatments
- Alkaline Phosphatase Research Studies
- Nitric Oxide and Endothelin Effects
- Cardiovascular Disease and Adiposity
- Glaucoma and retinal disorders
- Thyroid Disorders and Treatments
- Diet, Metabolism, and Disease
- Connective tissue disorders research
- Peripheral Artery Disease Management
- Atherosclerosis and Cardiovascular Diseases
- Lipoproteins and Cardiovascular Health
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Cardiac Imaging and Diagnostics
- Cerebrovascular and Carotid Artery Diseases
- Coronary Interventions and Diagnostics
- Aortic Thrombus and Embolism
- Eicosanoids and Hypertension Pharmacology
- Cardiovascular Function and Risk Factors
- Fatty Acid Research and Health
- Dermatological and Skeletal Disorders
- Cholesterol and Lipid Metabolism
- Retinal Development and Disorders
- Hormonal Regulation and Hypertension
- Aortic aneurysm repair treatments
- Acute Ischemic Stroke Management
New York Institute of Technology
2015-2024
University of South Dakota
2011-2024
Sanford Research
2011-2024
Touro University Nevada
2022
Touro University California
2022
Discovery Institute
2015
Sanford Burnham Prebys Medical Discovery Institute
2015
Sanford Health
2015
Howard Hughes Medical Institute
2003-2007
Jackson Laboratory
1998-2003
Glaucoma is a blinding disease usually associated with high intraocular pressure (IOP). In some families, abnormal anterior segment development contributes to glaucoma. The genes causing dysgenesis and glaucoma in most of these families are not identified the affected developmental processes poorly understood. Bone morphogenetic proteins (BMPs) participate various processes. We tested importance Bmp4 gene dosage for ocular Bmp4+/- mice have abnormalities including malformed, absent or...
Background Ectopic vascular calcification is a common condition associated with aging, atherosclerosis, diabetes, and/or chronic kidney disease. Smooth muscle cells are the best characterized source of osteogenic progenitors in vasculature; however, recent studies suggest that endothelial origin can also promote calcification. To test this, we sought to increase potential by overexpressing tissue‐nonspecific alkaline phosphatase ( TNAP ), key enzyme regulates biomineralization, and determine...
Overexpression of tissue-nonspecific alkaline phosphatase (TNAP) in endothelium leads to arterial calcification mice. The purpose this study was examine the effect elevated endothelial TNAP on coronary atherosclerosis. In addition, we aimed endogenous activity human myocardium.A vascular pattern observed non-failing, ischemic, and idiopathic dilated hearts (5 per group); no differences were noted between groups study. Endothelial overexpression achieved mice harboring a homozygous recessive...
Glaucoma is a common disease but its molecular etiology poorly understood. It involves retinal ganglion cell death and optic nerve damage that often associated with elevated intraocular pressure. Identifying genes modify glaucoma phenotypes likely to provide insights mechanisms of glaucoma. We previously reported in DBA/2J mice caused by recessive alleles at two loci, isa ipd, cause iris stromal atrophy pigment dispersion, respectively. A approach for identifying modifier study the effects...
Low thyroid hormone (TH) function has been linked to impaired coronary blood flow, reduced density of small arterioles, and heart failure. Nonetheless, little is known about the mechanisms by which THs regulate microvascular remodeling. The current study examined initial cellular events associated with remodeling induced triiodothyronine (T3) in hypothyroid rats. Rats established hypothyroidism, eight weeks after surgical thyroidectomy (TX), were treated T3 for 36 or 72 hours. early effects...
Objective The purpose of this study was to compare the apolipoprotein composition three major lipoprotein classes in patients with metabolic syndrome healthy controls. Methods Very low density (VLDL), intermediate/low (IDL/LDL, hereafter LDL), and high lipoproteins (HDL) fractions were isolated from plasma 56 subjects 14 age-sex matched volunteers. content analyzed by one-dimensional (1D) gel electrophoresis confirmation a combination mass spectrometry biochemical assays. Results Metabolic...
Background Homoarginine ( hA rg) has been shown to be cardioprotective in a model of ischemic heart failure; however, the mechanism remains unknown. rg can inhibit tissue-nonspecific alkaline phosphatase TNAP ), an enzyme that promotes vascular calcification. We hypothesized will exert beneficial effects by reducing calcification mouse coronary artery disease associated with overexpression and hypercholesterolemia. Methods Results was overexpressed endothelium mice homozygous for low-density...
Background: We have shown that thyroid hormones (THs) are cardioprotective and can be potentially used as safe therapeutic agents for diabetic cardiomyopathy permanent infarction. However, no reliable, clinically translatable protocol exists TH treatment of myocardial ischemia-reperfusion (IR) injury. hypothesized modified low-dose triiodo-L-thyronine (T3) therapy would confer benefits against IR Methods: Adult female rats underwent left coronary artery ligation 60 minutes or sham surgeries....
