A5015662562- Chronic Myeloid Leukemia Treatments
- Glaucoma and retinal disorders
- Retinal Diseases and Treatments
- MicroRNA in disease regulation
- Chronic Lymphocytic Leukemia Research
- Acute Lymphoblastic Leukemia research
- Retinal Imaging and Analysis
- RNA Research and Splicing
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- RNA Interference and Gene Delivery
- Cancer-related molecular mechanisms research
- CAR-T cell therapy research
- Circular RNAs in diseases
- Cytomegalovirus and herpesvirus research
- RNA regulation and disease
- Acute Myeloid Leukemia Research
- Thermodynamic properties of mixtures
- Chemical and Physical Properties in Aqueous Solutions
- Brain Metastases and Treatment
- Connexins and lens biology
- Genetic Associations and Epidemiology
- Ionic liquids properties and applications
- CRISPR and Genetic Engineering
- Renewable energy and sustainable power systems
Flinders University
2022-2024
Centre for Cancer Biology
2011-2023
South Australia Pathology
2011-2023
University of South Australia
2014-2023
Monash Medical Centre
2020
Garvan Institute of Medical Research
2014-2016
The University of Adelaide
2011-2014
The University of Queensland
2000-2011
Keogh Institute for Medical Research
2010
Despite the prevalence of long noncoding RNA (lncRNA) genes in eukaryotic genomes, only a small proportion have been examined for biological function. lncRNAdb, available at http://lncrnadb.org, provides users with comprehensive, manually curated reference database 287 lncRNAs that described independently scientific literature. In addition to capturing great recent literature describing functions individual lncRNAs, lncRNAdb now offers an improved user interface enabling greater...
The ability to alter microRNA (miRNA) abundance is crucial for studying miRNA function. To achieve this there widespread use of both exogenous double-stranded mimics transient over-expression, and single stranded antisense RNAs (antimiRs) inhibition. success these manipulations often assessed using qPCR, but does not accurately report the level functional miRNA. Here, we draw attention discrepancy, which overlooked in many published reports. We measured functionality by comparing total...
The Ago2 component of the RNA-induced silencing complex (RISC) is an endonuclease that cleaves mRNAs base pair with high complementarity to RISC-bound microRNAs. Many examples such direct cleavage have been identified in plants, but not vertebrates, despite conservation catalytic capacity vertebrate Ago2. We performed parallel analysis RNA ends (PAREs), a deep sequencing approach identifies 5'-phosphorylated, polyadenylated RNAs, detect potential microRNA-directed mRNA cleavages mouse embryo...
Abstract Blast-phase chronic myeloid leukemia (BP-CML) is associated with additional chromosomal aberrations, RUNX1 mutations being one of the most common. Tyrosine kinase inhibitor therapy has only limited efficacy in BP-CML, and characterization more defined molecular subtypes warranted order to design better treatment modalities for this poor prognosis patient group. Using whole-exome RNA sequencing we demonstrate that PHF6 BCORL1 mutations, IKZF1 deletions, AID/RAG-mediated...
High-throughput sequencing reveals an abundance of microRNA-sized fragments derived from larger non-coding RNAs. Roles for these small RNAs in gene silencing are suggested by their co-precipitation with Argonaute, the microRNA effector protein, though extent to which they suppress expression endogenously remains unclear. To address this, we used luciferase reporters determine endogenous functionality a diverse range sources. We demonstrate snoRNAs have capacity act microRNA-like manner, note...
The BCR::ABL1 gene fusion initiates chronic myeloid leukemia (CML); however, evidence has accumulated from studies of highly selected cohorts that variants in other cancer-related genes are associated with treatment failure. Nevertheless, the true incidence and impact additional genetic abnormalities (AGA) at diagnosis phase (CP)-CML is unknown. We sought to determine whether AGA a consecutive imatinib-treated cohort 210 patients enrolled TIDEL-II trial influenced outcome despite proactive...
Targeted gene editing to restore CD40L expression via homology-directed repair (HDR) in CD34+ hematopoietic stem and progenitor cells (HSPCs) represents a potential long-term therapy for X-linked hyper IgM syndrome. However, clinical translation of HSPC is limited by inefficient engraftment HDR-edited HSPCs. Here, we ameliorate this issue employing small-molecule inhibitor DNA-PKcs, AZD7648, bias DNA mechanisms facilitate HDR upon CRISPR SpCas9-based editing. Using AZD7648 treatment...
To elucidate a potential association between the apolipoprotein E (APOE) E4 allele and glaucoma prevalence in large cohorts.A cross-sectional analysis of baseline prospectively collected cohort data.UK Biobank (UKBB) participants genetically determined European ancestry (n = 438 711). Replication analyses were performed using clinical genotyping data from recruited to Canadian Longitudinal Study Aging (CLSA; n 18 199), Australian New Zealand Registry Advanced Glaucoma (ANZRAG; 1970), Blue...
Purpose: Pseudoexfoliation syndrome (PEX) is a common systemic disease that results in severe and often irreversible vision loss. Despite considerable research effort, PEX remains incompletely understood. This study sought to perform the first RNAseq elucidate pathophysiology of PEX, contribute publicly available transcriptomic data resource for future research. Methods: Human ocular lens capsular epithelium samples were collected from 25 patients with 39 non-PEX controls undergoing cataract...
To investigate the association between apolipoprotein E (APOE) E4 dementia-risk allele and prospective longitudinal retinal thinning in a cohort study of suspect early manifest glaucoma.Retrospective analysis data.This included all available eyes from participants recruited to Progression Risk Glaucoma: Relevant SNPs [single nucleotide polymorphisms] with Significant Association (PROGRESSA) genotyping data which APOE genotypes could be determined.Apolipoprotein alleles were determined...
Purpose: To assess the association between physical activity and spectral-domain optical coherence tomography (SD-OCT)–measured rates of macular thinning in an adult population with primary open-angle glaucoma. Methods: The correlation accelerometer-measured ganglion cell–inner plexiform layer (GCIPL) was measured 735 eyes from 388 participants Progression Risk Glaucoma: RElevant SNPs Significant Association (PROGRESSA) study. cross-sectional SD-OCT thickness then assessed 8862 6152...
Glaucoma and age-related macular degeneration (AMD) account for a substantial portion of global blindness. Both conditions are highly heritable, with recognised monogenic polygenic inheritance patterns. Current screening guidelines lack decisive recommendations. Polygenic risk scores (PRS) allow cost-effective broad population stratification these conditions. The predictive potential PRS could facilitate earlier diagnosis treatment, prevent unnecessary vision loss.The Genetic Risk Assessment...
The present paper reviews research in the area of broad-spectrum chemotherapeutic agent cisplatin (cis-diamminedichloro-platinum II) and examines implications for clinical neuropsychology arising from neurological disruption associated with cisplatin-based therapy. begins a brief review treatment terms other than survival alone, side-effects potential central nervous system (CNS) dysfunction symptoms concomitant neuropsychology. Two main therapy are identified. First, impacts upon...
Glaucoma is the leading cause of irreversible blindness with early detection and intervention critical to slowing disease progression. However, half those affected are undiagnosed. This largely due stages being asymptomatic; current population-based screening measures unsupported; a lack efficient prediction models. Research investigating polygenic risk scores (PRS) for glaucoma have shown predictive ability identify individuals at higher risk. Potential clinical applications include...