A5018508732- Nuclear Receptors and Signaling
- Immunotherapy and Immune Responses
- Macrophage Migration Inhibitory Factor
- RNA Interference and Gene Delivery
- Monoclonal and Polyclonal Antibodies Research
- Cell death mechanisms and regulation
- Phenothiazines and Benzothiazines Synthesis and Activities
- Nanoplatforms for cancer theranostics
- Radiopharmaceutical Chemistry and Applications
- Glycosylation and Glycoproteins Research
- Estrogen and related hormone effects
- Immune Cell Function and Interaction
- Cancer Research and Treatments
- Signaling Pathways in Disease
- Virus-based gene therapy research
- Cytokine Signaling Pathways and Interactions
- Cancer Cells and Metastasis
- Inflammatory mediators and NSAID effects
- Nerve injury and regeneration
- Cancer, Hypoxia, and Metabolism
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Photodynamic Therapy Research Studies
- NF-κB Signaling Pathways
- Cancer Mechanisms and Therapy
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy
2013-2024
Polish Academy of Sciences
2014-2024
Institute of Immunology
2019
New York State Department of Health
1987
Percutaneous coronary intervention has become the most common and widely implemented method of heart revascularization. However, development restenosis remains major limitation this method. Photodynamic therapy (PDT) recently emerged as a new promising for prevention arterial restenosis. Here efficacy chlorin e6 in PDT was investigated vitro using human vascular smooth muscle cells (TG/HA-VSMCs) one cell types crucial PDT-induced death studied on many levels, including annexin V staining,...
Psoriasis is an inflammatory immunogenetic skin disease, often accompanied by itch. Opioid receptors are known regulators of itch sensation in the central nervous system. In brain, μ-opioid may potentiate itch, while activation κ-opioid reduce or even alleviate itch; however, role opioid perception poorly understood. To further elucidate neurobiology psoriatic punch biopsies non-lesional and lesional patients with psoriasis healthy controls were studied. Real-time polymerase chain reaction...
Gadolinium-doped nanoparticles (NPs) are regarded as promising luminescent probes. In this report, we studied details of toxicity mechanism low doses NaGdF₄-based fluorescent in activated RAW264.7, J774A.1 macrophages. These cell lines were specifically sensitive to the treatment with nanoparticles. Using three different sizes, but a uniform zeta potential (about -11 mV), observed rapid uptake NPs by cells, resulting increased lysosomal compartment and subsequent superoxide induction along...
The highly selective multi‐targeted agent sorafenib is an inhibitor of a number intracellular signaling kinases with anti‐proliferative, anti‐angiogenic and pro‐apoptotic effects in various types tumors, including human non‐small cell lung cancer ( NSCLC ). Betulin displays broad spectrum biological pharmacological properties, anticancer chemopreventive activity. Combination drugs different targets logical approach to overcome multilevel cross‐stimulation among key pathways progression....
We established a new B-cell leukaemia cell line CLB70 from dog with chronic lymphocytic leukaemia. This is positive for CD20, CD45, CD79a, MHC class II, IgG, IgM; weakly CD21; and negative CD3, CD4, CD5, CD8, CD14, CD34, CD117. PCR antigen receptor gene rearrangement (PARR) analysis revealed biclonal immunoglobulin heavy chain (IgH) result TCRγ. Western blot of anti- pro-apoptotic proteins showed increased expression Bcl-2, Mcl-1, NF-kB, Ras, decreased p53. cells grow rapidly in vitro are...
The currently approved therapies fail in a substantial number of colorectal cancer (CRC) patients due to the molecular heterogeneity CRC, hence new efficient drug combinations are urgently needed. Emerging data indicate that 5-azanucleosides able sensitize cells standard chemotherapeutic agents and contribute overcoming intrinsic or acquired chemoresistance. CRC with different genetic backgrounds (HCT116, DLD-1, HT-29) were sequentially treated topoisomerase inhibitors. combined effects...
Pancreatic ductal adenocarcinoma (PDAC) is one of the most deadly cancers in world due to late diagnosis and poor response available treatments. It important identify treatment strategies that will increase efficacy reduce toxicity currently used therapeutics. In this study, PDAC cell lines AsPC-1, BxPC-3, Capan-1 were treated with sorafenib betulinic acid alone combination. We examined effect combined treatments on viability (MTS test), proliferation apoptosis (annexin V staining), cycle...
We established a canine natural killer (NK)-type cell line called CNK-89 derived from dog with NK neoplasia. Immunophenotyping analysis showed positive staining for CD5, CD8, CD45, CD56, CD79a and NKp46, while negative CD3, CD4, CD14, CD20, CD21, CD34, Thy1, IgG, IgM MHCII. Polymerase chain reaction revealed the presence of NKG2D, NKp30, NKp44, NKp46 perforin, but absence CD16, Ly49 granzyme B mRNA. Treating cells IL-2 did not change expression activating receptors, TNFα IFNγ secretion...
(1) Background: Decitabine and azacitidine are cytosine analogues representing the class of drugs interfering with DNA methylation. Due to their molecular homology similar clinical application, both often regarded as interchangeable. Despite unique mechanism action studies designed for observation comparison prolonged activity these rare. (2) Methods: The short-time (20-72 h) long-term (up 20 days) anti-cancer decitabine has been studied in colorectal cancer cells. We observe impact on cell...
New predictors of ischemic incidents are constantly sought since they raise the awareness patients and their doctors stroke occurrence. The goal was to verify whether Advanced Glycation End Products (AGEs), in particular AGE10, could be one them. AGE10 measurement conducted using a non-commercial ELISA assay blood serum neurological without cerebrovascular event (n = 24), those with transient brain attack (TIA) 17), severe 35). Twice as many people TIA or presented high concentrations...
Genistein is a natural compound belonging to isoflavone family of secondary plant metabolites, characterized by pleiotropic biological activity. Here we present the results study on new analogs and polysaccharide complexes genistein as potent antiproliferative cell death-inducing agents. Most were 2 (i.e., IFG-027 IFG-043) SPG-G XG-G), which had higher or similar activity in comparison genistein. However, these decreased number cells G2/M phase contrast complex. analogs, IFG-043, also...