Abstract Background Nitric oxide synthase 2 (NOS2) contributes to neural death in some settings, but its role glaucoma remains controversial. NOS2 is implicated retinal ganglion cell degeneration a rat model which intraocular pressure (IOP) experimentally elevated by blood vessel cauterization, not where IOP was injection of hypertonic saline. To test the importance for an inherited glaucoma, this study we both genetically and pharmacologically decreased activity DBA/2J mouse model. Methods...
Abstract Thyroid hormones (THs) play a pivotal role in regulating cardiovascular homeostasis. To provide better understanding of the coordinated processes that govern cardiac TH bioavailability, this study investigated influence serum and status on expression transporters cytosolic binding proteins myocardium. In addition, we sought to determine whether administration T3 (instead T4) improves relationship between THs tissue function over short-term treatment period. Adult female Sprague...
Objective We tested the hypothesis that HDL-apolipoprotein A-I exchange (HAE), a measure of high-density lipoprotein (HDL) function and key step in reverse cholesterol transport (RCT), is impaired metabolic syndrome (MetSyn) patients who are asymptomatic for diabetes cardiovascular disease. also compared HAE with cell-based efflux capacity (CEC) to address previous reports CEC enhanced MetSyn populations. Methods ABCA1-specific were measured as tests HDL 60 14 normolipidemic control...
Recent epidemiological cohort studies have suggested that consumption of artificial sweeteners (AS) is associated with adverse cardiovascular events and mortality. However, these population association cannot establish a causal relationship. In this study we investigated the effect long-term (1-year) AS (Equal Splenda, two commonly used AS) on health survival in rats.Adult Sprague-Dawley rats (both sexes, 4-5 months old) were randomized into following 3 groups: control (n = 21), Equal 21)...
In adipose, insulin functions to suppress intracellular lipolysis and secretion of nonesterified fatty acid (NEFA) into plasma. We applied glucose NEFA minimal models (MM) following a frequently sampled intravenous tolerance test (FSIVGTT) assess glucose-specific NEFA-specific resistance. used total individual acids in the MM, comparing model parameters metabolic syndrome (MetSyn) subjects (n = 52) with optimally healthy controls (OptHC; n 14). Results are reported as mean difference (95%...
HDL-apolipoprotein A-I exchange (HAE) measures a functional property associated with HDL’s ability to mediate reverse cholesterol transport. HAE has been used examine HDL function in case-control studies but not of therapeutics that alter particle composition. This study investigates whether niacin and omega-3 fatty acids induce measurable changes using cohort fifty-six subjects metabolic syndrome (MetS) who were previously recruited double-blind trial where they randomized 16 weeks...
Introduction The expression of hundreds genes is altered in response to left ventricular (LV) remodeling following large transmural myocardial infarction (MI). Thyroid hormone (TH) improves LV and cardiac performance after MI. However, the molecular basis unknown. Methods MI was produced by ligation anterior descending coronary artery female SD rats. Rats were divided into groups: (1) Sham MI, (2) (3) MI+T4 treatment (T4 pellet 3.3 mg, 60 days release, implanted subcutaneously immediately...
Oxylipins are metabolites of polyunsaturated fatty acids that mediate cardiovascular health by attenuation inflammation, vascular tone, hemostasis, and thrombosis. Very low-density lipoproteins (VLDL) contain oxylipins, but it is unknown whether the liver regulates their concentrations. In this study, we used a perfused model to observe effect inflammatory lipopolysaccharide (LPS) challenge soluble epoxide hydrolase inhibition (sEHi) on VLDL oxylipins. A compartmental deuterium-labeled...
Vascular dysfunction associated with hypertension comprises hypercontractility and impaired vasodilation. We have previously demonstrated that triiodothyronine (T3), the active form of thyroid hormone, has vasodilatory effects acting through rapid onset mechanisms. In present study, we examined whether T3 mitigates vascular hypertension. To test direct in hypertensive vessels, aortas from female Dahl salt-sensitive (Dahl SS) rats fed a high-salt diet (8% NaCl, HS group) their age-matched...
Background Atherosclerotic cardiovascular disease is prevalent among patients with chronic kidney (CKD). In this study, we initially aimed to test whether vascular calcification associated CKD can worsen atherosclerosis. However, a paradoxical finding emerged from attempting hypothesis in mouse model of adenine-induced CKD. Methods We combined and diet-induced atherosclerosis mice mutation the low-density lipoprotein receptor gene. first were co-treated 0.2% adenine western diet for 8 weeks...