A5016378732- HIV Research and Treatment
- Cancer therapeutics and mechanisms
- HIV/AIDS drug development and treatment
- DNA and Nucleic Acid Chemistry
- Bacteriophages and microbial interactions
- Bioactive Compounds and Antitumor Agents
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- RNA Research and Splicing
- Advanced biosensing and bioanalysis techniques
- HIV/AIDS Research and Interventions
- RNA modifications and cancer
- Biochemical and Molecular Research
- Genomics and Chromatin Dynamics
- Lung Cancer Research Studies
- Hepatitis B Virus Studies
- Chemical Reactions and Isotopes
- Estrogen and related hormone effects
- Animal Disease Management and Epidemiology
- Plant tissue culture and regeneration
- Animal Virus Infections Studies
- Polyomavirus and related diseases
- Hypothalamic control of reproductive hormones
- CRISPR and Genetic Engineering
- Drug Transport and Resistance Mechanisms
Centre National de la Recherche Scientifique
1986-2021
Sorbonne Université
2006-2021
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2010-2021
Pierre Fabre (France)
2019-2021
Biologie Intégrative des Organismes Marins
2019-2021
Observatoire Océanologique de Banyuls-sur-Mer
2019
Université Paris Cité
1985-2017
Institut Gustave Roussy
2001-2013
Universitat de Barcelona
2008-2010
AIDS United
2010
Besides the nicking-closing (topoisomerase I) activity, an ATP-dependent DNA topoisomerase is present in rat liver nuclei. The enzyme, partially purified, able to catenate invitro closed circles a magnesium-dependent, ATP-dependent, histone Hl-dependent reaction, and decatenate kinetoplast networks yield free minicircles magnesium-dependent reaction. It largely similar other eukaryotic type II topoisomerases its requirements, presumably belongs this class of enzymes. Type I activities were...
Topoisomerase activities have been measured in nuclear extracts of concanavalin A‐stimulated lymphocytes. In parallel with the wave DNA synthesis, type II topoisomerase activity was considerably increased. After 72 h treatment, this stimulated approx. 20‐fold over untreated cells. contrast, I poorly after 24 and 4‐5‐fold h. These findings, together our previous results on regenerating rat liver, suggest a major role replication.
The <i>Bacillus subtilis</i> LrpC protein belongs to the Lrp/AsnC family of transcriptional regulators. It binds upstream region <i>lrpC</i> gene and autoregulates its expression. In this study, we have dissected mechanisms that govern interaction with DNA by electrophoretic mobility shift assay, electron microscopy, atomic force microscopy. is a structure-specific binding forms stable complexes curved sequences containing phased A tracts wraps form spherical, nucleosome-like structures....
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTReverse gyrase of Sulfolobus: purification to homogeneity and characterizationMarc Nadal, Christine Jaxel, Christiane Portemer, Patrick Forterre, Gilles Mirambeau, Michel DuguetCite this: Biochemistry 1988, 27, 26, 9102–9108Publication Date (Print):December 1, 1988Publication History Published online1 May 2002Published inissue 1 December 1988https://pubs.acs.org/doi/10.1021/bi00426a006https://doi.org/10.1021/bi00426a006research-articleACS...
Etoposide, a nonintercalative antitumor drug, is known to inhibit topoisomerase II. Its effects have been tested in concanavalin A stimulated splenocytes, system of cell proliferation which II induced. The primary effect etoposide was strong inhibition DNA synthesis and the production reversible breaks, presumably associated with However, prolonged (20 h) contact drug resulted secondary fragmentation by irreversible double-strand breaks that yielded unusually small fragments. Surprisingly,...
The HIV-1 nucleocapsid is formed during protease (PR)-directed viral maturation, and transformed into pre-integration complexes following reverse transcription in the cytoplasm of infected cell. Here, we report a detailed transmission electron microscopy analysis impact PR transcriptase (RT) on plasticity, using vitro reconstitutions. After binding to nucleic acids, NCp15, proteolytic intermediate protein (NC), was processed at its C-terminus by PR, yielding premature NC (NCp9) followed...
