A5023909101- Protease and Inhibitor Mechanisms
- CAR-T cell therapy research
- Ubiquitin and proteasome pathways
- Viral Infectious Diseases and Gene Expression in Insects
- S100 Proteins and Annexins
- Peptidase Inhibition and Analysis
- Cutaneous lymphoproliferative disorders research
- Cell Adhesion Molecules Research
- Nanowire Synthesis and Applications
- Lymphoma Diagnosis and Treatment
- Protein Degradation and Inhibitors
- Cellular Mechanics and Interactions
- Chronic Lymphocytic Leukemia Research
- Mitochondrial Function and Pathology
- Metabolism and Genetic Disorders
- T-cell and Retrovirus Studies
- ATP Synthase and ATPases Research
- Hedgehog Signaling Pathway Studies
- Nonmelanoma Skin Cancer Studies
- Biosimilars and Bioanalytical Methods
- Autoimmune Bullous Skin Diseases
- Chronic Myeloid Leukemia Treatments
- Neuroblastoma Research and Treatments
- Melanoma and MAPK Pathways
- Cutaneous Melanoma Detection and Management
Helsinki University Hospital
2011-2023
University of Helsinki
2011-2023
Lassila & Tikanoja (Finland)
2023
Finnish Red Cross
2017-2022
University of Oulu
2009-2011
Molina Center for Energy and the Environment
2011
BackgroundAdoptive T-cell therapy offers new options for cancer treatment. Clinical results suggest that persistence, depending on memory, improves efficacy. The use of interleukin (IL)-2 in vitro expansion is not straightforward because it drives effector differentiation but does promote the formation memory. We have developed a cost-effective protocol chimeric antigen receptor (CAR) T cells with an early memory phenotype.MethodsLymphocytes were transduced third-generation lentiviral...
Abstract Lymphatic invasion and accumulation of continuous collagen bundles around tumor cells are associated with poor melanoma prognosis, but the underlying mechanisms molecular determinants have remained unclear. We show here that a copy-number gain or overexpression membrane-type matrix metalloproteinase MMP16 (MT3-MMP) is clinical outcome, bundle assembly cell nests, lymphatic invasion. In cultured WM852 derived from human metastasis, silencing resulted in cell-surface substrate MMP14...
The pathomechanism of mycosis fungoides (MF), the most common type primary cutaneous T-cell lymphomas (CTCLs) and a malignancy non-recirculating, skin-resident T-cells, is unknown albeit underlying viral infections have been sought for. Human endogenous retroviruses (HERVs) are ancient retroviral sequences in human genome their transcription often deregulated cancers. We explored transcriptional activity HERV total 34 samples comprising MF psoriasis skin lesions, as well corresponding...
Abstract Blast-phase chronic myeloid leukemia (BP-CML) is associated with additional chromosomal aberrations, RUNX1 mutations being one of the most common. Tyrosine kinase inhibitor therapy has only limited efficacy in BP-CML, and characterization more defined molecular subtypes warranted order to design better treatment modalities for this poor prognosis patient group. Using whole-exome RNA sequencing we demonstrate that PHF6 BCORL1 mutations, IKZF1 deletions, AID/RAG-mediated...
Indoleamine 2,3-deoxygenase 1 (IDO1) induces immune tolerance in the tumor microenvironment (TME) and is recognized as a potential therapeutic target. We studied expression of both IDO1 related tryptophan 2,3-dioxygenase (TDO) several different subtypes cutaneous T-cell lymphoma (CTCL), evaluated kynurenine (KYN) pathway local TME patient sera. Specimens from total 90 CTCL patients, including mycosis fungoides (MF, n = 37), lymphomatoid papulosis (LyP, 36), primary anaplastic large cell...
Abstract Copy number changes or reduced expression of the Neuron navigator 3 (NAV3) gene occurs in neuroblastomas and malignancies epithelial lymphoid origin. To elucidate whether NAV3 has a role tumorigenesis nervous system tumors general, we studied central peripheral for copy changes. In search common tumorigenic denominators, analyzed 113 tumors, including glial (grades I–IV gliomas), medulloblastomas, neuroblastomas. were by fluorescence situ hybridization correlated to survival...
Subcutaneous panniculitis-like T cell lymphomas represent a rare and difficult to diagnose entity of cutaneous lymphomas. SPTL affects predominantly young adults presents with multifocal subcutaneous nodules frequently associated autoimmune features. The pathogenesis is not completely understood.The aim this study was unravel molecular pathways critical the pathogenesis. Therefore, we analyzed 23 skin samples from 20 newly diagnosed patients relevant control adipose non-malignant...
Abstract: The neuron navigator 3 (NAV3) gene on chromosome 12q21 encodes a microtubule plus end tracking protein and belongs to the family of cytoskeletal regulators. Loss heterozygosity 12q has previously been suggested be associated with poor prognosis in cancers epithelial origin. In this study, we characterized copy number changes NAV3 24 basal cell (BCCs), eight squamous (SCCs) non-malignant inflammatory skin lesions by fluorescent situ hybridization. To identify genes affected NAV3,...
Background: T cells that are genetically modified with chimeric antigen receptor (CAR) hold promise for immunotherapy of cancer. Currently, there intense efforts to improve the safety and efficacy CAR cell therapies against liquid solid tumors. Earlier we designed a novel backbone (FiCAR) where spacer is derived from immunoglobulin (Ig) -like domains signal-regulatory protein alpha (SIRPα). However, analysis using primary slow laborious. Methods: To explore versatility backbone, set variant...
Background: T cells equipped with chimeric antigen receptors (CAR) have shown remarkable efficacy in targeting B lineage malignancies. Improvement of the CAR structure is needed, however, a view to developing flexibly modifiable spacers that are inert interactions unwanted cells. Specifically, binding carrying for IgG’s crystallizable fragment (FcR), recognize IgG-derived domains CARs be avoided. Methods: Two novel CD19 where IgG1-CH2 and -CH3 were replaced Ig-like from signal-regulatory...
Melanoma is a highly metastatic tumour originating from neural crest-derived melanocytes. The aim of this study was to analyse the expression neuron navigator 3 (NAV3) in relation membrane type-1 matrix metalloproteinase MMP14, major regulator invasion, 40 primary melanomas, 15 benign naevi and 2 melanoma cell lines. NAV3 copy number changes were found 18/27 (67%) so that deletions dominated (16/27 samples, 59%). protein be localized at leading edge migrating cells vitro. Silencing reduced...
<p>Supplementary Data. Supplementary Materials and Methods Figure Legends</p>
<p>Supplementary Figures S1-S9. MMP14 which is over-expressed in melanoma cell lines was not associated with patient survival (S1); Vasculature and morphology of human tumors (S2); MMP16 expression WM852 cells its correlation tumor weight xenografts (S3); No significant difference the blood lymphatic vessel densities observed between shScr shMMP16 (S4); MMP16-silencing does affect mouse collagen type I (S5); Endogenous or exogenous regulates invasion cell-cell contacts Bowes (S6);...
<p>Supplementary Data. Supplementary Materials and Methods Figure Legends</p>