A5068738677- HIV/AIDS drug development and treatment
- HIV Research and Treatment
- RNA Research and Splicing
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Toxin Mechanisms and Immunotoxins
- Cell death mechanisms and regulation
- interferon and immune responses
- Trypanosoma species research and implications
- Biochemical and Molecular Research
- Cardiomyopathy and Myosin Studies
- Immune Response and Inflammation
Harvard University
2020-2023
Laboratoire de Biologie et Pharmacologie Appliquée
2017-2022
École Normale Supérieure Paris-Saclay
2016-2022
Centre National de la Recherche Scientifique
2016-2022
Université Paris-Saclay
2016-2022
Boston VA Research Institute
2020
The mature HIV-1 nucleocapsid protein NCp7 (NC) plays a key role in reverse transcription facilitating the two obligatory strand transfers. Several properties contribute to its efficient chaperon activity: preferential binding single-stranded regions, nucleic acid aggregation, helix destabilization, and rapid dissociation from acids. However, little is known about relationships between these different properties, which are complicated by ability of recognize particular stem–loops, such as...
During HIV-1 assembly and budding, Gag protein, in particular the C-terminal domain containing nucleocapsid (NCd), p1 p6, is site of numerous interactions with viral cellular factors. Most vitro studies have used constructs lacking p6. Here, using NMR spectroscopy, we show that p1-p6 region (NCp15) largely disordered, but interacts transiently NCd. These modify dynamic properties Indeed, isothermal titration calorimetry (ITC), measured a higher entropic penalty to RNA-binding for NCd...
Abstract Most of the translational control gene expression in higher eukaryotes occurs during initiation step protein synthesis. While this process is well characterized mammalian cells, it less defined parasites, including ones that cause human Leishmaniasis. The Leishmania cap-binding isoform 1 (LeishIF4E-1) only binds specific trypanosomatids-specific hypermethylated 5′ cap, called cap-4, stage parasite life cycle. We report here extensive NMR resonance assignment LeishIF4E-1 bound to a...
The nucleocapsid domain (NCd), located at the C-terminus of HIV-1 Gag protein, is involved in numerous stages replication cycle, such as packaging viral genome and reverse transcription. It exists under different forms through life depending on processing by protease. NCd constituted two adjacent zinc knuckles (ZK1 ZK2), separated a flexible linker flanked disordered regions. Here, conformational equilibria between major minor states were highlighted exclusively ZK2, using CPMG CEST NMR...