Stretches of guanines can associate in vitro through Hoogsteen hydrogen bonding to form four-stranded structures. In the HIV-1 central DNA flap, generated by reverse transcriptase at end retrotranscription, both two 99 nt-long overlapping (+) strands contain adjacent tracts guanines. This study demonstrates that oligonucleotides containing these G-clusters highly stable G-quadruplexes various structures vitro, whose formation was controlled an easy and reversible protocol using sodium...
Rad51 protein is a well known protagonist of homologous recombination in eukaryotic cells. polymerization on single-stranded DNA and its role presynaptic filament formation have been extensively documented. polymerizes also double-stranded but the significance this remains unclear. We explored behavior Saccharomyces cerevisiae dsDNA influence nucleosomes mechanism to investigate putative chromatin accessibility machinery. combined biochemical approaches, transmission electron microscopy...
ABSTRACT To terminate the reverse transcription of human immunodeficiency virus type 1 (HIV-1) genome, a final step occurs within center proviral DNA generating 99-nucleotide flap (6). This step, catalyzed by transcriptase (RT), is defined as discrete strand displacement (SD) synthesis between first nucleotide after central priming (cPPT) site and position termination sequence (CTS) site. Using recombinant HIV-1 RT circular single-stranded template harboring cPPT-CTS sequence, we have...
A growing number of studies indicate that mRNAs and long ncRNAs can affect protein populations by assembling dynamic ribonucleoprotein (RNP) granules. These phase-separated molecular ‘sponges’, stabilized quinary (transient weak) interactions, control proteins involved in numerous biological functions. Retroviruses such as HIV-1 form self-assembly when their genomic RNA (gRNA) traps Gag GagPol polyprotein precursors. Infectivity requires extracellular budding the particle followed...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMagnesium(2+), Asp-, and Glu- effects in the processive distributive DNA relaxation catalyzed by a eukaryotic topoisomerase IP. Arsene Der Garabedian, Gilles Mirambeau, Jacqueline J. VermeerschCite this: Biochemistry 1991, 30, 41, 9940–9947Publication Date (Print):October 1, 1991Publication History Published online1 May 2002Published inissue 1 October 1991https://doi.org/10.1021/bi00105a018RIGHTS & PERMISSIONSArticle...
ABSTRACT A hepatitis C virus (HCV) epidemic affecting HIV-infected men who have sex with (MSM) is expanding worldwide. In spite of the improved cure rates obtained new direct-acting antiviral drug (DAA) combinations, high rate reinfection within this population calls urgently for novel preventive interventions. study, we determined in cell culture and ex vivo experiments human colorectal tissue that lipoquads, G-quadruplex DNA structures fused to cholesterol, are efficient HCV pangenotypic...
Soraphen A is a myxobacterial metabolite that blocks the acetyl-coenzyme carboxylase of host and was previously identified as novel HIV inhibitor. Here, we report soraphen acts by reducing virus production altering gp120 virion content, impacting entry capacity infectivity. These effects are partially reversed addition palmitic acid, suggesting inhibition envelope palmitoylation one mechanisms antiviral action.
During HIV-1 assembly and budding, Gag protein, in particular the C-terminal domain containing nucleocapsid (NCd), p1 p6, is site of numerous interactions with viral cellular factors. Most vitro studies have used constructs lacking p6. Here, using NMR spectroscopy, we show that p1-p6 region (NCp15) largely disordered, but interacts transiently NCd. These modify dynamic properties Indeed, isothermal titration calorimetry (ITC), measured a higher entropic penalty to RNA-binding for NCd...
New radioimagers, the HRRI (high resolution radioimager) and Phosphorimager (phosphor screen: PS), apt to display more ample linear dose-response scale than radio-sensitive films, were tested in comparaison with quantitative autoradiography (QA). GnRH receptor saturation experiments achieved on tissue sections (rat pituitary, rat brain, human ovary) a iodinate agonist (125l-[D-Ala6, Des-Gly10]-LH-RH Ethylamide) for determination of affinity constant (Kd). In comparable results obtained 3...