A5046505201- Angiogenesis and VEGF in Cancer
- Nanoplatforms for cancer theranostics
- Phenothiazines and Benzothiazines Synthesis and Activities
- Photodynamic Therapy Research Studies
- Macrophage Migration Inhibitory Factor
- Nuclear Receptors and Signaling
- Cancer, Hypoxia, and Metabolism
- Ubiquitin and proteasome pathways
- Nanocluster Synthesis and Applications
- Sphingolipid Metabolism and Signaling
- Advanced biosensing and bioanalysis techniques
- Autophagy in Disease and Therapy
- Porphyrin and Phthalocyanine Chemistry
- Cell death mechanisms and regulation
- Quantum Dots Synthesis And Properties
- Phytochemistry and Bioactivity Studies
- Synthesis and Characterization of Heterocyclic Compounds
- Nanoparticle-Based Drug Delivery
- Quinazolinone synthesis and applications
- Photoacoustic and Ultrasonic Imaging
- Polymer Surface Interaction Studies
- Hippo pathway signaling and YAP/TAZ
- Pharmacological Effects of Natural Compounds
- PI3K/AKT/mTOR signaling in cancer
- RNA Interference and Gene Delivery
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy
2013-2024
Polish Academy of Sciences
2013-2024
Jan Długosz University
2014-2019
McMaster University
2003-2005
Abstract The onset of angiogenesis in cancer often involves down-regulation endogenous inhibitors, which thrombospondin-1 (TSP-1) is a paradigm. As this effect thought to occur under the influence transforming genetic lesions (e.g., expression mutant ras oncogene), its nature regarded as intrinsic cells themselves. Here, we show that ras-transformed can also induce TSP-1 their adjacent nontransformed stromal fibroblasts, but not endothelial cells, paracrine and distance-dependent manner....
Percutaneous coronary intervention has become the most common and widely implemented method of heart revascularization. However, development restenosis remains major limitation this method. Photodynamic therapy (PDT) recently emerged as a new promising for prevention arterial restenosis. Here efficacy chlorin e6 in PDT was investigated vitro using human vascular smooth muscle cells (TG/HA-VSMCs) one cell types crucial PDT-induced death studied on many levels, including annexin V staining,...
Gadolinium-doped nanoparticles (NPs) are regarded as promising luminescent probes. In this report, we studied details of toxicity mechanism low doses NaGdF₄-based fluorescent in activated RAW264.7, J774A.1 macrophages. These cell lines were specifically sensitive to the treatment with nanoparticles. Using three different sizes, but a uniform zeta potential (about -11 mV), observed rapid uptake NPs by cells, resulting increased lysosomal compartment and subsequent superoxide induction along...
The currently approved therapies fail in a substantial number of colorectal cancer (CRC) patients due to the molecular heterogeneity CRC, hence new efficient drug combinations are urgently needed. Emerging data indicate that 5-azanucleosides able sensitize cells standard chemotherapeutic agents and contribute overcoming intrinsic or acquired chemoresistance. CRC with different genetic backgrounds (HCT116, DLD-1, HT-29) were sequentially treated topoisomerase inhibitors. combined effects...
Development of protocols for transferring hydrophobic quantum dots (QDs) into aqueous solution is special importance their biomedical applications. Particularly, hydrophilization Ag2S without quenching photoluminescence a great challenge. Application standard protocol amphiphilic polymer coating nanocrystals failed, whereas ligand exchange and direct synthesis in leads to poorly emitting materials. In this paper we present the facile method QDs employing phase transfer catalysed hydrolysis...
Liposomes serve as delivery systems for biologically active compounds. Existing technologies inefficiently encapsulate large hydrophilic macromolecules, such PVP-conjugated chlorin e6 (Photolon). This photoactive drug has been widely tested therapeutic applications, including photodynamic reduction of atherosclerotic plaque. A novel formulation Photolon was produced using "gel hydration technology". Its pharmacokinetics in Sus scrofa f. domestica. cellular uptake, cytotoxicity, and ability...
Polyamidoamine PAMAM dendrimer generation 3 (G3) was modified by attachment of biotin via amide bond and glucoheptoamidated addition α-D-glucoheptono-1,4-lacton to obtain a series conjugates with variable number residues. The composition determined detailed 1-D 2-D NMR spectroscopy reveal the residues, which were 1, 2, 4, 6, or 8, while glucoheptoamide residues substituted most remaining primary amine groups G3. then used as host molecules encapsulate 5-aminolevulinic acid. solubility acid...
(1) Background: Decitabine and azacitidine are cytosine analogues representing the class of drugs interfering with DNA methylation. Due to their molecular homology similar clinical application, both often regarded as interchangeable. Despite unique mechanism action studies designed for observation comparison prolonged activity these rare. (2) Methods: The short-time (20-72 h) long-term (up 20 days) anti-cancer decitabine has been studied in colorectal cancer cells. We observe impact on cell...
New predictors of ischemic incidents are constantly sought since they raise the awareness patients and their doctors stroke occurrence. The goal was to verify whether Advanced Glycation End Products (AGEs), in particular AGE10, could be one them. AGE10 measurement conducted using a non-commercial ELISA assay blood serum neurological without cerebrovascular event (n = 24), those with transient brain attack (TIA) 17), severe 35). Twice as many people TIA or presented high concentrations...
In our study, we describe the outcomes of intercalation different anthracycline antibiotics in double-stranded DNA at nanoscale and single molecule level. Atomic force microscopy analysis revealed that results significant elongation thinning dsDNA molecules. Additionally, using optical tweezers, have shown decreases stiffness molecules, greater susceptibility to break. Using molecules with GC/AT ratios, checked whether show preference for GC-rich or AT-rich fragments. We found elongation,...
A cyclic tetrapeptide Pro-Pro-Pheβ3ho-Phe (4B8M) was tested for immunosuppressive activity and potential therapeutic utility in several vitro vivo mouse human models. The less toxic splenocytes comparison to cyclosporine (CsA) a parent cyclolinopeptide (CLA). demonstrated potent anti-inflammatory properties antigen-specific skin inflammatory reactions oxazolone toluene diisocyanate as well nonspecific irritants such salicylic acid. It also inhibited processes an air pouch induced by...
Current vascular stents, such as drug eluting stents (DES), have some serious drawbacks, like in stent restenosis and thrombosis. Therefore, other solutions are sought to overcome these post-implantations complications. These include the strategy of biofunctionalization surface with antibodies that facilitate adhesion endothelial cells (ECs) or progenitor (EPCs). Rapid re-endothelialization minimizes risk possible In this study, we proposed ammonium acryloyldimethyltaurate/vinylpyrrolidone...